This antipeptide antibody was generated in chickens against a sequence shared between the mouse (P16330) and human (NP_149124) gene products. The antibody was affinity-purified, and its concentration was adjusted to 100 µg/mL. This antibody has been validated with human, mouse and rat tissues.
Volume: 1000 µL
Concentration: 100 µg/mL
Host Species: Chicken
Species Reactivity: Human, Mouse, Rat
Applications: ICC, IHC, WB
Protein Name / Synonyms: 2',3'-cyclic-nucleotide 3'-phosphodiesterase, CNP, CNPase, EC 18.104.22.168
Target Description: Murine CNPase (EC 22.214.171.124) is a 47,122 dalton protein (420 amino acids) found in myelin. This protein is expressed by oligodendrocytes of the CNS and by Schwann cells of the PNS.
Gene ID: CNP
Antibody Registry ID (RRID): AB_2313538
Physical State: Liquid
Production Notes: Chickens were immunized with a synthetic peptide / keyhole limpet hemocyanin (KLH) conjugate. The peptide corresponded to a region of the CNPase gene product shared between the human (NP_149124, NCBI) and mouse (P16330, NCBI) sequences. After repeated injections, immune eggs were collected, the IgY fractions were purified from the yolks, and then affinity-purified using a peptide column. The concentrations of the eluates were then adjusted to 100 µg/mL, and the preparation was filter-sterilized.
Validation and Application Notes
Molecular Weight: 47 kDa
Western Blot Dilution Range: 1:500-1:1000
IHC Dilution Range: 1:500-1:1000
Quality assurance analysis was performed using immunohistochemistry (at a dilution of 1:2000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.
Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). Do not freeze these antibodies unless you want to store them for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half of its binding activity is lost.
Aves Labs products are intended for use as research laboratory reagents. They are not intended for use as diagnostic or therapeutic reagents in humans.
- Beirowski B, Wong KM, Babetto E, Milbrandt J. (2017), 'mTORC1 promotes proliferation of immature Schwann cells and myelin growth of differentiated Schwann cells .' PNAS. 10.1073/pnas.1620761114.
- Lin JP, Mironova YA, Shrager P, Giger RJ. (2017), 'LRP1 regulates peroxisome biogenesis and cholesterol homeostasis in oligodendrocytes and is required for proper CNS myelin development and repair.' eLife. 10.7554/eLife.30498.001.