Immunohistochemical staining of P-zero on a 4.0% paraformaldehyde-fixed, paraffin-embedded tissue section through the sciatic nerve of an adult mouse. (GREEN) P0 concentration was a 1:1000 dilution, followed by fluorescein-labeled goat anti-chicken IgY (1:500 dilution). RED is actin. BLUE is DAPI nuclear counterstain. Photomicrograph from Dr. Gerry Shaw, EnCor Biotechnology.
Chickens were immunized with two synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. These synthetic peptides corresponded to different regions of the Po gene product, but are shared between the human (NP_000521, NCBI) and mouse (NP_032649, NCBI) sequences. After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 200 µg/mL. Finally, equal volumes of both of these affinitypurified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.
Volume: 1000 µL
Concentration: 200 µg/mL
Host Species: Chicken
Species Reactivity: Human, Mouse, Rat
Applications: ICC, IHC, WB
Protein Name / Synonyms: Myelin protein P0 (Myelin peripheral protein) (MPP) (Myelin protein zero)
Gene ID: MPZ
Antibody Registry ID (RRID): AB_2313561
Physical State: Liquid
Validation and Application Notes
Expected Banding Pattern: 27 kDa
Western Blot Dilution Range: 1:10000-1:20000
IHC Dilution Range: 1:2000-1:5000
Both of these anti-peptide antibodies were analyzed by immunohisto-chemistry (at a dilution of 1:2000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.
Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). Do not freeze these antibodies unless you want to store them for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half of its binding activity is lost.
Aves Labs products are intended for use as research laboratory reagents. They are not intended for use as diagnostic or therapeutic reagents in humans.
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