An antipeptide antibody was generated in chickens against a sequence shared between the mouse (P60202) and human (NP_000524) gene products. Antibodies were affinity-purified and the concentration adjusted to 100 µg/mL. These antibodies have been validated with human, mouse and rat tissues.
Volume: 1000 µL
Concentration: 100 µg/mL
Host Species: Chicken
Species Reactivity: Human, Mouse, Rat
Applications: ICC, IHC
Protein Name / Synonyms: Myelin proteolipid protein (PLP) (Lipophilin)
Target Description: Human Proteolipid Protein (PLP, or lipophilin) is a 29,946 dalton protein (277 amino acids) found in myelin of the CNS and PNS. Expressed by oligodendrocytes and Schwann cells, PLP stabilizes myelin by preventing lipid bilayer fusion, and aids in its compaction. Different mutations of the human PLP gene product result in two neurological disorders: Pelizaeus-Merzbacher Disease and Spastic Paraplegia Type 2.
Gene ID: PLP1 PLP
Antibody Registry ID (RRID): AB_2313560
Physical State: Liquid
Production Notes: Chickens were immunized with a synthetic peptide / keyhole limpet hemocyanin (KLH) conjugate. The sequence of the synthetic peptide was shared between the human and mouse gene products. After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, the concentration of the eluate adjusted to 100 µg/mL, and the preparation was filter sterilized.
Validation and Application Notes
Molecular Weight: 30 kDa
Western Blot Dilution Range: 1:2000-1:5000
IHC Dilution Range: 1:2000-1:5000
Each of the three antibodies were analyzed by immunohistochemistry (at a dilution of 1:2000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.
Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). Do not freeze these antibodies unless you want to store them for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half of its binding activity is lost.
Aves Labs products are intended for use as research laboratory reagents. They are not intended for use as diagnostic or therapeutic reagents in humans.
- Lin JP, Mironova YA, Shrager P, Giger RJ. (2017), 'LRP1 regulates peroxisome biogenesis and cholesterol homeostasis in oligodendrocytes and is required for proper CNS myelin development and repair.' eLife. 10.7554/eLife.30498.001.