Chickens were immunized with a synthetic peptide / keyhole limpet hemocyanin (KLH) conjugate. The sequence of the synthetic peptide was shared between the human and mouse gene products. After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, the concentration of the eluate adjusted to 100 µg/mL, and the preparation was filter sterilized.
Volume: 1000 µL
Concentration: 300 µg/mL
Host Species: Chicken
Species Reactivity: Human, Mouse, Rat
Applications: ICC, IHC
Protein Name / Synonyms: Myelin proteolipid protein (PLP) (Lipophilin)
Gene ID: PLP1 PLP
Antibody Registry ID (RRID): AB_2313560
Physical State: Liquid
Validation and Application Notes
Expected Banding Pattern: 30 kDa
Western Blot Dilution Range: 1:2000-1:5000
IHC Dilution Range: 1:2000-1:5000
Each of the three antibodies were analyzed by immunohistochemistry (at a dilution of 1:2000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.
Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). Do not freeze these antibodies unless you want to store them for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half of its binding activity is lost.
Aves Labs products are intended for use as research laboratory reagents. They are not intended for use as diagnostic or therapeutic reagents in humans.
- Lin JP, Mironova YA, Shrager P, Giger RJ. (2017), 'LRP1 regulates peroxisome biogenesis and cholesterol homeostasis in oligodendrocytes and is required for proper CNS myelin development and repair.' eLife. 10.7554/eLife.30498.001.