Adult rat brain immunohistochemistry (with antigen retrieval via sodium citrate pretreatment)

Adult rat brain immunohistochemistry (with antigen retrieval via sodium citrate pretreatment)

Immunoblot versus crude membranes from adult rat brain (RBM) and wild-type (WT) and LRRK2 KO mouse brains probed with 8G10 (left), N241A/34 (middle) and K89/34 (right) TC supe. Mouse brain samples provided by Xiaojie Li, Ted Dawson and Valina Dawson, Johns Hopkins University

Immunoblot versus crude membranes from adult rat brain (RBM) and wild-type (WT) and LRRK2 KO mouse brains probed with 8G10 (left), N241A/34 (middle) and K89/34 (right) TC supe. Mouse brain samples provided by Xiaojie Li, Ted Dawson and Valina Dawson, Johns Hopkins University

from NeuroMab

LRRK2/Dardarin, N-terminus (7x-308)

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Product Details


Detect LRRK2/Dardarin (N-terminus) with this antibody validated for WB, IHC, ICC.


Volume: Purified: 100 µL; Purified Trial: 20 µL; TC Supernatant: 5 mL

Concentration: Purified: 1 mg/mL; TC Supernatant: Lot Specific

Clonality: Monoclonal

Form: Purified and TC Supernatant Available (select your preferred form, size and quantity before clicking "Add to Cart")

Host Species: Mouse

Immunogen: Fusion protein amino acids 100-500 (N-terminus) of human LRRK2 (also known as Leucine-rich repeat serine/threonine-protein kinase 2, Dardarin and PARK8, accession number Q5S007)
Mouse: 82% identity (332/401 amino acids identical)
Rat: 82% identity (330/401 amino acids identical)
30% identity with LRRK1
This NeuroMab antibody is considered "Restricted" and is therefore not available for commercial re-distribution on a for-profit basis.

Target Description: LRRK2 (also known as PARK8) encodes a protein with 5 putative functional domains: an N-terminal leucine-rich repeat (LRR) domain, a Roc (Ras of complex protein) domain that shares sequence homology to the Ras-related GTPase superfamily, a COR (C-terminal of Roc) domain, a mitogen-activated protein kinase kinase kinase (MAPKKK) domain, and a C-terminal WD40 repeat domain. Mutation in this gene is one of the most common causes of inherited Parkinson disease (Gandhi et al., 2008). LRRK2 was originally identified as a putative disease-causing transcript (DKFZp434H2111) within a 2.6-Mb region encompassing a locus for Parkinson disease-8 (PARK8). Northern blot analysis detected a 9-kb mRNA transcript in all tissues tested, including brain. The authors named the protein product dardarin, derived from the Basque word dardara, meaning tremor. LRRK2/dardarin is also known to positively regulate autophagy through a calcium-dependent activation of the CaMKK/AMPK signaling pathway and together with RAB29, plays a role in the retrograde trafficking pathway for recycling proteins, such as mannose 6 phosphate receptor (M6PR), between lysosomes and the Golgi apparatus in a retromer-dependent manner. LRRK2/PARK8 is also known to regulate neuronal process morphology in the intact central nervous system (CNS) and play a role in synaptic vesicle trafficking.

Gene ID: LRRK2 PARK8

Antibody Registry ID (RRID): AB_2315882

Physical State: Liquid

Validation and Application Notes

Molecular Weight: >200 kDa

NOTE

Aves Labs products are intended for use as research laboratory reagents. They are not intended for use as diagnostic or therapeutic reagents in humans.


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