Western blot of human striatal lysate showing specific immunolabeling of the ~88 kDa DAT protein.
from PhosphoSolutions
We are the original manufacturer of our DAT C-terminus rabbit polyclonal antibody, affinity purified from pooled serum. It is optimized in WB, and is an excellent dopaminergic marker for IHC!
Volume: 100 µL (Standard), 25 µL (Trial Size)
Concentration: Lot Specific
Clonality: Polyclonal
Form: Antigen Affinity Purified from Pooled Serum
Host Species: Rabbit
Species Reactivity: Human, Mouse, non-Human primate
Applications: WB, IHC
Immunogen: Synthetic peptide corresponding to amino acid residues from the intracellular C-terminal region conjugated to KLH
Protein Name / Synonyms: DAT
Target Description: The dopamine transporter (DAT) is responsible for the reaccumulation of dopamine after it has been released. DAT antibodies and antibodies for other markers of catecholamine biosynthesis are widely used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson’s disease and drug abuse (Kish et al., 2001; Zhu et al., 2000; Zhu et al., 1999). Levels of DAT protein expression are altered by chronic drug administration (Wilson et al., 1996).
Gene ID: SLC6A3
Antibody Registry ID (RRID): AB_2492076
Physical State: Liquid
Conjugate: Unconjugated
Buffer: 100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
Validation and Application Notes
Molecular Weight: 80
Storage
-20 C
NOTE
Aves Labs products are intended for use as research laboratory reagents. They are not intended for use as diagnostic or therapeutic reagents in humans.