Sph21 neuroblastoma cells in culture.  Fixed with 4% paraformaldehyde.  GAD.1 (GREEN, 1:1000 dilution) immunoreactivity.  Phalloidin (F-actin) (RED) staining.  DAPI (BLUE) staining.  Photomicrograph from Page Balich (Univ. Arizona).

Sph21 neuroblastoma cells in culture. Fixed with 4% paraformaldehyde. GAD.1 (GREEN, 1:1000 dilution) immunoreactivity. Phalloidin (F-actin) (RED) staining. DAPI (BLUE) staining. Photomicrograph from Page Balich (Univ. Arizona).

Immunohistochemical staining of inhibitory neurons (RED; 1:500) within the hippocampal formation of a transgenic, adult male Black6 mouse brain. The brains were fixed with 4.0% paraformaldehyde.  The green staining is low level GFP fluorescence expressed off the actin promoter in this mouse transgenic strain. Secondary antibody (Texas Red goat anti-chicken IgY) was from Jackson Immunoresearch (1:500).

Immunohistochemical staining of inhibitory neurons (RED; 1:500) within the hippocampal formation of a transgenic, adult male Black6 mouse brain. The brains were fixed with 4.0% paraformaldehyde. The green staining is low level GFP fluorescence expressed off the actin promoter in this mouse transgenic strain. Secondary antibody (Texas Red goat anti-chicken IgY) was from Jackson Immunoresearch (1:500).

Immunocytochemical staining of GAD-67 (RED/YELLOW) in cultured cortical neurons from a neonatal mouse (C57/Black6).  GAD staining was detected using Texas Red goat anti-chicken IgY (Jackson Immunoresearch, 1:500).    MAP2 staining (GREEN) used a rabbit antibody (EnCor Biologics; 1:500).  Hoda Ilias, Aves Labs.

Immunocytochemical staining of GAD-67 (RED/YELLOW) in cultured cortical neurons from a neonatal mouse (C57/Black6). GAD staining was detected using Texas Red goat anti-chicken IgY (Jackson Immunoresearch, 1:500). MAP2 staining (GREEN) used a rabbit antibody (EnCor Biologics; 1:500). Hoda Ilias, Aves Labs.

Immunocytochemical staining of GAD-67 (RED/YELLOW) in cultured cortical neurons from a neonatal mouse (C57/Black6).  GAD staining was detected using Texas Red goat anti-chicken IgY (Jackson Immunoresearch, 1:500).    MAP2 staining (GREEN) used a rabbit antibody (EnCor Biologics; 1:500).  Hoda Ilias, Aves Labs.

Immunocytochemical staining of GAD-67 (RED/YELLOW) in cultured cortical neurons from a neonatal mouse (C57/Black6). GAD staining was detected using Texas Red goat anti-chicken IgY (Jackson Immunoresearch, 1:500). MAP2 staining (GREEN) used a rabbit antibody (EnCor Biologics; 1:500). Hoda Ilias, Aves Labs.

Anti-Glutamic Acid Decarboxylase-67 (GAD)

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Details

Chickens were immunized with a synthetic peptide / keyhole limpet hemocyanin (KLH) conjugate. This synthetic peptide corresponded to a region near the Cterminus of this gene product, and was 100% conserved between the human (Q99259, NCBI), mouse (P48318, NCBI) and rat (NP_058703, NCBI) gene products. After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, the concentrations of the eluate adjusted to 100 µg/mL, and the preparation was filter-sterilized through a 0.45 µm filter.

Volume: 1000 µL

Concentration: 100 µg/mL

Clonality: Polyclonal

Host Species: Chicken

Species Reactivity: Human, Mouse, Rat

Applications: ICC, IHC, WB

Protein Name / Synonyms: Glutamate decarboxylase 1 (EC 4.1.1.15) (67 kDa glutamic acid decarboxylase) (GAD-67) (Glutamate decarboxylase 67 kDa isoform)

Gene ID: GAD1 GAD GAD67

Antibody Registry ID (RRID): AB_2313545

Physical State: Liquid

Validation and Application Notes

Molecular Weight: 67 kDa

Western Blot Dilution Range: 1:1000-1:2000

IHC Dilution Range: 1:500-1:1000

Quality Control

This antibody preparation was analyzed by immunohisto-chemistry (at a concentration of 1 µg/mL) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.

Storage

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). Do not freeze these antibodies unless you want to store them for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half of its binding activity is lost.

NOTE

Aves Labs products are intended for use as research laboratory reagents. They are not intended for use as diagnostic or therapeutic reagents in humans.


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