Metabotropic glutamate receptor type 2 (mGluR2, 1:1000 dilution, Aves Labs Cat.# ER2) immunoreactivity (GREEN) and neurofilament-M (NF-M, Rockland, rabbit antibody, 1:500 dilution) immunoreactivity in a tissue section through an e18 mouse cochlear ganglion.
Metabotropic glutamate receptor type 2 (mGluR2, 1:1000 dilution, Aves Labs Cat.# ER2) immunoreactivity (GREEN) and neurofilament-M (NF-M, Rockland, rabbit antibody, 1:500 dilution) immunoreactivity in a tissue section through the CA3 hippocampal formation of an e18 mouse embryo.
Volume: 1000 µL
Concentration: 200 µg/mL
Host Species: Chicken
Species Reactivity: Human, Mouse, Rat
Applications: ICC, IHC
Protein Name / Synonyms: Metabotropic glutamate receptor 2 (mGluR2), Metabotropic glutamate receptor 3 (mGluR3)
Gene ID: GRM2 GPRC1B MGLUR2 (mGluR2) and GRM3 GPRC1C MGLUR3 (mGluR3)
Antibody Registry ID (RRID): AB_2313542
Physical State: Liquid
Production Notes: Chickens were immunized with two synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. These synthetic peptides corresponded to unique sequences within the metabotropic glutamate receptor type 2 and type 3 gene products, but was shared between the human (Q14416.2, Q14832.2 NCBI) and mouse (Q14B12.2, Q9QYS2.1, NCBI) sequences. After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, the concentrations of the eluates adjusted to 200 µg/mL, and the preparation was filter-sterilized.
Validation and Application Notes
IHC Dilution Range: 1:1000-1:2000
This anti-peptide antibody preparation was analyzed by immunohistochemistry (at a dilution of 1:2000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.
Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). Do not freeze these antibodies unless you want to store them for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half of its binding activity is lost.
Aves Labs products are intended for use as research laboratory reagents. They are not intended for use as diagnostic or therapeutic reagents in humans.