MAP-2 immunoreactivity in neurosphere cultures from an e18 mouse brain. MAP-2 is seen in GREEN (1:1000 dilution); 2˚ antibody was a fluorescein-labeled goat anti-chicken IgY (Aves Labs, 1:500 dilution). BLUE staining is DAPI nuclear stain. (Hoda Ilias, Aves Labs.)
Two antipeptide antibodies were generated in chickens against sequences shared between the mouse (P20357) and human (NP_002365) gene products. Antibodies were affinity-purified and mixed in equal concentrations. These antibodies have been validated with human, mouse and rat tissues.
Volume: 1000 µL
Concentration: 200 µg/mL
Host Species: Chicken
Species Reactivity: Human, Mouse, Rat
Applications: ICC, IHC, WB
Protein Name / Synonyms: Microtubule-associated protein 2 (MAP-2)
Target Description: Human MAP-2 is a 199,296 dalton protein (1827 amino acids) expressed in neurons of the PNS and CNS, where it serves as a major component of the neuronal cytoskeleton. MAP-2 contributes to structural integrity and cell shape.
Gene ID: MAP2
Antibody Registry ID (RRID): AB_2313549
Physical State: Liquid
Production Notes: Chickens were immunized with two synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. These synthetic peptides corresponded to different regions of the MAP-2 gene product, but are shared between the human (NP_002365, NCBI) and mouse (P20357, NCBI) sequences. After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 200 µg/mL. Finally, equal volumes of both of these affinitypurified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.
Validation and Application Notes
Molecular Weight: 280 kDa
Western Blot Dilution Range: 1:5000-1:10000
IHC Dilution Range: 1:1000-1:2000
Both of these anti-peptide antibodies were analyzed by immunohisto-chemistry (at a dilution of 1:2000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.
Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). Do not freeze these antibodies unless you want to store them for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half of its binding activity is lost.
Aves Labs products are intended for use as research laboratory reagents. They are not intended for use as diagnostic or therapeutic reagents in humans.
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