MAP-2 immunoreactivity in neurosphere cultures from an e18 mouse brain. MAP-2 is seen in GREEN (1:1000 dilution); 2˚ antibody was a fluorescein-labeled goat anti-chicken IgY (Aves Labs, 1:500 dilution). BLUE staining is DAPI nuclear stain. (Hoda Ilias, Aves Labs.)

MAP-2 immunoreactivity in neurosphere cultures from an e18 mouse brain. MAP-2 is seen in GREEN (1:1000 dilution); 2˚ antibody was a fluorescein-labeled goat anti-chicken IgY (Aves Labs, 1:500 dilution). BLUE staining is DAPI nuclear stain. (Hoda Ilias, Aves Labs.)

Neonatal mouse brain cortical cultures were stained with MAP-2  (GREEN) and AVES LABS GFAP (RED); BLUE is DAPI nuclear stain. Photo courtesy of Dr. Gerry Shaw, Univ. Florida.

Neonatal mouse brain cortical cultures were stained with MAP-2 (GREEN) and AVES LABS GFAP (RED); BLUE is DAPI nuclear stain. Photo courtesy of Dr. Gerry Shaw, Univ. Florida.

Anti-Microtubule-Associated Protein (MAP2)

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Details

Chickens were immunized with two synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. These synthetic peptides corresponded to different regions of the MAP-2 gene product, but are shared between the human (NP_002365, NCBI) and mouse (P20357, NCBI) sequences. After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 200 µg/mL. Finally, equal volumes of both of these affinitypurified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.

Volume: 1000 µL

Concentration: 200 µg/mL

Clonality: Polyclonal

Host Species: Chicken

Species Reactivity: Human, Mouse, Rat

Applications: ICC, IHC, WB

Protein Name / Synonyms: Microtubule-associated protein 2 (MAP-2)

Gene ID: MAP2

Antibody Registry ID (RRID): AB_2313549

Physical State: Liquid

Validation and Application Notes

Molecular Weight: 280 kDa

Western Blot Dilution Range: 1:5000-1:10000

IHC Dilution Range: 1:1000-1:2000

Quality Control

Both of these anti-peptide antibodies were analyzed by immunohisto-chemistry (at a dilution of 1:2000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.

Storage

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). Do not freeze these antibodies unless you want to store them for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half of its binding activity is lost.

NOTE

Aves Labs products are intended for use as research laboratory reagents. They are not intended for use as diagnostic or therapeutic reagents in humans.


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