Chickens were immunized with a recombinant fragment of the human prion protein emulsified with Freund’s complete and incomplete adjuvants.After multiple injections, eggs were collected from the hens, and IgY fractions were prepared from the yolks.Affinity-purified antibodies were then prepared using an agarose column to which the recombinant protein fragments were covalently attached. Finally, affinity purified antibodies (50 µg/mL final concentration) were mixed with the IgY fraction (10 mg/mL final concentration) in a mixture containing glycerol (50% v/v) (to prevent freezing at -20°C) and 0.02% sodium azide.
Volume: 200 µL
Concentration: 10 mg/mL
Form: Mixture of IgY fraction (10 mg/mL) and affinity-purified antibodies (50 µg/mL)
Host Species: Chicken
Species Reactivity: Human, Mouse, Rat
Applications: ICC, IHC, WB
Protein Name / Synonyms: Major prion protein (PrP) (ASCR) (PrP27-30) (PrP33-35C) (CD antigen CD230)
Gene ID: PRNP ALTPRP PRIP PRP
Antibody Registry ID (RRID): AB_2313559
Physical State: Liquid
Validation and Application Notes
IHC Dilution Range: 1:1000-1:2000
Antibodies were analyzed by immunocytochemistry (1:1000 dilution) using cells transfected with the prion protein cDNA.
Store at -20°C in the dark. Under these conditions, the antibodies should have a shelf life of at least 2 years (provided they remain sterile). For longer storage periods, store at -80°C. Note that storage at this lower temperature will destroy some antibody activity due to water crystallization.
Aves Labs products are intended for use as research laboratory reagents. They are not intended for use as diagnostic or therapeutic reagents in humans.