Immunohistochemical staining of SynTag (GREEN, Aves Labs, 1:1000 dilution) and Vimentin (RED, Rockland, 1:500 dilution) in a cryostat section through the basal plate of an e16 mouse brain.
Immunohistochemical staining of SynTag (GREEN, Aves Labs, 1:1000 dilution) and NFH (RED, Rockland, 1:500 dilution) and Vimentin (BLUE) in a cryostat section through the Cochlear nerve and Organ of Corti in an adult mouse.
Immunohistochemical staining of SynTag (GREEN, Aves Labs, 1:1000 dilution) and NFH (RED, Rockland, 1:500 dilution) in a cryostat section through the Cochlear nerve in an adult mouse.
Four different antipeptide antibodies were generated in chickens against sequences shared between the mouse (NP_033332) and human (NP_005630) gene products. Antibodies were affinity-purified and mixed in equal concentrations. These antibodies have been validated with human, mouse and rat tissues.
Volume: 1000 µL
Concentration: 400 µg/mL
Host Species: Chicken
Species Reactivity: Human, Mouse, Rat
Applications: ICC, IHC, WB
Protein Name / Synonyms: Synaptotagmin-1 (Synaptotagmin I) (SytI) (p65)
Target Description: Human Synaptotagmin-1 is a 47,442 dalton protein (422 amino acids) associated with pre-synaptic terminals of the PNS and CNS. Synaptotagmin-1 binds calcium and initiates the fusion of vesicles with the pre-synaptic membrane. This protein also is believed to be the target of proteolytic activity associated with Botulinum neurotoxin A.
Gene ID: SYT1 SVP65 SYT
Antibody Registry ID (RRID): AB_2313562
Physical State: Liquid
Production Notes: Chickens were immunized with four synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. These synthetic peptides corresponded to different regions of the Synaptotagmin-1 gene product, but are shared between the human (NP_005630, NCBI) and mouse (NP_033332, NCBI) sequences. After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentra-tions of the eluates adjusted to 400 µg/mL. Finally, equal volumes of each of the three affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.
Validation and Application Notes
Molecular Weight: 48 kDa
Western Blot Dilution Range: 1:1000-1:2000
IHC Dilution Range: 1:1000-1:2000
Each of the three antibodies were analyzed by immunohistochemistry (at a dilution of 1:2000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.
Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). Do not freeze these antibodies unless you want to store them for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half of its binding activity is lost.
Aves Labs products are intended for use as research laboratory reagents. They are not intended for use as diagnostic or therapeutic reagents in humans.