Immunohistochemical staining of SynTag (GREEN, Aves Labs, 1:1000 dilution) and Vimentin (RED, Rockland, 1:500 dilution) in a cryostat section through the basal plate of an e16 mouse brain.

Immunohistochemical staining of SynTag (GREEN, Aves Labs, 1:1000 dilution) and Vimentin (RED, Rockland, 1:500 dilution) in a cryostat section through the basal plate of an e16 mouse brain.

Immunohistochemical staining of SynTag (GREEN, Aves Labs, 1:1000 dilution) and NFH (RED, Rockland, 1:500 dilution) and Vimentin (BLUE) in a cryostat section through the Cochlear nerve and Organ of Corti in an adult mouse.

Immunohistochemical staining of SynTag (GREEN, Aves Labs, 1:1000 dilution) and NFH (RED, Rockland, 1:500 dilution) and Vimentin (BLUE) in a cryostat section through the Cochlear nerve and Organ of Corti in an adult mouse.

Immunohistochemical staining of SynTag (GREEN, Aves Labs, 1:1000 dilution) and NFH (RED, Rockland, 1:500 dilution) in a cryostat section through the Cochlear nerve in an adult mouse.

Immunohistochemical staining of SynTag (GREEN, Aves Labs, 1:1000 dilution) and NFH (RED, Rockland, 1:500 dilution) in a cryostat section through the Cochlear nerve in an adult mouse.

Western blot of neonatal mouse brain homogenate.  25 ug of total protein added to each lane.  Note the single band at the correct molecular weight.  Hoda Ilias, Aves Labs.

Western blot of neonatal mouse brain homogenate. 25 ug of total protein added to each lane. Note the single band at the correct molecular weight. Hoda Ilias, Aves Labs.

Anti-Synaptotagmin-1 (STG)

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Details

Chickens were immunized with four synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. These synthetic peptides corresponded to different regions of the Synaptotagmin-1 gene product, but are shared between the human (NP_005630, NCBI) and mouse (NP_033332, NCBI) sequences. After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentra-tions of the eluates adjusted to 400 µg/mL. Finally, equal volumes of each of the three affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.

Volume: 1000 µL

Concentration: 400 µg/mL

Clonality: Polyclonal

Host Species: Chicken

Species Reactivity: Human, Mouse, Rat

Applications: ICC, IHC, WB

Protein Name / Synonyms: Synaptotagmin-1 (Synaptotagmin I) (SytI) (p65)

Gene ID: SYT1 SVP65 SYT

Antibody Registry ID (RRID): AB_2313562

Physical State: Liquid

Validation and Application Notes

Molecular Weight: 48 kDa

Western Blot Dilution Range: 1:1000-1:2000

IHC Dilution Range: 1:1000-1:2000

Quality Control

Each of the three antibodies were analyzed by immunohistochemistry (at a dilution of 1:2000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.

Storage

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). Do not freeze these antibodies unless you want to store them for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half of its binding activity is lost.

NOTE

Aves Labs products are intended for use as research laboratory reagents. They are not intended for use as diagnostic or therapeutic reagents in humans.


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