Immunostaining of paraformaldehyde-fixed (4%) paraffin-embedded sections of the substantia nigra pars compacta of an adult mouse brain.  This mouse was engineered to have GFP expressed under control of the actin promoter, which explains the low-level green autofluor-escence.  Note the high number of dopaminergic neuron cell bodies in this brain region.  Detection of the chicken antibody came from a Texas Red-labeled goat anti-chicken IgY (Jackson ImmunoResearch, 1:500).

Immunostaining of paraformaldehyde-fixed (4%) paraffin-embedded sections of the substantia nigra pars compacta of an adult mouse brain. This mouse was engineered to have GFP expressed under control of the actin promoter, which explains the low-level green autofluor-escence. Note the high number of dopaminergic neuron cell bodies in this brain region. Detection of the chicken antibody came from a Texas Red-labeled goat anti-chicken IgY (Jackson ImmunoResearch, 1:500).

Immunostaining of unfixed, thick vibratome sections of adult rat brain Rostral Ventral Lateral Medulla (RVLM) with anti-TH antibody (Aves Labs, 1:1000 dilution), followed by HRP-labeled goat anti-chicken IgY secondary antibody (Aves Labs, 1:1000 dilution).

Immunostaining of unfixed, thick vibratome sections of adult rat brain Rostral Ventral Lateral Medulla (RVLM) with anti-TH antibody (Aves Labs, 1:1000 dilution), followed by HRP-labeled goat anti-chicken IgY secondary antibody (Aves Labs, 1:1000 dilution).

Immunostaining of unfixed, thick vibratome sections of adult rat brain Rostral Ventral Lateral Medulla (RVLM) with anti-TH antibody (Aves Labs, 1:1000 dilution), followed by HRP-labeled goat anti-chicken IgY secondary antibody (Aves Labs, 1:1000 dilution).

Immunostaining of unfixed, thick vibratome sections of adult rat brain Rostral Ventral Lateral Medulla (RVLM) with anti-TH antibody (Aves Labs, 1:1000 dilution), followed by HRP-labeled goat anti-chicken IgY secondary antibody (Aves Labs, 1:1000 dilution).

Immunostaining of unfixed, thick vibratome sections of adult rat brain Rostral Ventral Lateral Medulla (RVLM) with anti-TH antibody (Aves Labs, 1:1000 dilution), followed by HRP-labeled goat anti-chicken IgY secondary antibody (Aves Labs, 1:1000 dilution).

Immunostaining of unfixed, thick vibratome sections of adult rat brain Rostral Ventral Lateral Medulla (RVLM) with anti-TH antibody (Aves Labs, 1:1000 dilution), followed by HRP-labeled goat anti-chicken IgY secondary antibody (Aves Labs, 1:1000 dilution).

Immunostaining of paraformaldehyde-fixed (4%) paraffin-embedded sections of the caudate nucleus of an adult mouse brain.  This mouse was engineered to have GFP expressed under control of the actin promoter, which explains the low-level green autofluorescence.  Note the high number of dopaminergic fibers in this brain region. Detection of the chicken antibody came from a Texas Red-labeled goat anti-chicken IgY (Jackson ImmunoResearch, 1:500).

Immunostaining of paraformaldehyde-fixed (4%) paraffin-embedded sections of the caudate nucleus of an adult mouse brain. This mouse was engineered to have GFP expressed under control of the actin promoter, which explains the low-level green autofluorescence. Note the high number of dopaminergic fibers in this brain region. Detection of the chicken antibody came from a Texas Red-labeled goat anti-chicken IgY (Jackson ImmunoResearch, 1:500).

Immunostaining of paraformaldehyde-fixed (4%) paraffin-embedded sections of the caudate nucleus of an adult mouse brain.  This mouse was engineered to have GFP expressed under control of the actin promoter, which explains the low-level green autofluorescence.  Note the high number of dopaminergic fibers in this brain region.  Detection of the chicken antibody came from a Texas Red-labeled goat anti-chicken IgY (Jackson ImmunoResearch, 1:500).

Immunostaining of paraformaldehyde-fixed (4%) paraffin-embedded sections of the caudate nucleus of an adult mouse brain. This mouse was engineered to have GFP expressed under control of the actin promoter, which explains the low-level green autofluorescence. Note the high number of dopaminergic fibers in this brain region. Detection of the chicken antibody came from a Texas Red-labeled goat anti-chicken IgY (Jackson ImmunoResearch, 1:500).

Immunostaining of paraformaldehyde-fixed (4%) paraffin-embedded sections of the caudate nucleus of an adult mouse brain.  This mouse was engineered to have GFP expressed under control of the actin promoter, which explains the low-level green autofluorescence.  Note the high number of dopaminergic fibers in this brain region.  Detection of the chicken antibody came from a Texas Red-labeled goat anti-chicken IgY (Jackson ImmunoResearch, 1:500).

Immunostaining of paraformaldehyde-fixed (4%) paraffin-embedded sections of the caudate nucleus of an adult mouse brain. This mouse was engineered to have GFP expressed under control of the actin promoter, which explains the low-level green autofluorescence. Note the high number of dopaminergic fibers in this brain region. Detection of the chicken antibody came from a Texas Red-labeled goat anti-chicken IgY (Jackson ImmunoResearch, 1:500).

Immunostaining of paraformaldehyde-fixed (4%) paraffin-embedded sections of the substantia nigra pars compacta of an adult mouse brain.  This mouse was engineered to have GFP expressed under control of the actin promoter, which explains the low-level green autofluor-escence.  Note the high number of dopaminergic neuron cell bodies in this brain region.  Detection of the chicken antibody came from a Texas Red-labeled goat anti-chicken IgY (Jackson ImmunoResearch, 1:500).

Immunostaining of paraformaldehyde-fixed (4%) paraffin-embedded sections of the substantia nigra pars compacta of an adult mouse brain. This mouse was engineered to have GFP expressed under control of the actin promoter, which explains the low-level green autofluor-escence. Note the high number of dopaminergic neuron cell bodies in this brain region. Detection of the chicken antibody came from a Texas Red-labeled goat anti-chicken IgY (Jackson ImmunoResearch, 1:500).

Immunostaining of paraformaldehyde-fixed (4%) paraffin-embedded sections of the substantia nigra pars compacta of an adult mouse brain.  This mouse was engineered to have GFP expressed under control of the actin promoter, which explains the low-level green autofluor-escence.  Note the high number of dopaminergic neuron cell bodies in this brain region.  Detection of the chicken antibody came from a Texas Red-labeled goat anti-chicken IgY (Jackson ImmunoResearch, 1:500).

Immunostaining of paraformaldehyde-fixed (4%) paraffin-embedded sections of the substantia nigra pars compacta of an adult mouse brain. This mouse was engineered to have GFP expressed under control of the actin promoter, which explains the low-level green autofluor-escence. Note the high number of dopaminergic neuron cell bodies in this brain region. Detection of the chicken antibody came from a Texas Red-labeled goat anti-chicken IgY (Jackson ImmunoResearch, 1:500).

Anti-Tyrosine Hydroxylase (TYH)

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Details

Chickens were immunized with two synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. These synthetic peptides corresponded to different regions of the Tyrosine Hydroxylase gene product, but were shared between the human (P07101, NCBI) and mouse (P24529, NCBI) sequences. After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 200 µg/mL. Finally, equal volumes of both affinity-purified anti-peptide antibody preparations were mixed, and the preparation was filter-sterilized.

Volume: 1000 µL

Concentration: 200 µg/mL

Clonality: Polyclonal

Host Species: Chicken

Species Reactivity: Human, Mouse, Rat

Applications: ICC, IHC, WB

Protein Name / Synonyms: Tyrosine 3-monooxygenase (EC 1.14.16.2) (Tyrosine 3-hydroxylase) (TH)

Gene ID: TH TYH

Antibody Registry ID (RRID): AB_10013440

Physical State: Liquid

Validation and Application Notes

Molecular Weight: 60 kDa

Western Blot Dilution Range: 1:1000-1:2000

IHC Dilution Range: 1:1000-1:2000

Quality Control

Both of the antibodies were analyzed by immunohistochemistry (at a concentration of 3 µg/mL) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.

Storage

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). Do not freeze these antibodies unless you want to store them for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half of its binding activity is lost.

NOTE

Aves Labs products are intended for use as research laboratory reagents. They are not intended for use as diagnostic or therapeutic reagents in humans.


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