We are the original manufacturer of our CREB (Ser133) rabbit polyclonal phosphospecific antibody, affinity purified from pooled serum. Optimized in WB and IHC.
Volume: 100 µL (Standard), 25 µL (Trial Size)
Concentration: Lot Specific
Clonality: Polyclonal
Form: Antigen Affinity Purified from Pooled Serum
Host Species: Rabbit
Species Reactivity: Rat
Applications: WB, IHC
Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser133 conjugated to KLH
Protein Name / Synonyms: p-creb, pcreb
Target Description: It is well known that the control of gene expression involves activation of protein kinase cascades that regulate transcription factors within the nucleus (Karin and Hunter, 1995). The cyclic AMP response element binding protein (CREB) is one of the best characterized stimulus-induced transcription factors (Montminy, 1997). This transcription factor is a component of intracellular signaling events that regulate a wide range of biological functions, from spermatogenesis to circadian rhythms and memory (Shaywitz and Greenberg, 1999; Silva et al., 1998). A variety of protein kinases including protein kinase A (PKA), mitogen-activated protein kinases (MAPKs), and Ca2+/calmodulin-dependent protein kinases (CaMKs) phosphorylate CREB at serine 133 (Ser-133), and phosphorylation of Ser-133 are required for CREB-mediated transcription (Johannessen et al., 2004; Kornhauser et al., 2002).
Gene ID: CREB1
Antibody Registry ID (RRID): AB_2492066
Physical State: Liquid
Conjugate: Unconjugated
Buffer: 100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
Validation and Application Notes
Molecular Weight: 45
Storage
-20 C
NOTE
Aves Labs products are intended for use as research laboratory reagents. They are not intended for use as diagnostic or therapeutic reagents in humans.