Immunostaining of primary rat cortical neuroepithelial cells with anti-Olig2 (cat # 1538-OLIG2, red, 1:1000). The cells were treated with basic FGF for 30 hours prior to staining (this induces Olig2 expression in these primary cells).
Our Olig 2 rabbit polyclonal total protein antibody is Protein A purified. It is excellent for use in WB, IHC, IP, and ICC.
Volume: 100 µL (Standard), 25 µL (Trial Size)
Concentration: Lot Specific
Form: Protein A purified
Host Species: Rabbit
Species Reactivity: Human, Mouse, Rat
Applications: WB, IHC, IP
Immunogen: Recombinant mouse Olig2
Protein Name / Synonyms: Olig
Target Description: Olig2 is a transcription factor which is required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. Expressed in the ventral spinal cord as early as 9.5 dpc and scattered in the mantle zone, likely corresponding to oligodendrocyte progenitors migrating out from their site of origin. From 10.5 through 14.5 dpc, Olig2 is expressed in numerous cells in the ventricular and subventricular zones of the lateral and medial ganglionic eminences, suggesting that expression might not be limited to the oligodendrocytic lineage. Olig2 has further been shown to be critical in the proliferation of malignant glioma in brain tumors (Ligon et al., 2007)
Gene ID: OLIG2
Antibody Registry ID (RRID): AB_2492193
Physical State: Liquid
Buffer: 100 µl in 100 mM Glycine, 200 mM Tris, pH 8.0
Validation and Application Notes
Molecular Weight: 27
Aves Labs products are intended for use as research laboratory reagents. They are not intended for use as diagnostic or therapeutic reagents in humans.