Immunofluorescence of TREM2-FLAG transfected COS-7 cells using chicken α-TREM2 (green) and mouse α-flag tag (red). Yellow/orange staining shows 100% correspondence between the two antibodies for recognition of transfected cells. Blue staining is DAPI and stains nuclei of both transfected and untransfected cells.
Our anti-TREM2 chicken primary antibody from AvesLabs is manufactured in-house. It detects TREM2, and is purified. It is provided in liquid form.
Volume: 100µL (TREM2-0020) or 500µL (TREM2-0100)
Host Species: Chicken
Antibody Isotype: IgY
Species Reactivity: Human, Mouse, Rat
Applications: ICC, WB
Immunogen: Purified recombinant extracellular domain of human TREM2 (Uniprot Q9NZC2) expressed in E.Coli
Protein Name / Synonyms: Triggering receptor expressed on myeloid cells 2, TREM-2, Triggering receptor expressed on monocytes 2
Target Description: Triggering receptor expressed on myeloid cells 2, TREM2, is a type I transmembrane innate immune receptor predominantly expressed by microglia in the central nervous system and is genetically linked to the risk for Alzheimer’s disease (Schmid, C.D., et al., 2002). TREM2 has been shown to be a specific marker for alternatively activated microglia known as M2 cells (Cherry, J.D. et al., 2014). TREM2 binds to its adaptor, DNAX-activating protein (DAP12) on the surface of microglia to enact innate immune responses and downstream cellular responses or signaling pathways (Jay,T.R, et al., 2017). TREM2 has important roles in microglial phagocytosis of apoptotic neurons, damaged myelin, and amyloid plaques (Gratuze, M., et al., 2018). In early brain development, TREM2 is essential for synaptic pruning (Filipello, F. et al., 2018).
Gene ID: TREM2
Physical State: Liquid
Production Notes: Eggs from hens hyperimmunized with target were used to prepare an IgY fraction which was then subjected to antigen-specific affinity purification
Validation and Application Notes
Molecular Weight: 36 kDa
Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.
Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. Do not freeze these antibodies unless you want to store them for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half of its binding activity is lost.
Aves Labs products are intended for use as research laboratory reagents. They are not intended for use as diagnostic or therapeutic reagents in humans.