Volume: Purified: 100 µL; Purified Trial: 20 µL; TC Supernatant: 5 mL

Concentration: Purified: 1 mg/mL; TC Supernatant: Lot Specific

Clonality: Monoclonal

Form: Purified and TC Supernatant Available (select your preferred form, size and quantity before clicking "Add to Cart")

Host Species: Mouse

Immunogen: Lipid raft fractions isolated from adult rat brain. Target identified as Neurofascin by: - binding to NF-186-tranfected COS cells- recognition of NF155 and NF186 but not other highly related L1 CAMs like NrCAM- immunoprecipitation of NF155 and NF186 and confirmation by mass spectrometry - loss of immunostaining against NF186-shRNA knockdown samples Rat: amino acids 25-1110 (extracellular domain common to NF155 and NF186)
Mouse: 98% identity (1074/1086 amino acids identical)
Human: 79% identity (955/1195 amino acids identical)
This NeuroMab antibody is considered "Restricted" and is therefore not available for commercial re-distribution on a for-profit basis.

Target Description: Neurofascin is encoded by the gene NFASC. Two major isoforms of NeuroFascin are expressed as a result of alternative splicing, NF186 and NF155. These proteins are expressed in the central nervous system. In brain, NF186 is expressed at Nodes of Ranvier andNF155 is expressed in unmyelinated axons. Neuofascin plays a role in cell adhesion and is thought to be involved in processes such as neurite extension, synaptogenesis and myelination. Diseases associated with this gene include Demyelinating Polyneuropathy.

Gene ID: Nfasc

Antibody Registry ID (RRID): AB_2282826

Physical State: Liquid

Validation and Application Notes

Molecular Weight: 155, 186 kDa

NOTE

Aves Labs products are intended for use as research laboratory reagents. They are not intended for use as diagnostic or therapeutic reagents in humans.

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Citations

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• Franssen EH, Zhao RR, Koseki H, Kanamarlapudi V, Hoogenraad CC, Eva R, Fawcett JW. (2015), 'Exclusion of integrins from CNS axons is regulated by Arf6 activation and the AIS..' Journal of Neuroscience. 10.1523/JNEUROSCI.2850-14.2015.
• Albrecht D, Winterflood CM, Sadeghi M, Tschager T, Noé F, Ewers H. (2016), 'Nanoscopic compartmentalization of membrane protein motion at the axon initial segment..' The Journal of Biology. 10.1083/jcb.201603108.
• Sohn PD, Tracy TE, Son HI, Zhou Y, Leite RE, Miller BL, Seeley WW, Grinberg LT, Gan L. (2016), 'Acetylated tau destabilizes the cytoskeleton in the axon initial segment and is mislocalized to the somatodendritic compartment..' Molecular Neurodegeneration. 10.1186/s13024-016-0109-0.
• Sidenstein SC, D'Este E, Böhm MJ, Danzl JG, Belov VN, Hell SW. (2016), 'Multicolour Multilevel STED nanoscopy of Actin/Spectrin Organization at Synapses..' Scientific Reports. 10.1038/srep26725.
• Benned-Jensen T, Christensen RK, Denti F, Perrier JF, Rasmussen HB, Olesen SP. (2016), 'Live Imaging of Kv7.2/7.3 Cell Surface Dynamics at the Axon Initial Segment: High Steady-State Stability and Calpain-Dependent Excitotoxic Downregulation Revealed..' Journal of Neuroscience. 10.1523/JNEUROSCI.2631-15.2016.
• Evans MD, Dumitrescu AS, Kruijssen DLH, Taylor SE, Grubb MS. (2015), 'Rapid Modulation of Axon Initial Segment Length Influences Repetitive Spike Firing..' Cell Report. 10.1016/j.celrep.2015.09.066.
• Ganguly A, Tang Y, Wang L, Ladt K, Loi J, Dargent B, Leterrier C, Roy S. (2015), 'A dynamic formin-dependent deep F-actin network in axons..' The Journal of Biology. 10.1083/jcb.201506110.
• Papandréou MJ, Vacher H, Fache MP, Klingler E, Rueda-Boroni F, Ferracci G, Debarnot C, Pipéroglou C, Garcia Del Caño G, Goutebroze L, Dargent B. (2015), 'CK2-regulated schwannomin-interacting protein IQCJ-SCHIP-1 association with AnkG contributes to the maintenance of the axon initial segment..' Journal of Neurochemistry. 10.1111/jnc.13158.
• D'Este E, Kamin D, Göttfert F, El-Hady A, Hell SW. (2015), 'STED nanoscopy reveals the ubiquity of subcortical cytoskeleton periodicity in living neurons..' Cell Report. 10.1016/j.celrep.2015.02.007.
• Freeman SA, Desmazières A, Simonnet J, Gatta M, Pfeiffer F, Aigrot MS, Rappeneau Q, Guerreiro S, Michel PP, Yanagawa Y, Barbin G, Brophy PJ, Fricker D, Lubetzki C, Sol-Foulon N. (2015), 'Acceleration of conduction velocity linked to clustering of nodal components precedes myelination..' Proceedings of the National Academy of Science of the United States of America. 10.1073/pnas.1419099112.
• Jain S, Farías GG, Bonifacino JS. (2015), 'Polarized sorting of the copper transporter ATP7B in neurons mediated by recognition of a dileucine signal by AP-1..' Molecular Biology of the Cell. 10.1091/mbc.E14-07-1177.
• Notturno F, Di Febo T, Yuki N, Fernandez Rodriguez BM, Corti D, Nobile-Orazio E, Carpo M, De Lauretis A, Uncini A. (2014), 'Autoantibodies to neurofascin-186 and gliomedin in multifocal motor neuropathy..' Journal of Neuroimmunology. 10.1016/j.jneuroim.2014.09.001.
• Muir J, Kittler JT. (2014), 'Plasticity of GABAA receptor diffusion dynamics at the axon initial segment..' Frontiers in Neuroscience. 10.3389/fncel.2014.00151.
• Lukinavičius G, Reymond L, D'Este E, Masharina A, Göttfert F, Ta H, Güther A, Fournier M, Rizzo S, Waldmann H, Blaukopf C, Sommer C, Gerlich DW, Arndt HD, Hell SW, Johnsson K. (2014), 'Fluorogenic probes for live-cell imaging of the cytoskeleton..' Nature Methods. 10.1038/nmeth.2972.
• Jones SL, Korobova F, Svitkina T. (2014), 'Axon initial segment cytoskeleton comprises a multiprotein submembranous coat containing sparse actin filaments..' The Journal of Biology. 10.1083/jcb.201401045.
• Yap CC, Vakulenko M, Kruczek K, Motamedi B, Digilio L, Liu JS, Winckler B. (2012), 'Doublecortin (DCX) mediates endocytosis of neurofascin independently of microtubule binding..' Journal of Neuroscience. 10.1523/JNEUROSCI.5318-11.2012.
• Galiano MR, Jha S, Ho TS, Zhang C, Ogawa Y, Chang KJ, Stankewich MC, Mohler PJ, Rasband MN. (2012), 'A distal axonal cytoskeleton forms an intra-axonal boundary that controls axon initial segment assembly..' Cell. 10.1016/j.cell.2012.03.039.
• Dhaunchak AS, Becker C, Schulman H, De Faria O Jr, Rajasekharan S, Banwell B, Colman DR, Bar-Or A; Canadian Pediatric Demyelinating Disease Group. (2012), 'Implication of perturbed axoglial apparatus in early pediatric multiple sclerosis..' Annals of Neurology. 10.1002/ana.22693.
• Dhaunchak AS, Huang JK, De Faria Junior O, Roth AD, Pedraza L, Antel JP, Bar-Or A, Colman DR. (2010), 'A proteome map of axoglial specializations isolated and purified from human central nervous system..' Glia. 10.1002/glia.21064.
• Hedstrom KL, Ogawa Y, Rasband MN. (2008), 'AnkyrinG is required for maintenance of the axon initial segment and neuronal polarity..' The Journal of Biology. 10.1083/jcb.200806112.

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