Immunofluorescence staining of cultured rat hippocampal neurons with K28/43 (green) and K57/1 (red, Kv4.2), right image is higher magnification of left image (dotted lines).

Immunofluorescence staining of cultured rat hippocampal neurons with K28/43 (green) and K57/1 (red, Kv4.2), right image is higher magnification of left image (dotted lines).

Electron micrograph of K28/43 hippocampal labelling using a post-embedding immunogold method. Immunoparticles (arrows) are seen in the postsynaptic densities of dendritic spines (s) forming asymmetrical synapses with axon terminals (b). Scale bar = 200 nm. Image courtesy of Rafael Lujan (Universidad de Castilla-La Mancha).

Electron micrograph of K28/43 hippocampal labelling using a post-embedding immunogold method. Immunoparticles (arrows) are seen in the postsynaptic densities of dendritic spines (s) forming asymmetrical synapses with axon terminals (b). Scale bar = 200 nm. Image courtesy of Rafael Lujan (Universidad de Castilla-La Mancha).

Adult rat brain immunohistochemistry.

Adult rat brain immunohistochemistry.

Immunoblot against adult rat brain membranes (RBM) and adult mouse hippocampal membranes (MHM) from PSD-95 and -93 knockout (KO) mice probed with K28/43 (left) or K28/86 (right) TC supe. Mouse samples courtesy of Richard Huganir (Johns Hopkins University, Howard Hughes Medical Institute).

Immunoblot against adult rat brain membranes (RBM) and adult mouse hippocampal membranes (MHM) from PSD-95 and -93 knockout (KO) mice probed with K28/43 (left) or K28/86 (right) TC supe. Mouse samples courtesy of Richard Huganir (Johns Hopkins University, Howard Hughes Medical Institute).

Immunofluorescence staining of cultured rat hippocampal neurons with K28/43 (green) and K57/1 (red, Kv4.2), right image is higher magnification of left image (dotted lines).
Electron micrograph of K28/43 hippocampal labelling using a post-embedding immunogold method. Immunoparticles (arrows) are seen in the postsynaptic densities of dendritic spines (s) forming asymmetrical synapses with axon terminals (b). Scale bar = 200 nm. Image courtesy of Rafael Lujan (Universidad de Castilla-La Mancha).
Adult rat brain immunohistochemistry.
Immunoblot against adult rat brain membranes (RBM) and adult mouse hippocampal membranes (MHM) from PSD-95 and -93 knockout (KO) mice probed with K28/43 (left) or K28/86 (right) TC supe. Mouse samples courtesy of Richard Huganir (Johns Hopkins University, Howard Hughes Medical Institute).

from NeuroMab

PSD-95 (7x-028)

  • Sale
  • $325

↓ Product Details | ↓ Citations


Volume: Purified: 100 µL; Purified Trial: 20 µL; TC Supernatant: 5 mL

Concentration: Purified: 1 mg/mL; TC Supernatant: Lot Specific

Clonality: Monoclonal

Form: Purified and TC Supernatant Available (select your preferred form, size and quantity before clicking "Add to Cart")

Host Species: Mouse

Immunogen: Fusion protein amino acids 77-299 (PDZ domains 1 and 2) of human PSD-95 (also known as Postsynaptic density protein 95, Disks large homolog 4, Synapse-associated protein 90, DLG4, Dlgh4 and SAP-90, accession number P78352)
Rat: 100% identity (223/223 amino acids identical)
Mouse: 99% identity (221/223 amino acids identical)
65-80% identity with DLG1/SAP97, DLG2/Chapsyn-110 and DLG3/SAP102

Target Description: PSD-95 is a member of the MAGUK (membrane associated guanylate kinase) family (which also includes PSD-93, SAP97 and SAP102) and functions as a scaffold protein that can cluster membrane receptors, ion channels, including NMDA receptor subunits, Kv1 potassium channels and Caspr2. The protein is encoded by DLG4, contains hallmark PDZ, SH3 and GuK domains and is found at post synaptic densities where it plays an important role in the formation and maintenance of synaptic junctions (Zhou and Blumberg, 2003). Indeed, overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. Antibodies against PSD95/DLG4 are frequently used in conjunction with pre-synaptic markers to localize synapses by microscopy

Gene ID: DLG4 PSD95

Antibody Registry ID (RRID): AB_2292909

Physical State: Liquid

Validation and Application Notes

Molecular Weight: 95-110 kDa(varies with cell background due to phosphorylation)

NOTE

Aves Labs products are intended for use as research laboratory reagents. They are not intended for use as diagnostic or therapeutic reagents in humans.


↑ back to top

Citations


↑ back to top

Back to New Products