
Immunostaining of granule cells in the dentate gyrus of the hippocampus from saline treated mouse brain staining ERK/MAPK when phosphorylated at Thr202/Tyr204 (cat#p160-2024, 1:500, red). The blue is staining nuclei with DAPI. The MAPK positive neurons show punctate staining primarily localized in the nucleus with few staining both cytoplasmic and nuclear. Photo courtesy of Robert Wine.
We are the original manufacturer of our ERK/MAPK (Thr202, Tyr204) rabbit polyclonal phosphospecific antibody, affinity purified from pooled serum. Optimized in WB and IHC.
Volume: 100 µL (Standard), 25 µL (Trial Size)
Concentration: Lot Specific
Clonality: Polyclonal
Form: Antigen Affinity Purified from Pooled Serum
Host Species: Rabbit
Species Reactivity: Human, Mouse, Rat
Applications: WB, IHC
Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr202/Tyr204 conjugated to KLH
Protein Name / Synonyms: ERK1/2 Thr202/Tyr204, ERK1/2 phospho, pERK, phospho ERK, ERK 1/2
Target Description: Extracellular-Signal Regulated Kinase/Mitogen-Activated Protein Kinase (ERK/MAPK) is an integral component of cellular signaling during mitogenesis and differentiation of mitotic cells and also is thought to play a key role in learning and memory (Adams and Sweatt, 2002; Ahn, 1993; Tanoue and Nishida, 2003; Johnson and Lapadat, 2002). The activity of this kinase is regulated by dual phosphorylation at Thr202 and Tyr204 (Ahn, 1993).
Gene ID: MAPK1/2
Antibody Registry ID (RRID): AB_2492090
Physical State: Liquid
Conjugate: Unconjugated
Buffer: 100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
Validation and Application Notes
Molecular Weight: 42/44
Storage
-20 C
NOTE
Aves Labs products are intended for use as research laboratory reagents. They are not intended for use as diagnostic or therapeutic reagents in humans.