Western blot of rat hippocampal lysate showing specific immunolabeling of the ~100 kDa GluR1 protein phosphorylated at Ser845 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 min).
from PhosphoSolutions
We are the original manufacturer of our GluR1 (Ser845) rabbit polyclonal phosphospecific antibody, affinity purified from pooled serum. Optimized in WB and IHC.
Volume: 100 µL (Standard), 25 µL (Trial Size)
Concentration: Lot Specific
Clonality: Polyclonal
Form: Antigen Affinity Purified from Pooled Serum
Host Species: Rabbit
Species Reactivity: Mouse, Rat
Applications: WB, IHC
Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser845 conjugated to KLH
Protein Name / Synonyms: GluA1, Anti GluR, Anti GluR A, pglur, pampa, pglua1, s845, ser845
Target Description: The ion channels activated by glutamate are typically divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by a-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as AMPA receptors (AMPAR). The AMPAR are comprised of four distinct glutamate receptor subunits designated (GluR1-4) and they play key roles in virtually all excitatory neurotransmission in the brain (Keinänen et al., 1990; Hollmann and Heinemann, 1994). The GluR1 subunit is widely expressed throughout the nervous system. Phosphorylation of Ser-845 on GluR1 is thought to be mediated by PKA and phosphorylation of this site increases the conductance of the AMPAR (Roche et al., 1996; Banke et al., 2000). In addition, phosphorylation of this site has been linked to synaptic plasticity as well as learning and memory (Lee at al., 2003; Esteban at al., 2003).
Gene ID: GRIA1
Antibody Registry ID (RRID): AB_2492128
Physical State: Liquid
Conjugate: Unconjugated
Buffer: 100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
Validation and Application Notes
Molecular Weight: 100
Storage
-20 C
NOTE
Aves Labs products are intended for use as research laboratory reagents. They are not intended for use as diagnostic or therapeutic reagents in humans.