Immunostaining of light-stimulated rabbit retina showing labeling of TH when phosphorylated at Ser40.

Immunostaining of light-stimulated rabbit retina showing labeling of TH when phosphorylated at Ser40.

Western blot of recombinant phospho-TH and non-phospho-TH showing selective immunolabeling by the phosphospecific antibody of the ~60 kDa TH phosphorylated at Ser40. The pan-specific antibody (anti-pan-TH) recognized both the phospho- and non-phospho-TH; while most importantly, the phospho-specific antibody (anti-Ser40 TH) recognized only phospho-TH.

Western blot of recombinant phospho-TH and non-phospho-TH showing selective immunolabeling by the phosphospecific antibody of the ~60 kDa TH phosphorylated at Ser40. The pan-specific antibody (anti-pan-TH) recognized both the phospho- and non-phospho-TH; while most importantly, the phospho-specific antibody (anti-Ser40 TH) recognized only phospho-TH.

from PhosphoSolutions

Anti-Tyrosine Hydroxylase (Ser40) (p1580-40)

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Product Details


We are the original manufacturer of our Tyrosine Hydroxylase (Ser40) rabbit polyclonal phosphospecific antibody. It is affinity purified from pooled serum, and great in WB, IHC, and ICC.


Volume: 100 µL (Standard), 25 µL (Trial Size)

Concentration: Lot Specific

Clonality: Polyclonal

Form: Antigen Affinity Purified from Pooled Serum

Host Species: Rabbit

Species Reactivity: Many Mammalian, many Non-mammalian

Applications: WB, IF, IHC

Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser40 conjugated to KLH

Protein Name / Synonyms: anti tyrosine hydroxylase ser40, anti TH ser40, ser40, anti tyrosine hydroxylase, anti-tyrosine hydroxylase, ptyrosine, phospho th, anti-th, hydoxylase

Target Description: Tyrosine hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines Dopamine and Norepinephrine. TH antibodies can therefore be used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson’s disease and drug abuse (Kish et al., 2001; Zhu et al., 2000; Zhu et al., 1999). TH antibodies can also be used to explore basic mechanisms of dopamine and norepinephrine signaling (Witkovsky et al., 2000; Salvatore et al., 2001; Dunkley et al., 2004). The activity of TH is also regulated by phosphorylation (Haycock et al., 1982; Haycock et al., 1992; Jedynak et al., 2002). Phospho-specific antibodies for the phosphorylation sites on TH can be used to great effect in studying this regulation and in identifying the cells in which TH phosphorylation occurs.

Gene ID: TH

Antibody Registry ID (RRID): AB_2492279

Physical State: Liquid

Conjugate: Unconjugated

Buffer: 100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Validation and Application Notes

Molecular Weight: 60

Storage

-20 C

NOTE

Aves Labs products are intended for use as research laboratory reagents. They are not intended for use as diagnostic or therapeutic reagents in humans.


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