Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). For longer storage periods, dilute the antibodies with sterile glycerol and store at -20°C. Do not freeze these antibodies unless you want to store them for longer periods of time.
Goats were immunized with purified chicken IgY (whole protein) emulsified in Freund’s adjuvant. After multiple injections, goats were bled and serum collected. Antibodies were isolated using affinity chromatography and conjugated to alkaline phosphatase.
HEPES (50 mM, pH 7.0), NaCl (0.1 M), bovine serum albumin (0.2%, w/v), MgCl2 (1.0 mM), ZnCl2 (0.1 mM), sodium azide (0.02%, w/v) (as an antimicrobial agent).
Does not cross-react with mammalian IgGs
Antibodies were analyzed using immunoelectrophoresis (see below) and a sandwich ELISA. In this latter test, a 1:800 dilution of GGHL-30PH-2 was found to generate an O.D of 1.0 in a 20 minute reaction with p-nitrophenyl phosphate as the substrate.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
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