Our anti-actin antibody (made against rabbit muscle actin) recognizes mouse, rat and human actin proteins in SDS-polyacrylamide gel transfers onto nitrocellulose membranes. This loading control assures that similar amounts of total proteins were added to each of the lanes in the gels. Note that this product does not work in transfers using PVDF membranes.
β-Actin Loading Control for Westerns using Nitrocellulose Membranes; 3 Lanes loaded with mouse muscle homogenate (5 ug total protein, 10 ug total protein, 20 ug total protein); 1' antibody -- ACT (1:1000); 2' antibody -- HRP Goat anti-chicken IgY (Aves Labs, 1:2000)
Store at -20˚C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). Do not freeze antibodies at -80˚C, unless you want to store them for longer periods of time. Note that each time an antibody preparation is frozen about half its binding activity is lost.
Chickens were immunized with Actin purified from rabbit muscle. After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column. The final product contains 50% glycerol (v/v), purified anti-Actin IgY fraction (10 mg/ml) spiked with 25 µg/ml of affinity purified anti-Actin IgY, and then filter-sterilized.
Sodium phosphate (10 mM, pH 7.2) buffered isotonic saline (0.9%, w/v), glycerol (50%, v/v), with sodium azide (0.02%, w/v) as an anti-microbial agent.
WB Brain: 1:1000-1:2000
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.