Chicken anti-SNCA (green) and TH (red) double immunostaining in Parkinson's Disease patient’s substantia nigra section. Arrows indicate SNCA positive, degenerating dopamine neurons. Arrowheads indicate SNCA negative, healthy dopamine neurons. Bar = 50 um for A-D. (Image courtesy of Dr. Curt Freed’s lab, University of Colorado Anschutz Medical Campus.)
Detect endogenous levels of alpha-synuclein (SNCA), also known as PARK1, NACP, PARK4, by Western Blot, immunohistochemistry, and immunofluorescence using this highly-validated chicken anti-alpha-synuclein antibody.
Volume: 100 µL
Concentration: 10 mg/mL
Form: IgY Fraction
Host Species: Chicken
Species Reactivity: Human, Mouse, Rat
Applications: IHC, ICC, WB, IF
Immunogen: Full-length human alpha-synuclein (UniProtKB - P37840) produced in E.Coli.
Protein Name / Synonyms: Alpha-synuclein, Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor, NACP
Target Description: Alpha-synuclein (SNCA), also known as PARK1, NACP, PARK4, is a member of the synuclein family, which also includes beta- and gamma-synuclein. Alpha-synuclein is a highly-conserved protein known to be abundant in neurons and especially presynaptic terminals where it serves multiple roles including regulation of synaptic vesicle trafficking and subsequent neurotransmitter release. Functionally, alpha synuclein has been implicated in synaptic plasticity (Liu 2004) and the assembly of snare complexes (Burre 2010) and it is known to be associated with various neuropathologies including Parkinson's Disease, Lewy Body Dementia, and Alzheimer's Disease, where it forms insoluble protein aggregates. Early studies of amyloid deposits in the brains of Alzheimer's patients revealed two previously uncharacterized peptides in addition to the amyloid beta fragment (Ueda 1993), both of which were found to correspond to alpha-synuclein (SNCA). Later studies confirmed that aggregated alpha-synuclein proteins are present in brain lesions (Lewy bodies) that are hallmarks of neurodegenerative synucleinopathies and that alpha synuclein likely plays a role in the pathogenesis of Parkinson's disease, Lewy body dementia, and Alzheimer’s disease among other neuropathologies. The SNCA antibody was raised against full-length human alpha synuclein and recognizes endogenous levels of alpha synuclein in brain by Western blot and immunohistochemistry.
Gene ID: SNCA, NACP, PARK1
Physical State: Liquid
Buffer: Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline ("PBS,” pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.
Production Notes: Chickens were immunized with recombinant human α-synuclein protein (expressed in bacteria). After repeated injections, immune eggs were collected, the IgY fractions were purified from the yolks, and the IgY concentration adjusted to 10 mg/ml. Finally, the antibody preparation was filter-sterilized.
Validation and Application Notes
Molecular Weight: 14 kDa
Western Blot Dilution Range: 1:1000-1:5000
IHC Dilution Range: 1:100-1:500
Antibodies were analyzed by western blot analysis (1:2500 dilution) on rat brain lysate and immunohistochemistry (1:200 dilution) on substantia nigra sections from a Parkinson’s Disease patient's brain. Western blots were performed using BlokHen® (Aves Labs) as the blocking reagent, and HRP-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# H-1004) as the detection reagent. Immunohistochemistry used FITC-conjugated goat anti-chicken IgY (AvesLabs Cat. # F-1005).
Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. Do not freeze these antibodies unless you want to store them for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half of its binding activity is lost.
Aves Labs products are intended for use as research laboratory reagents. They are not intended for use as diagnostic or therapeutic reagents in humans.