Western blot of rat cortical lysate showing specific immunolabeling of the ~15 kDa alpha synuclein protein phosphorylated at Ser129 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding non-phosphopeptide (not shown).
Details
Alpha-synuclein is a presynaptic neuronal protein that is thought to be involved in the formation of SNARE complexes. Most significantly, aggregated alphasynuclein is one of the major components found in the Lewy bodies that occur in Parkinson’s disease (PD) and other neurodegenerative disorders (Okochi et al., 2000). Mutation of the alpha-synuclein gene is associated with familial forms of PD (Newmann et al., 2002). Recent evidence suggests that phosphorylation of alpha-synuclein at Ser129 plays a role in the formation of inclusions in PD (Smith et al., 2005; Masliah et al., 2005).Volume: 100 µL
Concentration: Lot Specific
Clonality: Polyclonal
Form: Antigen Affinity Purified from Pooled Serum
Host Species: Rabbit
Species Reactivity: Human, Mouse, Rat
Applications: WB
Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser129 conjugated to KLH
Protein Name / Synonyms: a-synuclein, phospho alpha synuclein, ser129, 129
Gene ID: SNCA
Antibody Registry ID (RRID): AB_2492038
Physical State: Liquid
Conjugate: Unconjugated
Buffer: 100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
Validation and Application Notes
Molecular Weight: 15
Storage
-20 C
NOTE
Aves Labs products are intended for use as research laboratory reagents. They are not intended for use as diagnostic or therapeutic reagents in humans.