Western blot. Left Lane -- Cultured normal mouse embryo fibroblasts were transfected with a plasmid containing the SAP1a cDNA fused with a c-myc epitope tag at its C-terminus. Lane 1, 30 µg protein; lane 2, 20 µg protein; lane 3, 10 µg protein; lane 4, 5 µg protein.
Anti-C-MYC Epitope Tag Antibody
Bulk Order Anti-C-MYC Epitope Tag Antibody
C-MYC Epitope Tag Antibody
The synthetic peptide containing the human c-myc epitope (i.e., EQKLISEEDL).
Affinity Purified IgY
100 µg/mL (based on absorbance at 280 nm)
ELISA, ICC, IHC, IP, WB
Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.
Chickens were immunized with a synthetic peptide containing the human c-myc epitope (i.e., EQKLISEEDL) conjugated to keyhole limpet hemocyanin (KLH). After repeated injections, immune eggs were collected, the IgY fractions were purified from the yolks, and then affinity purified using a peptide column. The concentration of the eluate was then adjusted to 100 µg/mL, and the preparation was filter-sterilized.
Phosphate-buffered saline (PBS) (pH 7.2), sodium azide (0.02%, w/v) as an anti-microbial agent.
Antibodies were analyzed using ELISA and Western Blots using an N-terminal C-MYC-tagged protein. Secondary antibodies used in these quality control assays were alkaline phosphatase- (1:1000 dilution) and HRP-labeled (1:3000 dilution) goat anti-chicken IgY antibodies (Aves Labs), respectively.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
12 months from receipt of product
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