Our Anti-ChAT (Choline Acetyltransferase) primary antibody from PhosphoSolutions is goat polyclonal. It detects chicken, guinea pig, human, mouse, and non-human primate, and rat ChAT (Choline Acetyltransferase) and is affinity purified. It is great for use in WB, IHC.
Western blot of rat hippocampal lysate showing specific immunolabeling of the ~70 kDa ChAT.
Anti-ChAT (Choline Acetyltransferase) Antibody
ChAT (Choline Acetyltransferase)
Choline acetyltransferase is a neuronal enzyme which catalyzes the reaction between Acetyl CoA and choline resulting in the formation of acetylcholine. It is therefore found primarily in cholinergic neurons making it a valuable marker for diseases associated with decreased cholinergic function such as Schizophrenia, Alzheimer's disease (AD) and Down syndrome (Holt et al. 1999). Decreased choline acetyltransferase activity in particular has been shown in Schizophrenic subjects (Karson et al 1993). It has furthermore been demonstrated that in patients with AD, there are significantly lower levels of cortical ChAT that correlate with severity of the disease as measured by loss of neuropsychological function (Baskin et al. 1999).
Recombinant human choline acetyltransferase
Chicken, Guinea Pig, Human, Mouse, Non-Human Primate, Rat
Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.
Prepared from goat serum by affinity purification using a column to which the protein immunogen was coupled.
PBS + 5 mg/ml BSA + 0.2% NaN3
WB Brain: 1:100
Specific for endogenous levels of the ~70 kDa choline acetyltransferase protein.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.