Anti-DOPA Decarboxylase Antibody

Name: Anti-DOPA Decarboxylase Antibody
Brand: PhosphoSolutions
SKU: 417-DDC
Price: 430.0 USD
Availability: InStock

1 Citation

Our Anti-DOPA Decarboxylase rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects bovine, human, mouse, and rat DOPA Decarboxylase and is antigen affinity purified from pooled serum. It is great for use in WB.

Western blot of rat adrenal medulla showing specific immunolabeling of the ~55 kDa DDC protein.

Immunostaining of a frozen section of paraformaldehyde-fixed mouse brain showing specific immunolabeling of DDC (1:500) in red and fluorescent Nissl (blue). Photo courtesy of Tom Finger, University of Colorado School of Medicine.

Species Reactivity

Bovine, Human, Mouse, Rat

Applications

Host Species

Rabbit

Go to Full Product Details

from PhosphoSolutions Datasheet

Trial Size

Pooled Serum

SKU: 417-DDC

Volume: 100 µL

Variant

Price:

$430

Quantity:

Product Details

Target

DOPA Decarboxylase

Target Description

DOPA decarboxylase (aromatic L-amino acid decarboxylase, AADC; DDC) catalyzes the second reaction in the biosynthesis of catecholamines and serotonin (Waymire and Haycock, 2002; Berry et al., 1996; Haycock et al., 2003). It is also involved in the biosynthesis of trace amines. DDC antibodies can therefore be used as markers for dopaminergic, noradrenergic and serotonergic neurons in a variety of applications including depression, schizophrenia, Parkinson’s disease and drug abuse (Kish et al., 2001; Zhu et al., 2000; Zhu et al., 1999).

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

Host Species

Rabbit

Gene Name

DDC

Molecular Weight

55 kDa

Antigen

SDS denatured, recombinant bovine aromatic DOPA decarboxylase expressed in E. coli and purified from inclusion bodies.

Species Reactivity

Bovine, Human, Mouse, Rat

Antibody Registry ID

AB_2492072

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled rabbit serum by affinity purification using an AminoLink® Plus matrix to which purified, recombinant bovine DDC was coupled.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000
WB Brain: 1:1000

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~55 kDa DDC protein.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

AADC antibody, Aromatic L Amino Acid Decarboxylase antibody, Aromatic-L-amino-acid decarboxylase antibody, DDC antibody, DDC_HUMAN antibody, DOPA decarboxylase (aromatic L-amino acid decarboxylase) antibody, DOPA decarboxylase antibody

UniProt Details

UniProt (Immunogen Species): P27718

NCBI Gene ID

280762

Product Specific References for Applications and Species

Western Blot: Human

Western Blot: Human
PMID	Dilution	Publication
14535941	1.2ug/ml	Haycock, J.W., et al. 2003. Marked disparity between age‐related changes in dopamine and other presynaptic dopaminergic markers in human striatum. Journal of Neurochemistry, 87(3), pp.574-585.

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Product Specific Protocols

Western Blot Protocol: Download

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