Anti-DYKDDDDK Epitope Tag/FLAG Antibody

Name: Anti-DYKDDDDK Epitope Tag/FLAG Antibody
Brand: Aves Labs
SKU: ET-DY100
Price: 285.0 USD
Availability: InStock

from Aves Labs Datasheet

Product Type

Primary Antibody

Applications

ELISA, ICC, IHC, IP, WB

Host Species

Chicken

Format

Affinity-Purified IgY

Molecular Weight

N/A

Go to Full Product Details

Western blot. Right Lane -- Cultured HEK cells were transfected with a plasmid containing the RDF1 cDNA fused with a FLAG epitope tag at its C-terminus. Left Lane -- Cultured HEK cells with an empty plasmid vector. 25 μg of protein loaded in each lane.

Volume: 1 mL

Variant

Price:

$285

Quantity:

Bulk Order Anti-DYKDDDDK Epitope Tag/FLAG Antibody

Product Details

Target

DYKDDDDK Epitope Tag/FLAG Antibody

Format

Affinity-Purified IgY

Concentration

100 µg/mL

Clonality

Polyclonal

Isotype

IgY

Applications

ELISA, ICC, IHC, IP, WB

Host Species

Chicken

Molecular Weight

N/A

Antigen

Synthetic peptide

Antibody Registry ID

AB_2313510

Storage

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.

Physical State

Liquid

Production Notes

Chickens were immunized with a synthetic peptide containing the DYKDDDDK epitope conjugated to keyhole limpet hemocyanin (KLH). After repeated injections, immune eggs were collected, the IgY fractions were purified from the yolks, and then affinity purified using a peptide column. The concentration of the eluate was then adjusted to 100 μg/mL, and the preparation was filter-sterilized.

Buffer

Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS," pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.

Dilution Ranges

WB: 1:1000-1:2000
IHC: 1:500-1:1000
ICC: 1:500-1:1000

Quality Control

Antibodies were analyzed using ELISA and western blots using an N-terminal DYKDDDDK-tagged protein. Secondary antibodies used in these quality control assays was an HRP-labeled (1:5000 dilution) goat anti-chicken IgY antibodies (Aves Labs).

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

12 months from receipt of product

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Anti-DYKDDDDK Epitope Tag/FLAG Antibody