Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). For longer storage periods, dilute the antibodies with sterile glycerol and store at -20°C. Do not freeze these antibodies unless you want to store them for longer periods of time.
Chickens were immunized with a synthetic peptide containing the DYKDDDDK epitope conjugated to keyhole limpet hemocyanin (KLH). The first injection included complete Freund’s adjuvant; subsequent injections included incomplete Freund’s adjuvant. Eggs were collected after the fourth injection, and antibodies were purified from the yolks using a proprietary method that yields a purity of >90%. Antibodies were then affinity-purified over a peptide column and dialyzed against phosphate-buffered isotonic saline. Sodium azide was added, the protein concentration was adjusted to 100 mg/mL, and the antibodies were filter-sterilized (0.2 µm).
Antibodies were analyzed using ELISA and western blots using an N-terminal DYKDDDDK-tagged protein. Secondary antibodies used in these quality control assays was an HRP-labeled (1:5000 dilution) goat anti-chicken IgY antibodies (Aves Labs).
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
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