Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl– channel associated with the GABA-A receptor (GABA-A-R) subtype. GABA-A-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression and substance abuse. The GABA-A-R is a multimeric subunit complex. To date six as, four ßs and four ?s, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin,1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for a- and ß-subunits results in the expression of functional GABA-A-Rs sensitive to GABA. However, co-expression of a ?-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different a-subunits of the receptor (McKernan et al., 2000; Mehta and Ticku, 1998; Ogris et al., 2004; Pöltl et al., 2003). More recently there have been a number of studies demonstrating that the d-subunit of the receptor may affect subunit assembly (Korpi et al., 2002) and may also confer differential sensitivity to neurosteroids and to ethanol (Wallner et al., 2003; Wohlfarth et al., 2002).
Volume: 100 µL
Concentration: Lot Specific
Form: Antigen Affinity Purified from Pooled Serum
Host Species: Rabbit
Species Reactivity: Mouse, Rat
Immunogen: Fusion protein from the N-terminus of the delta subunit
Protein Name / Synonyms: GABA, GABAA delta, 868-GDN, 868A-GDN, delta, GABRD, GABAA, 868gdn, gabaar, ps219y
Gene ID: GABRD
Antibody Registry ID (RRID): AB_2631037
Physical State: Liquid
Buffer: 100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
Validation and Application Notes
Molecular Weight: 50
Aves Labs products are intended for use as research laboratory reagents. They are not intended for use as diagnostic or therapeutic reagents in humans.