Anti-GFAP Antibody (N206A/8)
Adult WT and KO mouse hippocampus immunofluorescence with N206A/8 (green) and KC rabbit anti- Kv2.1 (red). Brain samples courtesy of Albee Messing, University of Wisconsin at Madison.
Human, Mouse, Rat
ICC, IHC, WB
Mouse
from NeuroMab
KO Validated
Trial Size
Product Specific References for Applications and Species
- Immunocytochemistry: Human | Mouse | Rat
- Immunohistochemistry: Drosophila | Guinea Pig | Human | Mouse | Non-Human Primate | Polar Bear | Rat
- Western Blot: Human | Mouse | Rat
- Additional Publications: Unspecified
| Immunocytochemistry: Human | ||
| PMID | Dilution | Publication |
| 35701597 | 1:1000 | Liu, D., et al. 2022. Impact of Schizophrenia GWAS Loci Converge Onto Distinct Pathways in Cortical Interneurons vs Glutamatergic Neurons During Development. Molecular Psychiatry, . |
| 33287888 | not listed | Mou, Y., et al. 2020. Impaired lipid metabolism in astrocytes underlies degeneration of cortical projection neurons in hereditary spastic paraplegia. Journal Acta Neuropathologica Communications, . |
| 33097921 | 1:1000 | Park, G.H., et al. 2020. Activated microglia cause metabolic disruptions in developmental cortical interneurons that persist in interneurons from individuals with schizophrenia. Nature Neuroscience, . |
| 33097921 | 1:1000 | Park, G.H., et al. 2020. Activated microglia cause metabolic disruptions in developmental cortical interneurons that persist in interneurons from individuals with schizophrenia. Nature Neuroscience, 1352-1364. |
| 32202516 | not listed | Boisvert, E.M., et al. 2020. A Static Self-Directed Method for Generating Brain Organoids from Human Embryonic Stem Cells. Journal of visualized experiments, 10.3791/60379. |
| 29950669 | 1:500 | Park, J., et al. 2018. A 3D human triculture system modeling neurodegeneration and neuroinflammation in Alzheimer's disease.. Nat Neurosci, 941-951. |
| 27110916 | 1:100 | Xue, Y., et al. 2016. Sequential regulatory loops as key gatekeepers for neuronal reprogramming in human cells.. Nature Neuroscience, 807-15. |
| 24419315 | 1:500 | Williams, E.C., et al. 2014. Mutant astrocytes differentiated from Rett syndrome patients-specific iPSCs have adverse effects on wild-type neurons.. Hum Mol Genet., 2968-80. |
| 23313552 | 1:100 | Xue, Y., et al. 2013. Direct conversion of fibroblasts to neurons by reprogramming PTB-regulated microRNA circuits.. Cell., 82-96. |
| Immunocytochemistry: Mouse | ||
| PMID | Dilution | Publication |
| 37620442 | 1:100 | Jorfi, M, et al. 2023. Infiltrating CD8+ T cells exacerbate Alzheimer's disease pathology in a 3D human neuroimmune axis model. Nature neuroscience, 1489-1504. |
| 34531281 | 1:1000 | Griggs, R.B., et al. 2021. The type 2 diabetes factor methylglyoxal mediates axon initial segment shortening and alters neuronal function at the cellular and network levels.. Eneuro, ENEURO.0201-21.2021. |
| 33767611 | 1:1000 | Liakath-Ali, K., et al. 2021. The Perils of Navigating Activity-Dependent Alternative Splicing of Neurexins.. Front Mol Neurosci, 659681. |
| 32639590 | 1:300 | Padmashri, R., et al. 2021. Modeling human‐specific interlaminar astrocytes in the mouse cerebral cortex. Journal of Comparative Neurology, . |
| 32783930 | 1:1000 | Garay, P.M., et al. 2020. RAI1 Regulates Activity-Dependent Nascent Transcription and Synaptic Scaling. Cell Reports, 108002. |
| 30680309 | 1:1000 | Chander, P., et al. 2019. Neuron-Specific Gene 2 (NSG2) Encodes an AMPA Receptor Interacting Protein That Modulates Excitatory Neurotransmission. eNeuro, ENEURO.0292-18.2018. |
| 26883496 | not listed | Zhang, W., et al. 2016. MiRNA-128 regulates the proliferation and neurogenesis of neural precursors by targeting PCM1 in the developing cortex.. Elife., . |
| Immunocytochemistry: Rat | ||
| PMID | Dilution | Publication |
| 31682945 | 1:300 | Dragić, M., et al. 2019. Two Distinct Hippocampal Astrocyte Morphotypes Reveal Subfield-Different Fate during Neurodegeneration Induced by Trimethyltin Intoxication. Neurosciecne, 38-54. |
| Immunohistochemistry: Drosophila | ||
| PMID | Dilution | Publication |
| 35105675 | 1:500 | Wang, L., et al. 2022. Anastasis drives senescence and non-cell autonomous neurodegeneration in the astrogliopathy Alexander disease.. Journal of Neuroscience, 2584-2597. |
| 29765022 | 1:500 | Wang, L., et al. 2018. Tissue and cellular rigidity and mechanosensitive signaling activation in Alexander disease. Nature communications, 1899. |
| Immunohistochemistry: Guinea Pig | ||
| PMID | Dilution | Publication |
| 34530852 | 1:200 | Proskocil, B., et al. 2021. TLR7 is expressed by support cells, but not sensory neurons, in ganglia.. Journal of NeuroInflamation, 209. |
| Immunohistochemistry: Human | ||
| PMID | Dilution | Publication |
| 36001074 | 2.5ug/ml | Rodríguez-Palma, EJ, et al. 2023. Activation of α 6 -containing GABA A Receptors Induces Antinociception Under Physiological and Pathological Conditions. Pain, 948-966. |
| 35105675 | 1:500 | Wang, L., et al. 2022. Anastasis drives senescence and non-cell autonomous neurodegeneration in the astrogliopathy Alexander disease.. Journal of Neuroscience, 2584-2597. |
| 30975982 | 1:1000 | Boisvert, E.M., et al. 2019. Minocycline mitigates the effect of neonatal hypoxic insult on human brain organoids. Cell death and disease, 325. |
| 25864570 | 1:500 | Ristić, A.J., et al. 2015. Hippocampal antioxidative system in mesial temporal lobe epilepsy.. Epilepsia., 789-99. |
| 29765022 | 1:500 | Wang, L., et al. 2018. Tissue and cellular rigidity and mechanosensitive signaling activation in Alexander disease.. Nature Communications, 1899. |
| Immunohistochemistry: Mouse | ||
| PMID | Dilution | Publication |
| 36634053 | 1:500 | Fischer, M., et al. 2023. Distribution and Inflammatory Cell Response to Intracranial Delivery of Radioluminescent Y2(SiO4)O:Ce Particles. PloS One, e0276819. |
| 36336067 | 1:100 | New, L.E., et al. 2022. GABAergic Regulation Of Cell Proliferation Within The Adult Mouse Spinal Cord. Neuropharmacology, 109326. |
| 36220889 | not listed | He, L., et al. 2022. C9orf72 Functions in the Nucleus to Regulate DNA Damage Repair. Cell Death and Differentiation, . |
| 35972937 | 1:250 | Pohlentz, M.S., et al. 2022. Characterisation of NLRP3 Pathway-Related Neuroinflammation in Temporal Lobe Epilepsy. PloS One, e0271995. |
| 34231259 | 1:500 | Simankova, A., et al. 2021. Ddx20, DEAD box helicase 20, is essential for the differentiation of oligodendrocyte and maintenance of myelin gene expression. Glia, 2559-2574. |
| 35121636 | 1:25 | Matsuura, K., et al. 2022. SIPA1L1/SPAR1 interacts with the neurabin family of proteins and is involved in GPCR signaling. The Journal of neuroscience : the official journal of the Society for Neuroscience, . |
| 34911751 | not listed | Kim, E.C., et al. 2021. Spontaneous seizure and memory loss in mice expressing an epileptic encephalopathy variant in the calmodulin-binding domain of Kv7.2. Proceedings of the National Academy of Sciences of the United States of America, . |
| 34617601 | 1:500 | Vaasjo, L.O., et al. 2021. Characterization and manipulation of Corticothalamic neurons in associative cortices using Syt6-Cre transgenic mice. Journal of Comparative Neurology, 44590. |
| 34231259 | 1:500 | Simankova, A., et al. 2021. Ddx20, DEAD box helicase 20, is essential for the differentiation of oligodendrocyte and maintenance of myelin gene expression. Glia, 2559-2574. |
| 32574593 | 1:1000 | Poulin, J.F., et al. 2020. PRISM: A Progenitor-Restricted Intersectional Fate Mapping Approach Redefines Forebrain Lineages. Developmental Cell, 740-753.e3.. |
| 32249896 | not listed | Sullivan, C.S., et al. 2020. Developmental Regulation of Basket Interneuron Synapses and Behavior through NCAM in Mouse Prefrontal Cortex. Cerebral Cortex, 4689-4707. |
| 32033960 | 1:500 | Siehr, M.S., et al. 2020. Arx expansion mutation perturbs cortical development by augmenting apoptosis without activating innate immunity in a mouse model of X-linked infantile spasms syndrome. Disease models and mechanisms, dmm042515. |
| 29765022 | 1:500 | Wang, L., et al. 2018. Tissue and cellular rigidity and mechanosensitive signaling activation in Alexander disease.. Nature Communications, 1899. |
| 29665166 | 1:300 | Most, D., et al. 2020. Silencing Synaptic MicroRNA-411 Reduces Voluntary Alcohol Consumption in Mice. Addiction Biology, 604-616. |
| 31597963 | 1:250 | Piatkevich, K.D., et al. 2019. Population imaging of neural activity in awake behaving mice.. Nature, 413-417. |
| 31207335 | 1:500 | olde Heuvel, F., et al. 2019. STAT6 mediates the effect of ethanol on neuroinflammatory response in TBI. Brain Behavior and Immunity, 228-246. |
| 30771034 | 1:50 | Gatto, R.G., et al. 2019. Detection of axonal degeneration in a mouse model of Huntington's disease: comparison between diffusion tensor imaging and anomalous diffusion metrics.. Magnetic Resonance Materials in Physics, Biology and Medicine, 461–471. |
| 29361558 | 1:2 (supe) | Fairchild, C.L., et al. 2018. RBX2 maintains final retinal cell position in a DAB1-dependent and -independent fashion. Development, dev155283. |
| 29343209 | 1:250 | Hansen, K.R., et al. 2018. Mild Blast Injury Produces Acute Changes in Basal Intracellular Calcium Levels and Activity Patterns in Mouse Hippocampal Neurons. Journal of Neurotrauma, 1523-1536. |
| 29306441 | 1:2000 | Du, Y., et al. 2018. Liposome-Assisted Metabolic Glycan Labeling With Cell and Tissue Selectivity. Methods in Enzymology, 321-353. |
| 29133177 | 1:100 | DeWalt, G.J., et al. 2017. Region-specific alterations in astrocyte and microglia morphology following exposure to blasts in the mouse hippocampus. Neuroscience Letters, 160-166. |
| 28614186 | 1:500 | Gandla, J., et al. 2017. miR-34c-5p functions as pronociceptive microRNA in cancer pain by targeting Cav2.3 containing calcium channels. Pain, 1765-1779. |
| 27759100 | 1:500 | Ehmsen, J.T., et al. 2016. The astrocytic transporter SLC7A10 (Asc-1) mediates glycinergic inhibition of spinal cord motor neurons.. Scientific reports, 35592. |
| 27699209 | not listed | Ueki, Y., et al. 2016. Loss of Ikbkap causes slow, progressive retinal degeneration in a mouse model of familial dysautonomia.. Eneuro, 5. |
| 27251629 | 1:100 | Fan, F., et al. 2016. Dynamin 1-and 3-mediated endocytosis is essential for the development of a large central synapse in vivo.. Jounal of Neuroscience, 6097-6115. |
| 27109181 | not listed | Marin, M.A., et al. 2016. Amyloid-β plaques disrupt axon initial segments. Experimental Neurology, 93-98. |
| 26843629 | 1:1000 | Wang, L., et al. 2016. An In Vivo Pharmacological Screen Identifies Cholinergic Signaling as a Therapeutic Target in Glial-Based Nervous System Disease.. J Neurosci., 1445-55. |
| 26799938 | 1:100 | Lee, S.A., et al. 2016. Gradient index microlens implanted in prefrontal cortex of mouse does not affect behavioral test performance over time. . PloS one, e0146533.. |
| 26516618 | not listed | Pacey, L.K., et al. 2015. Persistent astrocyte activation in the fragile X mouse cerebellum.. Brain Behav., e00400. |
| 26348152 | 1:2000 | Jiang, W., et al. 2015. An optimized method for high-titer lentivirus preparations without ultracentrifugation.. Sci Rep., 13875. |
| 26038197 | 1:100 | Corns, L.F., et al. 2015. Cholinergic Enhancement of Cell Proliferation in the Postnatal Neurogenic Niche of the Mammalian Spinal Cord.. Stem Cells., 2864-76. |
| 24445073 | 1:500 | Robles, C.F., et al. 2014. Effects of kappa opioid receptors on conditioned place aversion and social interaction in males and females.. Behav Brain Res., 84-93. |
| 24336719 | 1:2000 | Gunn, B.G., et al. 2013. Dysfunctional astrocytic and synaptic regulation of hypothalamic glutamatergic transmission in a mouse model of early-life adversity: relevance to neurosteroids and programming of the stress response.. J Neurosci., 19534-54. |
| 24259590 | 1:1000 | Hagemann, T.L., et al. 2013. Deficits in adult neurogenesis, contextual fear conditioning, and spatial learning in a Gfap mutant mouse model of Alexander disease.. J Neurosci., 18698-706. |
| 23840874 | 1:500 | Cho, S.I., et al. 2013. Mechanisms of hearing loss after blast injury to the ear. Plos One, e67618. |
| 23542689 | 1:500 | Kang, S.H., et al. 2013. Degeneration and impaired regeneration of gray matter oligodendrocytes in amyotrophic lateral sclerosis.. Nat Neurosci., 571–579. |
| 23459611 | 1:1000 | Huang, L., et al. 2013. Reciprocal connectivity between mitral cells and external plexiform layer interneurons in the mouse olfactory bulb.. Front Neural Circuits., . |
| 23459611 | 1:1000 | Huang, L., et al. 2013. Reciprocal connectivity between mitral cells and external plexiform layer interneurons in the mouse olfactory bulb.. Frontiers in neural circuits, 32. |
| 23122958 | 1:200 | Chen, R., et al. 2012. Monoacylglycerol lipase is a therapeutic target for Alzheimer''s disease.. Cell Rep., 1329-39. |
| 23100420 | 1:400 | Ma, S., et al. 2012. Ric-8a, a guanine nucleotide exchange factor for heterotrimeric G proteins, regulates bergmann glia-basement membrane adhesion during cerebellar foliation.. J Neurosci., 14979-93. |
| 23039175 | 1:200 | Lu, J., et al. 2012. Pain in experimental autoimmune encephalitis: a comparative study between different mouse models.. J Neuroinflammation., . |
| 21436042 | 1:1000 | Ramsey, A.J., et al. 2011. Impaired NMDA receptor transmission alters striatal synapses and DISC1 protein in an age-dependent manner.. Proc Natl Acad Sci U S A., 5795-800. |
| Immunohistochemistry: Non-Human Primate | ||
| PMID | Dilution | Publication |
| 31589838 | 1:100 | Peterson, S.M., et al. 2019. Bardet-Biedl Syndrome in rhesus macaques: A nonhuman primate model of retinitis pigmentosa. Experimental Eye Research, 107825. |
| Immunohistochemistry: Polar Bear | ||
| PMID | Dilution | Publication |
| 26313569 | 1:2000 | Prüss, H., et al. 2015. Anti-NMDA receptor encephalitis in the polar bear (Ursus maritimus) Knut. . Scientific reports, 12805. |
| Immunohistochemistry: Rat | ||
| PMID | Dilution | Publication |
| 36001074 | 2.5ug/ml | Rodríguez-Palma, EJ, et al. 2023. Activation of α 6 -containing GABA A Receptors Induces Antinociception Under Physiological and Pathological Conditions. Pain, 948-966. |
| 35105675 | 1:500 | Wang, L., et al. 2022. Anastasis drives senescence and non-cell autonomous neurodegeneration in the astrogliopathy Alexander disease.. Journal of Neuroscience, 2584-2597. |
| 32730865 | 1:1000 | Stanisavljević, A., et al. 2020. Fluoxetine modulates neuronal activity in stress-related limbic areas of adult rats subjected to the chronic social isolation. Brain Research Bulliten, 95-108. |
| 31682945 | 1:300 | Dragić, M., et al. 2019. Two Distinct Hippocampal Astrocyte Morphotypes Reveal Subfield-Different Fate during Neurodegeneration Induced by Trimethyltin Intoxication. Neurosciecne, 38-54. |
| 31624998 | 1:500 | Bozic, I., et al. 2019. The Potassium Channel Kv1.5 Expression Alters During Experimental Autoimmune Encephalomyelitis. Neurochemical Research, 2733-2745. |
| 30460639 | 1:400 | Dakic, T., et al. 2019. Food For Thought: Short-Term Fasting Upregulates Glucose Transporters in Neurons and Endothelial Cells, But Not in Astrocytes. Neurochemical Research, 388-399. |
| 29574670 | 1:500 | Bozic, I., et al. 2018. Voltage Gated Potassium Channel Kv1.3 Is Upregulated on Activated Astrocytes in Experimental Autoimmune Encephalomyelitis. Neurochemical Research, 1020-1034. |
| 29567092 | 1:250 | Logan, C.N., et al. 2018. Nucleus accumbens GLT-1a overexpression reduces glutamate efflux during reinstatement of cocaine-seeking but is not sufficient to attenuate reinstatement. Neuropharmacology, 297-307. |
| 29345029 | 1:1000 | Lütt, A., et al. 2018. High prevalence and functional effects of serum antineuronal antibodies in patients with gastrointestinal disorders. Gastroenterology and motility, e13292. |
| 29163045 | 1:500 | Jakovljevic, M., et al. 2017. Down-regulation of NTPDase2 and ADP-sensitive P2 Purinoceptors Correlate with Severity of Symptoms during Experimental Autoimmune Encephalomyelitis. Frontiers in Cellular Neuroscience, . |
| 28965011 | 1:500 | Djedović, N., et al. 2017. Anti-encephalitogenic effects of ethyl pyruvate are reflected in the central nervous system and the gut. Biomedicine & Pharmacotherapy, 78-85. |
| 28578430 | 1:500 | Lavrnja, I., et al. 2017. Expression profiles of cholesterol metabolism-related genes are altered during development of experimental autoimmune encephalomyelitis in the rat spinal cord. . Scientific reports, 2702. |
| 28544147 | 1:500 | Dakic, T.B., et al. 2017. Short-term fasting promotes insulin expression in rat hypothalamus. European Journal of Neuroscience, 1730-1737. |
| 28544147 | 1:500 | Dakic, T.B., et al. 2017. Short-term fasting promotes insulin expression in rat hypothalamus. European Journal of Neuroscience, 1730-1737. |
| 24860436 | 1:500 | Seifi, M., et al. 2014. Localization of NG2 immunoreactive neuroglia cells in the rat locus coeruleus and their plasticity in response to stress.. Frontiers in Neuroanatomy, . |
| 23025758 | not listed | Baalman, K.L., et al. 2013. Blast wave exposure impairs memory and decreases axon initial segment length.. Journal of Neurotrauma, 741-751. |
| 22675541 | not listed | Manning, C.F., et al. 2012. Benefits and pitfalls of secondary antibodies: why choosing the right secondary is of primary importance.. PLoS One., e38313. |
| Western Blot: Human | ||
| PMID | Dilution | Publication |
| 35511821 | not listed | Yang, A.W., et al. 2022. Effects of Alexander disease-associated mutations on the assembly and organization of GFAP intermediate filaments. Molecular biology of the cell, mbcE22010013. |
| 29634780 | 1:1000 | Keren-Aviram, G., et al. 2018. Proteomic analysis of human epileptic neocortex predicts vascular and glial changes in epileptic regions. PLOS One, e0195639. |
| 28111074 | not listed | Huang, Y.W.A., et al. 2017. ApoE2, ApoE3, and ApoE4 differentially stimulate APP transcription and Aβ secretion. . Cell, 427-441. |
| 25864570 | 1:2000 | Ristić, A.J., et al. 2015. Hippocampal antioxidative system in mesial temporal lobe epilepsy.. Epilepsia., 789-99. |
| 29634780 | 1:1000 | Keren-Aviram, G., et al. 2018. Proteomic analysis of human epileptic neocortex predicts vascular and glial changes in epileptic regions.. Plos one, e0195639. |
| Western Blot: Mouse | ||
| PMID | Dilution | Publication |
| 35511821 | not listed | Yang, A.W., et al. 2022. Effects of Alexander disease-associated mutations on the assembly and organization of GFAP intermediate filaments. Molecular biology of the cell, mbcE22010013. |
| 32447612 | 1:10,000 | Gruol, D.L., et al. 2020. Alcohol alters IL-6 Signal Transduction in the CNS of Transgenic Mice with Increased Astrocyte Expression of IL-6. Cellular and Molecular Neuobiology, 733-750. |
| 31437534 | 1:10,000 | Roberts, A.J., et al. 2019. Increased IL-6 expression in astrocytes is associated with emotionality, alterations in central amygdala GABAergic transmission, and excitability during alcohol withdrawal. . Brain, Behavior, and Immunity., 188-202. |
| 29787738 | 1:10,000 | Gruol, D.L., et al. 2018. Altered Brain Activity During Withdrawal from Chronic Alcohol is Associated with Changes in IL-6 Signal Transduction and GABAergic Mechanisms in Transgenic Mice with Increased Astrocyte Expression of IL-6. Neuropharmacology., 32-46. |
| 28111074 | not listed | Huang, Y.W.A., et al. 2017. ApoE2, ApoE3, and ApoE4 Differentially Stimulate APP Transcription and Aβ Secretion. Cell, 427-441. |
| 24259518 | 1:2000 | Dagley, L.F., et al. 2014. Quantitative proteomic profiling reveals novel region-specific markers in the adult mouse brain.. Proteomics., 241-261. |
| Western Blot: Rat | ||
| PMID | Dilution | Publication |
| 34485605 | 1:1000 | Hooper, A.W., et al. 2021. Gene therapy using an ortholog of human fragile X mental retardation protein partially rescues behavioral abnormalities and EEG activity.. Molecular Therapy-Methods & Clinical Development, 196-209. |
| 26241743 | 1:6000 | Galbavy, W., et al. 2015. Neuroimmune and Neuropathic Responses of Spinal Cord and Dorsal Root Ganglia in Middle Age.. PLoS One., e0134394. |
| 24639151 | 1:500 | Hamity, M.V., et al. 2014. Increased neuronal expression of neurokinin-1 receptor and stimulus-evoked internalization of the receptor in the rostral ventromedial medulla of the rat after peripheral inflammatory injury.. J Comp Neurol., 3037-51. |
| Additional Publications: Unspecified | ||
| PMID | Publication | |
| 25393884 | Savic, D., et al. 2014. Ribavirin shows immunomodulatory effects on activated microglia.. Immunopharmacol Immunotoxicol., 433-41. | |
| 33908669 | Lin, N.H., et al. 2021. Elevated GFAP isoform expression promotes protein aggregation and compromises astrocyte function.. The FASEB Journal, e21614. | |