Anti-GluN2B/NR2B Glutamate Receptor Antibody (N59/36)
Primary Antibody
Human, Mouse, Rat
N59/36
IHC, IP, WB
Mouse
Purified
Grin2b
166 kDa
![Immunoblots on brain membranes prepared from whole rat (RBM) and mouse (MBM) brain, and from human cerebral cortex [HBM(Cx)] and hippocampus [HBM(H)].](http://www.aveslabs.com/cdn/shop/products/validation-image-73-101-n59-36-wb-2_{width}x.jpg?v=1626900672)
Cultured rat hippocampal neuron immunofluorescence. Image courtesy of Anthone Dunah (Harvard) and Morgan Sheng (MIT).
![Immunoblots on brain membranes prepared from whole rat (RBM) and mouse (MBM) brain, and from human cerebral cortex [HBM(Cx)] and hippocampus [HBM(H)].](http://www.aveslabs.com/cdn/shop/products/validation-image-73-101-n59-36-wb-2_1024x.jpg?v=1626900672)
Product Specific References for Applications and Species
- Immunocytochemistry: Mouse | Rat
- Immunohistochemistry: Mouse | Rat
- Western Blot: Human | Mouse | Rat
- Additional Publications: Unspecified
| Immunocytochemistry: Mouse | ||
| PMID | Dilution | Publication |
| 34962957 | 1:200 | Lee, G.S., et al. 2021. 14-3-3 proteins promote synaptic localization of N-methyl d-aspartate receptors (NMDARs) in mouse hippocampal and cortical neurons. PloS One, e0261791. |
| Immunocytochemistry: Rat | ||
| PMID | Dilution | Publication |
| 35177616 | 1:250 | Hruska, M., et al. 2022. Nanoscale rules governing the organization of glutamate receptors in spine synapses are subunit specific. Nature Communications, 920. |
| 34284042 | 1:1000 | Nuwer, J.L., et al. 2021. Sustained treatment with an α5 GABA A receptor negative allosteric modulator delays excitatory circuit development while maintaining GABAergic neurotransmission. Neuropharmacology, 108724. |
| Immunohistochemistry: Mouse | ||
| PMID | Dilution | Publication |
| 29464191 | 1:1000 | Galliano, E., et al. 2018. Impact of NMDA receptor overexpression on cerebellar purkinje cell activity and motor learning. eNeuro, ENEURO.0270-17. |
| Immunohistochemistry: Rat | ||
| PMID | Dilution | Publication |
| 32726631 | not listed | Holderith, N., et al. 2020. A High-Resolution Method for Quantitative Molecular Analysis of Functionally Characterized Individual Synapses. Cell Reports, 107968. |
| Western Blot: Human | ||
| PMID | Dilution | Publication |
| 34354080 | 1:500 | Tian, M., et al. 2021. GluN2A and GluN2B NMDA receptors use distinct allosteric routes. Nature Communications, 4709. |
| 32341572 | not listed | Jee, Y.H., et al. 2020. DLG2 variants in patients with pubertal disorders. Genetics in Medicine, 1329-1337. |
| Western Blot: Mouse | ||
| PMID | Dilution | Publication |
| 37567897 | 1:1000 | Eyford, BA, et al. 2023. Outside-in signaling through the major histocompatibility complex class-I cytoplasmic tail modulates glutamate receptor expression in neurons. Scientific reports, 13079. |
| 35842432 | 1:1000 | Focchi, E., et al. 2022. ATM Rules Neurodevelopment and Glutamatergic Transmission in the Hippocampus but not in the Cortex. Cell Death & Disease, 616. |
| 34962957 | 1:1000 | Lee, G.S., et al. 2021. 14-3-3 proteins promote synaptic localization of N-methyl d-aspartate receptors (NMDARs) in mouse hippocampal and cortical neurons. PloS One, e0261791. |
| 33428810 | not listed | Bae, M., et al. 2021. SLC6A20 transporter: a novel regulator of brain glycine homeostasis and NMDAR function. EMBO Molecular Medicine, e12632. |
| 33229412 | not listed | Pandian, S., et al. 2020. Myosin Va Brain-Specific Mutation Alters Mouse Behavior and Disrupts Hippocampal Synapses. eNeuro, ENEURO.0284-20.2020. |
| 33158444 | 1:1000 | Espinoza, S., et al. 2020. Neuronal surface P antigen (NSPA) modulates postsynaptic NMDAR stability through ubiquitination of tyrosine phosphatase PTPMEG. BMC Biology, 164. |
| 32639632 | 1:1000 | Ju, X., et al. 2020. Increasing the interval between repeated anesthetic exposures reduces long-lasting synaptic changes in late post-natal mice. Journal of Neurochemistry, 76-87. |
| 32147734 | 1:500 | Koch, N., et al. 2020. Syndapin I loss-of-function in mice leads to schizophrenia-like symptoms. Cerebral Cortex, 4306-4324. |
| 31200742 | 1:1000 | Micci, M.A., et al. 2019. Hippocampal stem cells promotes synaptic resistance to the dysfunctional impact of amyloid beta oligomers via secreted exosomes. Molecular Neurodegeneration, 25. |
| 30936304 | not listed | Won, S., et al. 2019. The STEP61 interactome reveals subunit-specific AMPA receptor binding and synaptic regulation. Proceedings of the National Academy of Science of the United States of America, 8028–8037. |
| 30451289 | 1:1000 | Vaden, J.H., et al. 2019. Chronic over-expression of ubiquitin impairs learning, reduces synaptic plasticity, and enhances GRIA receptor turnover in mice. Journal of Neurochemistry, 386-399. |
| 30288694 | 1:100 | Schroeder, E., et al. 2019. Neuron-type specific loss of CDKL5 leads to alterations in mTOR signaling and synaptic markers. Molecular Neurobiology, 4151-4162. |
| 30586380 | not listed | Favaro, P.D., et al. 2018. An opposing function of paralogs in balancing developmental synapse maturation. PloS Biology, e2006838. |
| 30200940 | 1:500 | Zhu, M., et al. 2018. Parkinson’s disease-linked Parkin mutations impair glutamatergic signaling in hippocampal neurons. BMC Biology, 100. |
| 29899451 | not listed | Wang, Z., et al. 2018. Quantitative phosphoproteomic analysis of the molecular substrates of sleep need. . Nature, 435-439. |
| 29118000 | not listed | Chowdhury, D., et al. 2018. Ca2+/calmodulin binding to PSD‐95 mediates homeostatic synaptic scaling down. The EMBO Journal, 122-138. |
| 28864826 | 1:500 | Tanabe, Y., et al. 2017. IgSF21 promotes differentiation of inhibitory synapses via binding to neurexin2α. Nature Communications, 408. |
| Western Blot: Rat | ||
| PMID | Dilution | Publication |
| 36014775 | 1:1000 | Tartaglione, A.M., et al. 2022. Short- and Long-Term Effects of Suboptimal Selenium Intake and Developmental Lead Exposure on Behavior and Hippocampal Glutamate Receptors in a Rat Model. Nutrients, 3269. |
| 27147118 | 1:3000 | Spencer, K.B., et al. 2016. FMRP mediates chronic ethanol‐induced changes in NMDA, Kv4. 2, and KChIP3 expression in the hippocampus. . Alcoholism: Clinical and Experimental Research, 1251-1261. |
| 34375626 | not listed | Moraga-Amaro, R., et al. 2021. Prenatal fluoxetine impairs non-hippocampal but not hippocampal memory in adult male rat offspring. Neuropharmacology, 108751. |
| 33373327 | 1:1000 | Pizzamiglio, L., et al. 2020. The DNA repair protein ATM as a target in autism spectrum disorder. JCI Insight, e133654. |
| 33185190 | 1:200 | Sanders, S.S., et al. 2020. The palmitoyl acyltransferase ZDHHC14 controls Kv1-family potassium channel clustering at the axon initial segment. eLife, e56058. |
| 33037073 | 1:1000 | Cochrane, V.A., et al. 2020. Leptin modulates pancreatic β-cell membrane potential through Src kinase–mediated phosphorylation of NMDA receptors. Journal of Biological Chemistry, 17281–17297. |
| 32349075 | 1:2000 | Zang, K.K., et al. 2020. Distinct Function of Estrogen Receptors in the Rodent Anterior Cingulate Cortex in Pain-related Aversion. Anesthesiology, 165-184. |
| 31996679 | 1:500 | Washburn, H.R., et al. 2020. Positive surface charge of GluN1 N-terminus mediates the direct interaction with EphB2 and NMDAR mobility. Nature Communications, 570. |
| 31801062 | 1:1000 | Paskus, J.D., et al. 2019. Synaptic Kalirin-7 and Trio Interactomes Reveal a GEF Protein-Dependent Neuroligin-1 Mechanism of Action. Cell Reports, 2944-2952.e5. |
| 30927362 | 1:500 | Durante, V., et al. 2019. Alpha-synuclein targets GluN2A NMDA receptor subunit causing striatal synaptic dysfunction and visuospatial memory alteration. Brain, 1365-1385. |
| 30396997 | 1:500 | Esmenjaud, J.B., et al. 2019. An inter-dimer allosteric switch controls NMDA receptor activity. The EMBO Journal, e99894. |
| 29487522 | 1:1000 | Uttl, L., et al. 2018. Chronic MK-801 application in adolescence and early adulthood: a spatial working memory deficit in adult Long-Evans rats but no changes in the …. Frontiers in Pharmacology, 42. |
| Additional Publications: Unspecified | ||
| PMID | Publication | |
| 28986874 | Tian, M., et al. 2017. Design of Light-Sensitive NMDARs by Genetically Encoded Photo-Cross-Linkers. Methods of Molecular Biology, 185-197. | |
| 27713495 | Tian, M., et al. 2016. Allosteric regulation in NMDA receptors revealed by the genetically encoded photo-cross-linkers. Scientific Reports, 34751. | |
| 27595347 | Clairfeuille, T., et al. 2016. A molecular code for endosomal recycling of phosphorylated cargos by the SNX27-retromer complex.. Nature Structural & Molecular Biology, 921-932. | |
| 27492494 | Jaramillo, T.C., et al. 2016. Novel Shank3 Mutant Exhibits Behaviors With Face Validity for Autism and Altered Striatal and Hippocampal Function. Autism Research, 42-65. | |
| 27480238 | Lie, E., et al. 2016. SALM4 suppresses excitatory synapse development by cis-inhibiting trans-synaptic SALM3-LAR adhesion.. Nature Communications, 12328. | |
| 27457929 | Won, S., et al. 2016. PSD-95 stabilizes NMDA receptors by inducing the degradation of STEP61.. Proceedings of the National Academy of Science of the United States of America, E4736-44. | |
| 27335408 | Xiao, X., et al. 2016. Disruption of Coordinated Presynaptic and Postsynaptic Maturation Underlies the Defects in Hippocampal Synapse Stability and Plasticity in Abl2/Arg-Deficient Mice. The Journal of Neuroscience, 6778–6791. | |
| 27216994 | Kaniakova, M., et al. 2016. Biochemical and electrophysiological characterization of N-glycans on NMDA receptor subunits. Journal of Neurochemistry, 546-556. | |
| 27161151 | Wang, X., et al. 2016. Altered mGluR5-Homer scaffolds and corticostriatal connectivity in a Shank3 complete knockout model of autism.. Nature Communications, 11459. | |
| 26930443 | Rambousek, L., et al. 2016. Rat intra-hippocampal NMDA infusion induces cell-specific damage and changes in expression of NMDA and GABAA receptor subunits. Neuropharmacology, 594-606. | |
| 26714288 | Moraga-Amaro, R., et al. 2016. Dopamine receptor D5 deficiency results in a selective reduction of hippocampal NMDA receptor subunit NR2B expression and impaired memory.. Neuropharmacology, 222-235. | |
| 26679993 | Stanic, J., et al. 2015. Rabphilin 3A retains NMDA receptors at synaptic sites through interaction with GluN2A/PSD-95 complex.. Nature Communications, 10181. | |
| 26665164 | Tao-Cheng, J.H., et al. 2015. Depolarization of hippocampal neurons induces formation of nonsynaptic NMDA receptor islands resembling nascent postsynaptic densities. Eneuro, ENEURO.0066-15. | |
| 26590557 | Tapia-Rojas, C., et al. 2015. Is L-methionine a trigger factor for Alzheimer''s-like neurodegeneration?: Changes in Aβ oligomers, tau phosphorylation, synaptic proteins, Wnt signaling and behavioral impairment in wild-type mice.. Molecular Neurodegeneration, 62. | |
| 26553645 | Park, H., et al. 2015. Mice lacking the PSD-95–interacting E3 ligase, Dorfin/Rnf19a, display reduced adult neurogenesis, enhanced long-term potentiation and impaired contextual …. Scientific reports, 16410. | |
| 26481044 | Stein, L.R., et al. 2015. Nampt is required for long-term depression and the function of GluN2B subunit-containing NMDA receptors.. Brain Research Bulletin, 41-51. | |
| 26479588 | Hruska, M., et al. 2015. Anchoring and synaptic stability of PSD-95 is driven by ephrin-B3.. Nature Neuroscience, 1594-1605. | |
| 26321637 | Li, Y., et al. 2015. Splicing-Dependent Trans-synaptic SALM3-LAR-RPTP Interactions Regulate Excitatory Synapse Development and Locomotion.. Cell Report, 1618-1630. | |
| 26241953 | Hanno-Iijima, Y., et al. 2015. Activity-Dependent Bidirectional Regulation of GAD Expression in a Homeostatic Fashion Is Mediated by BDNF-Dependent and Independent Pathways. PLoS One, e0134296. | |
| 25822337 | Balsara, R., et al. 2015. Conantokin-G attenuates detrimental effects of NMDAR hyperactivity in an ischemic rat model of stroke.. PLoS One, e0122840. | |
| 25771393 | Cristino, L., et al. 2015. d-Aspartate oxidase influences glutamatergic system homeostasis in mammalian brain.. Neurobiology of Aging, 1890-1902. | |
| 25653350 | Zhang, N., et al. 2015. S-SCAM, a rare copy number variation gene, induces schizophrenia-related endophenotypes in transgenic mouse model.. Journal of Neuroscience, 1892-1904. | |
| 25644714 | DiBattista, A.M., et al. 2015. Very low density lipoprotein receptor regulates dendritic spine formation in a RasGRF1/CaMKII dependent manner.. Biochimica et Biophysica Acta, 904-917. | |
| 25524173 | Serrano, F.G., et al. 2014. Andrographolide reduces cognitive impairment in young and mature AβPPswe/PS-1 mice.. Molecular Neurodegeneration, 61. | |
| 25510858 | Mulholland, P.J., et al. 2014. Neuroplasticity of A-type potassium channel complexes induced by chronic alcohol exposure enhances dendritic calcium transients in hippocampus.. Psychopharmacology, 1995-2006. | |
| 25505316 | Stroebel, D., et al. 2014. Controlling NMDA receptor subunit composition using ectopic retention signals.. Journal of Neuroscience, 16630-16636. | |
| 25471585 | Warmus, B.A., et al. 2014. Tau-mediated NMDA receptor impairment underlies dysfunction of a selectively vulnerable network in a mouse model of frontotemporal dementia.. Journal of Neuroscience, 16482-16495. | |
| 25232120 | Fitting, S., et al. 2014. Interactive HIV-1 Tat and morphine-induced synaptodendritic injury is triggered through focal disruptions in Na⁺ influx, mitochondrial instability, and Ca²⁺ overload.. Journal of Neuroscience, 12850-12864. | |
| 24941251 | Dai, S.H., et al. 2014. Activation of mGluR5 Attenuates NMDA-Induced Neurotoxicity through Disruption of the NMDAR-PSD-95 Complex and Preservation of Mitochondrial Function in Differentiated PC12 Cells. International Journal of Molecular Sciences, 10892-10907. | |
| 24874075 | Plowey, E.D., et al. 2014. Mutant LRRK2 enhances glutamatergic synapse activity and evokes excitotoxic dendrite degeneration.. Biochimica et Biophysica Acta, 1596-1603. | |
| 24501359 | Vargas, J.Y., et al. 2014. In vivo activation of Wnt signaling pathway enhances cognitive function of adult mice and reverses cognitive deficits in an Alzheimer''s disease model.. Journal of Neuroscience, 2191-2202. | |
| 24498267 | Landgraf, P., et al. 2014. Binding of Y-P30 to syndecan 2/3 regulates the nuclear localization of CASK.. PLoS One, e85924. | |
| 24486947 | Tronci, E., et al. 2014. Effect of memantine on L-DOPA-induced dyskinesia in the 6-OHDA-lesioned rat model of Parkinson''s disease.. Neuroscience, 245-252. | |
| 24440698 | Muñoz, F.J., et al. 2014. Wnt-5a increases NO and modulates NMDA receptor in rat hippocampal neurons.. Biochemical and Biophysical Research Communications, 189-194. | |
| 24360280 | Parkhurst, C.N., et al. 2013. Microglia promote learning-dependent synapse formation through brain-derived neurotrophic factor.. Cell, 1596-609. | |
| 24068818 | Krüger, J.M., et al. 2013. Differential roles of postsynaptic density-93 isoforms in regulating synaptic transmission.. Journal of Neuroscience, 15504-15517. | |
| 24012668 | Sohn, Y.I., et al. 2013. Antihypertensive drug Valsartan promotes dendritic spine density by altering AMPA receptor trafficking.. Biochemical and Biophysical Research Communications, 464-470. | |
| 23946397 | Bonnet, S.A., et al. 2013. Synaptic state-dependent functional interplay between postsynaptic density-95 and synapse-associated protein 102.. Journal of Neuroscience, 13398-13409. | |
| 23926248 | Prox, J., et al. 2013. Postnatal disruption of the disintegrin/metalloproteinase ADAM10 in brain causes epileptic seizures, learning deficits, altered spine morphology, and defective synaptic functions.. Journal of Neuroscience, 12915-12928. | |
| 23884936 | Zamzow, D.R., et al. 2013. An increase in the association of GluN2B containing NMDA receptors with membrane scaffolding proteins was related to memory declines during aging.. Journal of Neuroscience, 12300-12305. | |
| 23805233 | Csölle, C., et al. 2013. Neurochemical Changes in the Mouse Hippocampus Underlying the Antidepressant Effect of Genetic Deletion of P2X7 Receptors.. PLoS One, e66547. | |
| 23540692 | Li, L., et al. 2013. Hijacking the neuronal NMDAR signaling circuit to promote tumor growth and invasion.. Cell, 86-100. | |
| 23486974 | Murata, Y., et al. 2013. Postsynaptic density scaffold SAP102 regulates cortical synapse development through EphB and PAK signaling pathway.. Journal of Neuroscience, 5040-5052. | |
| 23103165 | Chen, B.S., et al. 2012. SAP102 mediates synaptic clearance of NMDA receptors.. Cell Report, 1120-1128. | |
| 22902837 | She, K., et al. 2012. Differential stimulus-dependent synaptic recruitment of CaMKIIα by intracellular determinants of GluN2B.. Molecular and Cellular Neuroscience, 68-78. | |
| 22869717 | Ardiles, A.O., et al. 2012. Postsynaptic dysfunction is associated with spatial and object recognition memory loss in a natural model of Alzheimer''s disease.. Proceedings of the National Academy of Science of the United States of America, 13835–13840. | |
| 22740692 | She, K., et al. 2012. Glutamate binding to the GluN2B subunit controls surface trafficking of N-methyl-D-aspartate (NMDA) receptors.. The Journal of Biological Chemistry, 27432–27445. | |
| 22593065 | Danielson, E., et al. 2012. S-SCAM/MAGI-2 is an essential synaptic scaffolding molecule for the GluA2-containing maintenance pool of AMPA receptors.. Journal of Neuroscience, 6967–6980. | |
| 22832522 | Inestrosa, N.C., et al. 2011. Tetrahydrohyperforin prevents cognitive deficit, Aβ deposition, tau phosphorylation and synaptotoxicity in the APPswe/PSEN1ΔE9 model of Alzheimer''s disease: a possible effect on APP processing.. Translational Psychiatry, e20. | |
| 22054645 | Marchand, F., et al. 2011. Specific involvement of atypical PKCζ/PKMζ in spinal persistent nociceptive processing following peripheral inflammation in rat.. Molecular Pain, 86. | |
| 21795692 | Verpelli, C., et al. 2011. Importance of Shank3 protein in regulating metabotropic glutamate receptor 5 (mGluR5) expression and signaling at synapses.. The Journal of Biological Chemistry, 34839–34850. | |
| 21762461 | Badanich, K.A., et al. 2011. NR2B-Deficient Mice are More Sensitive to the Locomotor Stimulant and Depressant Effects of Ethanol. Genes, Brain and Behavior, 805-816. | |
| 21496646 | Zhang, J., et al. 2011. The AAA+ ATPase Thorase regulates AMPA receptor-dependent synaptic plasticity and behavior.. Cell, 284-299. | |
| 21092855 | Micheva, K.D., et al. 2010. Single-synapse analysis of a diverse synapse population: proteomic imaging methods and markers.. Neuron, 639-653. | |
| 20678574 | Antion, M.D., et al. 2010. Ephrin-B3 regulates glutamate receptor signaling at hippocampal synapses.. Molecular and Cellular Neuroscience, 378-388. | |
| 19158293 | Gardoni, F., et al. 2009. Decreased NR2B subunit synaptic levels cause impaired long-term potentiation but not long-term depression.. The Journal of Neuroscience, 669-677. | |