Western blot of rat hippocampal lysate showing specific immunolabeling of the ~100 kDa GluR1 protein phosphorylated at Ser831 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 min).

Western blot of rat hippocampal lysate showing specific immunolabeling of the ~100 kDa GluR1 protein phosphorylated at Ser831 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 min).

from PhosphoSolutions

Anti-GluR1-Subunit (Ser831) (p1160-831)

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Product Details


Details

Volume: 100 µL (Standard), 25 µL (Trial Size)

Concentration: Lot Specific

Clonality: Polyclonal

Form: Antigen Affinity Purified from Pooled Serum

Host Species: Rabbit

Species Reactivity: Mouse, Rat

Applications: WB

Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser831 conjugated to KLH

Protein Name / Synonyms: GluA1, Anti GluR, Anti GluR A, pglur, pampa, pglua1, s831

Target Description: The ion channels activated by glutamate are typically divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by a-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as AMPA receptors (AMPAR). The AMPAR are comprised of four distinct glutamate receptor subunits designated (GluR1-4) and they play key roles in virtually all excitatory neurotransmission in the brain (Keinänen et al., 1990; Hollmann and Heinemann, 1994). The GluR1 subunit is widely expressed throughout the nervous system. GluR1 is potentiated by phosphorylation at Ser-831 which has been shown to be mediated by either PKC or CaM kinase II (McGlade-McCulloh et al., 1993; Mammen et al., 1999; Roche et al., 1996). In addition, phosphorylation of this site has been linked to synaptic plasticity as well as learning and memory (Soderling and Derkach, 2000).

Gene ID: GRIA1

Antibody Registry ID (RRID): AB_2492127

Physical State: Liquid

Conjugate: Unconjugated

Buffer: 100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Validation and Application Notes

Molecular Weight: 100

Storage

-20 C

NOTE

Aves Labs products are intended for use as research laboratory reagents. They are not intended for use as diagnostic or therapeutic reagents in humans.


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