Anti-Growth-Associated Protein-43 Antibody

Name: Anti-Growth-Associated Protein-43 Antibody
Brand: Aves Labs
SKU: GAP43
Price: 275.0 USD
Availability: InStock

from Aves Labs Datasheet

This antipeptide IgY antibody was generated in chickens against a C-terminal sequence shared between the mouse (NP_032109) and rat (NP_05889.1) gene products. It has been validated with human, mouse and rat tissues.

Product Type

Primary Antibody

Species Reactivity

Human, Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Chicken

Format

IgY Fraction

Gene Name

GAP43

Molecular Weight

43 kDa

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Mixed neuron-glial cultures stained with CPCA-GAP43 (green) and rabbit antibody to alpha-II spectrin (RPCA-aII-Spec, red), and DNA (blue). The GAP43 antibody stains numerous axonal and dendritic profiles in these cultures, clearly revealing the submembraneous cytoskeleton and vesicles. Photo by Dr. Gerry Shaw, Univ. Florida.

GAP-43 immunoreactivity in a neuroblast within a neurosphere culture. Chicken anti-GAP-43 antibody (1:500 dilution), Fluorescein-goat anti-chicken IgY secondary antibody (Aves Labs, 1:500). Photomicrograph by Hoda Ilias, Aves Labs.

Western blots of extract of cow hippocampus stained with CPCA-GAP43. A prominent band running at ~43 kDa represents GAP43. Photo courtesy of Dr. Gerry Shaw, Univ. Florida.

Mixed neuron-glial cultures stained with CPCA-GAP43 (green) and rabbit antibody to alpha-II spectrin (RPCA-aII-Spec, red), and DNA (blue). The GAP43 antibody stains numerous axonal and dendritic profiles in these cultures, clearly revealing the submembraneous cytoskeleton and vesicles. Photo by Dr. Gerry Shaw, Univ. Florida.

Volume: 200 µL

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Product Details

Target

Growth-Associated Protein-43

Target Description

Growth-Associated Protein-43 is a 274 amino acid cytoplasmic protein found in neurons and various other embryonic cell types. This protein also goes by a number of alternative names, including neuromodulin and F1. In cultured neurons, GAP-43 antibodies selectively stain the growth cones of axons. As the substrate for various phosphorylation events, it is believed that GAP-43 plays a critical role in axonal growth, although its exact function is still unclear.

Format

IgY Fraction

Concentration

2 mg/mL

Clonality

Polyclonal

Isotype

IgY

Applications

ICC, IHC, WB

Host Species

Chicken

Gene Name

GAP43

Molecular Weight

43 kDa

Antigen

Synthetic peptide

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2313546

Storage

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.

Physical State

Liquid

Production Notes

Chickens were immunized with a synthetic peptide corresponding to the C-terminal region of mouse GAP-43 coupled to keyhole limpet hemocyanin. After repeated injections, immune eggs were collected, the IgY fractions were purified from the yolks, and the IgY concentration adjusted to 20 mg/mL. This preparation was then diluted 1:10 with PBS containing bovine serum albumin as a carrier. Finally, the antibody preparation was filter-sterilized.

Buffer

Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline ("PBS" pH 7.2) with bovine serum albumin (BSA, 0.5%) added to prevent absorption to the plastic and sodium azide (0.02%, w/v) added as a preservative

Dilution Ranges

WB: 1:1000-1:2000
IHC: 1:500-1:1000
ICC: 1:500-1:1000

Quality Control

Quality assurance analysis was performed using immunohistochemistry (at a dilution of 1:1000) using fluorescein-labeled goat anti-chicken IgY (1:1000 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

12 months from receipt of product

Shipping

Ice packs

Synonyms

Neuromodulin (Axonal membrane protein GAP-43) (Growth-associated protein 43) (Neural phosphoprotein B-50) (pp46)

UniProt Details

UniProt (Immunogen Species): P17677

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Anti-Growth-Associated Protein-43 Antibody

This antipeptide IgY antibody was generated in chickens against a C-terminal sequence shared between the mouse (NP_032109) and rat (NP_05889.1) gene products. It has been validated with human, mouse and rat tissues.