Kv 2.1 K+ channel (Potassium voltage-gated channel subfamily B member 1), which is encoded by KCNB1 gene, is a member of the Potassium voltage-gated channel familly. This protein, mainly expressed in the central nervous system, functions as a delayed rectifier. Indeed, it regulates the action potential (AP) repolarization as well as duration and frequency of repetitive AP firing in neurons, muscle cells and endocrine cells. Kv2.1 K+ channel switches between open or closed conformation in response to the voltage difference across the membrane. This process directs the voltage-dependent potassium ion permeability of excitable membranes, which lets potassium ions pass in accordance with their electrochemical gradient. Kv2.1 K+ channel is widely used as a marker to measure membrane excitability in hippocampal neurons.
IHC, IP, WB
105-125 kDa (varies with cell background due to phosphorylation)
Synthetic peptide amino acids 837-853 (HMLPGGGAHGSTRDQSI, cytoplasmic Cterminus) of rat Kv2.1 (also known as Potassium voltage-gated channel subfamily B member 1, Delayed rectifier potassium channel 1, DRK1, Kcnb1 and Shab, accession number P15387); Mouse: 100% identity (17/17 amino acids identical); Human: 88% identity (15/17 amino acids identical, RVLPGGGAHGSTRDQSI)
Human, Mouse, Rat
Store at ≤ -20 C for long term storage. For short term storage, store at 2-8 C. For maximum recovery of product, centrifuge the vial prior to removing the cap.