Immunofluoresence of COS7 cells expressing mCherry using chicken anti-mCherry antibody (green) and showing mCherry autofluorescence (red). Blue is DAPI nuclear stain showing nuclei of both transfected and untransfected cells. Staining shows 100% correspondence between chicken anti-mCherry signal and mCherry autofluoresence in transfected cells.
Western blotting of recombinant fluorescent proteins with AvesLabs Chicken anti-mCherry antibody. The indicated (nanogram) amounts of each purified recombinant fluorescent protein was loaded on a gel and analyzed by Western blotting. AvesLabs Chicken anti-mCherry antibody recognizes ng amounts of mCherry protein and the related dsRED protein but does not show any reactivity with GFP.
Western blot of COS7 cells transfected with mCherry-myc-FLAG (lane 1), GFP (lane 2), or mock transfected cells (lane 3) using chicken α-mCherry antibody showing the expected staining for mCherry in lane 1 and no staining in GFP or untransfected cells. The ~37kDa major band in lane 1 represents full-length mCherry-myc-FLAG while the shorter ~24kDa band represents an mCherry degradation product.
Our anti-mCherry chicken primary antibody from AvesLabs is manufactured in-house. It detects mCherry, and is purified. It is provided in liquid form.
Volume: 100µL (MCHERRY-0020) or 500µL (MCHERRY-0100)
Host Species: Chicken
Antibody Isotype: IgY
Applications: ICC, IHC, WB
Target Description: mCherry is a red fluorescent protein derived from DsRed which is isolated from disc corals and related to GFP (green fluorescent protein) (Shaner et al., 2004). Fluorescent proteins are commonly used to tag proteins in order to localize their expression in both cultured cells and in vivo. Antibodies against fluorescent proteins such as mCherry are especially useful when amplification of the fluorescent signal is necessary or desired. Our chicken anti-mCherry antibody is a highly sensitive reagent for detection of mCherry by western blot, immunocytochemistry, and immunohistochemistry.
Gene ID: mCherry
Physical State: Liquid
Production Notes: Eggs from hens hyperimmunized with target were used to prepare an IgY fraction which was then subjected to antigen-specific affinity purification
Validation and Application Notes
Molecular Weight: 27 kDa for native monomer
Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. Do not freeze these antibodies unless you want to store them for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half of its binding activity is lost.
Aves Labs products are intended for use as research laboratory reagents. They are not intended for use as diagnostic or therapeutic reagents in humans.