Anti-Metabotropic Glutamate Receptor Type 2 / Type 3 (mGluR2/mGluR3) Antibody

Name: Anti-Metabotropic Glutamate Receptor Type 2 / Type 3 (mGluR2/mGluR3) Antibody
Brand: Aves Labs
SKU: ER2/3
Price: 440.0 USD
Availability: InStock

from Aves Labs Datasheet

We have three different anti-mGluR antibodies, each of which binds to a specific peptide sequence(s) present in different sets of mGluR gene family members. The ER2/3 antibody represents sequences found in both mGluR2 and mGluR3. (Note that this product cannot distinguish between these two receptors.) It comprises two antipeptide antibodies generated against overlapping sequences specific to the mGluR2 and mGluR3 gene products but not found in other mGluR gene family members. This antibody has been validated for immunohistochemistry and immunocytochemistry using mouse brain sections and cultured neuroblastoma cells as well as in rat brain sections.

Product Type

Primary Antibody

Species Reactivity

Human, Mouse, Rat

Applications

ICC, IHC

Host Species

Chicken

Format

Affinity-Purified IgY

Gene Name

GRM2 GPRC1B MGLUR2 (mGluR2) and GRM3 GPRC1C MGLUR3 (mGluR3)

Molecular Weight

99 kDa

Go to Full Product Details

Metabotropic glutamate receptor type 2 (mGluR2, 1:1000 dilution, green) immunoreactivity and neurofilament-M (NF-M, rabbit antibody, 1:500 dilution, red) immunoreactivity in a tissue section through an e18 mouse cochlear ganglion.

Metabotropic glutamate receptor type 3 (mGluR3, 1:1000 dilution, green) immunoreactivity and neurofilament-M (NF-M rabbit antibody, 1:500 dilution, red) immunoreactivity in a tissue section through the CA3 hippocampal formation of an e18 mouse embryo.

Metabotropic glutamate receptor type 2 (mGluR2, 1:1000 dilution, green) immunoreactivity and neurofilament-M (NF-M, rabbit antibody, 1:500 dilution, red) immunoreactivity in a tissue section through an e18 mouse cochlear ganglion.

Volume: 1000 µL

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$440

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Bulk Order Anti-Metabotropic Glutamate Receptor Type 2 / Type 3 (mGluR2/mGluR3) Antibody

Product Details

Target

Metabotropic Glutamate Receptor Type 2 / Type 3 (mGluR2 / mGluR3)

Target Description

The metabotropic glutamate receptors (mGluRs) represent a gene family of G-protein-coupled receptors, all of which bind the amino acid glutamate (the major excitatory neurotransmitter of the nervous system) but differ in their primary amino acid structure, their pharmacology, and their distribution in the peripheral and central nervous systems. In general, mGluR1 and mGluR5 receptors are post-synaptic and mGluR2 and mGluR3 receptors are pre-synaptic.

Format

Affinity-Purified IgY

Concentration

200 µg/mL

Clonality

Polyclonal

Isotype

IgY

Applications

ICC, IHC

Host Species

Chicken

Gene Name

GRM2 GPRC1B MGLUR2 (mGluR2) and GRM3 GPRC1C MGLUR3 (mGluR3)

Molecular Weight

99 kDa

Antigen

Synthetic peptides

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2313542

Storage

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.

Physical State

Liquid

Production Notes

Chickens were immunized with two synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. These synthetic peptides corresponded to unique sequences within the metabotropic glutamate receptor type 2 and type 3 gene products, but was shared between the human (Q14416.2, Q14832.2 NCBI) and mouse (Q14B12.2, Q9QYS2.1, NCBI) sequences. After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, the concentrations of the eluates adjusted to 200 µg/mL, and the preparation was filter-sterilized.

Buffer

Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS," pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.

Dilution Ranges

IHC: 1:1000-1:2000
ICC: 1:1000-1:2000

Quality Control

This anti-peptide antibody preparation was analyzed by immunohistochemistry (at a dilution of 1:2000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

12 months from receipt of product

Shipping

Ice packs

Synonyms

Metabotropic glutamate receptor 2 (mGluR2), Metabotropic glutamate receptor 3 (mGluR3)

UniProt Details

UniProt (Immunogen Species): Q14416

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Anti-Metabotropic Glutamate Receptor Type 2 / Type 3 (mGluR2/mGluR3) Antibody