Anti-Microtubule-Associated Protein Antibody

Name: Anti-Microtubule-Associated Protein Antibody
Brand: Aves Labs
SKU: MAP
Price: 425.0 USD
Availability: InStock

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Two antipeptide antibodies were generated in chickens against sequences shared between the mouse (P20357) and human (NP_002365) gene products. Antibodies were affinity-purified and mixed in equal concentrations. These antibodies have been validated with human, mouse, and rat tissues.

Product Type

Primary Antibody

Species Reactivity

Human, Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Chicken

Gene Name

MAP2

Molecular Weight

280 kDa

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Neonatal mouse brain cortical cultures were stained with Aves Labs MAP2 (green) and GFAP (red, rabbit antibody); Blue is DAPI nuclear stain. Photo courtesy of Dr. Gerry Shaw, Univ. Florida.

MAP-2 immunoreactivity in neurosphere cultures from an e18 mouse brain. MAP-2 is seen in GREEN (1:1000 dilution); 2˚ antibody was a fluorescein-labeled goat anti-chicken IgY (Aves Labs, 1:500 dilution). BLUE staining is DAPI nuclear stain. (Hoda Ilias, Aves Labs.)

Volume: 1000 µL

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Price:

$425

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Product Details

Target

Microtubule-Associated Protein (MAP2)

Target Description

Human MAP-2 is a 199,296 dalton protein (1827 amino acids) expressed in neurons of the PNS and CNS, where it serves as a major component of the neuronal cytoskeleton. MAP-2 contributes to structural integrity and cell shape.

Concentration

200 µg/mL

Clonality

Polyclonal

Isotype

IgY

Applications

ICC, IHC, WB

Host Species

Chicken

Gene Name

MAP2

Molecular Weight

280 kDa

Antigen

Synthetic peptides

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2313549

Storage

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). Do not freeze these antibodies unless you want to store them for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half of its binding activity is lost.

Physical State

Liquid

Production Notes

Chickens were immunized with two synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. These synthetic peptides corresponded to different regions of the MAP-2 gene product, but are shared between the human (NP_002365, NCBI) and mouse (P20357, NCBI) sequences. After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 200 µg/mL. Finally, equal volumes of both of these affinitypurified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.

Dilution Ranges

WB: 1:5000-1:10000
IHC: 1:1000-1:2000

Quality Control

Both of these anti-peptide antibodies were analyzed by immunohisto-chemistry (at a dilution of 1:2000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Shipping

Ice packs

Synonyms

Microtubule-associated protein 2 (MAP-2)

UniProt Details

UniProt (Immunogen Species): P11137

Shahsavani M, Pronk RJ, Falk R, Lam M, Moslem M, Linker SB, Salma J, Day K, Schuster J, Anderlid BM, Dahl N, Gage FH, Falk A. (2017), 'An in vitro model of lissencephaly: expanding the role of DCX during neurogenesis.' Molecular Psychiatry. 10.1038/mp.2017.175.
Zhang S, Zhao E, Winkelstein BA. (2017), 'A Nociceptive Role for Integrin Signaling in Pain After Mechanical Injury to the Spinal Facet Capsular Ligament.' Annals of Biomedical Engineering. 10.1007/s10439-017-1917-2.
Niere F, Raab-Graham KF. (2017), 'mTORC1 Is a Local, Postsynaptic Voltage Sensor Regulated by Positive and Negative Feedback Pathways.' Frontiers in Cellular Neuoroscience. 10.3389/fncel.2017.00152.
Li J, Han W, Pelkey KA, Duan J, Mao X, Wang YX, Craig MT, Dong L, Petralia RS, McBain CJ, Lu W. (2017), 'Molecular Dissection of Neuroligin 2 and Slitrk3 Reveals an Essential Framework for GABAergic Synapse Development.' Neuron. 10.1016/j.neuron.2017.10.003.
Ash PEA, Dhawan U, Boudeau S, Lei S, Carlomagno Y, Knobel M, Al Mohanna LFA, Boomhower SR, Newland MC, Sherr DH, Wolozin B. (2018), 'Heavy metal neurotoxicants induce ALS-linked TDP-43 pathology.' Toxicological Sciences. 10.1093/toxsci/kfy267.

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Anti-Microtubule-Associated Protein Antibody

Two antipeptide antibodies were generated in chickens against sequences shared between the mouse (P20357) and human (NP_002365) gene products. Antibodies were affinity-purified and mixed in equal concentrations. These antibodies have been validated with human, mouse, and rat tissues.