Immunostaining of PFA perfused frozen kidney sections from WT and NCC KO mice showing specific labeling of the NCC protein phosphorylated at Thr53 (cat #p1311-53, red, 1:100,000) on the top and the absence of staining in the KO on the left. (Image courtesy of Lauren Miller, Ellison Lab, OHSU.)
Western blot of mouse kidney lysate showing specific immunolabeling of the ~160 kDa NCC protein phosphorylated at Thr53 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 min).
The thiazide-sensitive sodium chloride cotransporter, NCC, is the major NaCl transport protein in the distal convoluted tubule (DCT) and plays an important role in maintaining blood pressure (Rosenbaek et al., 2014, Feng et al., 2015). Phosphorylation of NCC at Thr-53, Thr-58, and Ser-71 is an essential mediator of NCC function (Rosenbaek et al., 2014). NCC is constitutively cycled to the plasma membrane, and upon stimulation, it can be phosphorylated to both increase NCC activity and decrease NCC endocytosis, together increasing NaCl transport in the DCT (Feng et al., 2015).
Volume: 100 µL
Concentration: Lot Specific
Form: Antigen Affinity Purified from Pooled Serum
Host Species: Rabbit
Species Reactivity: Mouse
Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr53 conjugated to KLH
Protein Name / Synonyms: NCC, thiazide sensitive sodium chloride cotransporter, pNCC, phospho-NCC, NaCl cotransporter, p1311-53, SLC12A3, NCC T53, 53, p-NCC, NCC-t53, phosphoncc, phospho ncc, p131153
Gene ID: SLC12A3
Antibody Registry ID (RRID): AB_2650477
Physical State: Liquid
Buffer: 100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
Validation and Application Notes
Molecular Weight: 160
Aves Labs products are intended for use as research laboratory reagents. They are not intended for use as diagnostic or therapeutic reagents in humans.