We are the original manufacturer of our NCC (Thr53) rabbit polyclonal phosphospecific antibody, affinity purified from pooled serum. Optimized in WB and IHC.
Volume: 100 µL (Standard), 25 µL (Trial Size)
Concentration: Lot Specific
Clonality: Polyclonal
Form: Antigen Affinity Purified from Pooled Serum
Host Species: Rabbit
Species Reactivity: Mouse
Applications: WB
Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr53 conjugated to KLH
Protein Name / Synonyms: NCC, thiazide sensitive sodium chloride cotransporter, pNCC, phospho-NCC, NaCl cotransporter, p1311-53, SLC12A3, NCC T53, 53, p-NCC, NCC-t53, phosphoncc, phospho ncc, p131153
Target Description: The thiazide-sensitive sodium chloride cotransporter, NCC, is the major NaCl transport protein in the distal convoluted tubule (DCT) and plays an important role in maintaining blood pressure (Rosenbaek et al., 2014, Feng et al., 2015). Phosphorylation of NCC at Thr-53, Thr-58, and Ser-71 is an essential mediator of NCC function (Rosenbaek et al., 2014). NCC is constitutively cycled to the plasma membrane, and upon stimulation, it can be phosphorylated to both increase NCC activity and decrease NCC endocytosis, together increasing NaCl transport in the DCT (Feng et al., 2015).
Gene ID: SLC12A3
Antibody Registry ID (RRID): AB_2650477
Physical State: Liquid
Conjugate: Unconjugated
Buffer: 100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
Validation and Application Notes
Molecular Weight: 160
Storage
-20 C
NOTE
Aves Labs products are intended for use as research laboratory reagents. They are not intended for use as diagnostic or therapeutic reagents in humans.