Western blot of 10 µg of rat hippocampal lysate showing specific immunolabeling of the ~180 kDa NR2B subunit of the NMDA receptor.

Western blot of 10 µg of rat hippocampal lysate showing specific immunolabeling of the ~180 kDa NR2B subunit of the NMDA receptor.

from PhosphoSolutions

Anti-NMDA NR2B Subunit (100 ul) (1496-NR2B)

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Product Details


Excellent glutamatergic neuronal marker for use in IHC! We are the original manufacturer of our NMDAR NR2B rabbit polyclonal antibody, affinity purified from pooled serum. Optimized in WB, IHC, and IP.


Volume: 100 µL (Standard), 25 µL (Trial Size)

Concentration: Lot Specific

Clonality: Polyclonal

Form: Antigen Affinity Purified from Pooled Serum

Host Species: Rabbit

Species Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IP

Immunogen: Fusion protein from the C-terminal region of the NR2B subunit

Protein Name / Synonyms: GRIN, GRIN2B, GRIN2, GluN2b, nmdar2b, nmdanr2b

Target Description: The ion channels activated by glutamate that are sensitive to N-methyl-Daspartate (NMDA) are designated NMDA receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). The NMDA receptor is also one of the principal molecular targets for alcohol in the CNS (Lovinger et al., 1989; Alvestad et al., 2003; Snell et al., 1996). The rat NMDAR1 (NR1) was the first subunit of the NMDAR to be cloned and it can form NMDA activated channels when expressed in Xenopus oocytes but the currents in such channels are much smaller than those seen in situ. Channels with more physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits. Overexpression of the NR2B-subunit of the NMDA receptor has been associated with increases in learning and memory while aged, memory impaired animals have deficiencies in NR2B expression (Clayton et al., 2002a; Clayton et al., 2002b). The NMDAR is also potentiated by protein phosphorylation (Lu et al., 1999).

Gene ID: GRIN2B

Antibody Registry ID (RRID): AB_2492176

Physical State: Liquid

Conjugate: Unconjugated

Buffer: 100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Validation and Application Notes

Molecular Weight: 180

Storage

-20 C

NOTE

Aves Labs products are intended for use as research laboratory reagents. They are not intended for use as diagnostic or therapeutic reagents in humans.


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