Immunofluoresence of COS-7 cells expressing OLIG2-flag using AVES chicken anti-OLIG2 antibody (green) and rabbit anti-flag antibody (red). Blue is DAPI nuclear stain showing nuclei of both transfected and untransfected cells. Staining shows 100% correspondence between chicken anti-OLIG2 signal and anti-flag in transfected cells.
OLIG2 (also known as BHLHB1, BHLHE19, PRKCBP2, RACK17) is a basic helix-loop-helix (bHLH) transcription factor essential for oligodendrocyte and motoneuron development in the spinal cord (Ono et al., 2008). OLIG2 is also known to play a key role in mediating the brains response to cortical injury through post-injury gliosis (Chen Y et al., 2008) and is also highly expressed in malignant glioma where it has been implicated in glioma progression.
Affinity Purified IgY
ICC, IHC, WB
N-terminal half of human OLIG2 was used to immunize chickens to avoid cross-reactivity with OLIG3.
Human, Mouse, Rat
Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.
Eggs from hens hyperimmunized with target were used to prepare an IgY fraction which was then subjected to antigen-specific affinity purification.
Phosphate-buffered saline (10 mM) isotonic (0.9%, w/v) saline (“PBS," pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.
No cross reactivity reported.
Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used in Western blot on lysate from cells expressing Olig2.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.