Anti-Peripherin Antibody

Name: Anti-Peripherin Antibody
Brand: Aves Labs
SKU: PER
Price: 275.0 USD
Availability: InStock

from Aves Labs Datasheet

9 Citations

Full-length recombinant peripherin was injected into laying hens, and then the IgY fraction was purified from hyperimmunized animals. These antibodies have been validated with human, mouse and rat tissues.

Product Type

Primary Antibody

Species Reactivity

Human, Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Chicken

Format

IgY Fraction

Gene Name

PRPH NEF4 PRPH1

Molecular Weight

60 kDa

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Rat mixed neuron/glial cultures stained with anti-Peripherin (green) and rabbit anti α-internexin (red). Nuclei are stained with DAPI (blue).

Confocal micrographs of DRG cryosections from WT mouse double-labeled with antibodies against caldendrin (green) and NF200 (Cat no. NFH, 1:1000, purple) or peripherin (Cat no. PER, 1:1000, purple). Arrows and arrowheads indicate cells in which caldendrin is or is not, respectively, co-localized with NF200 or peripherin. Results are representative of at least 3 independent experiments. Scale bar, 100 µm. Image from publication CC-BY-4.0. PMID:36788334

Rat mixed neuron/glial cultures stained with anti-Peripherin (green) and rabbit anti α-internexin (red). Nuclei are stained with DAPI (blue).

Volume: 200 µL

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Price:

$275

Quantity:

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Product Details

Target

Perpherin

Target Description

Human Perpherin is a 53,651 dalton protein (470 amino acids) expressed in all PNS neurons and some CNS neurons, including a subset of cortical neurons, hippocampal neurons, and ventral horn spinal cord motor neurons. At an ultrastructural level, peripherin immunoreactivity is associated with the neurofilament portion of the cytoskeleton, being particularly abundant in the cell body. Peripherin protein has been shown to undergo upregulation during periods of trophic stress in cultured neurons, and mutations of the peripherin gene have been associated with the neurodegenerative disease amyotrophic lateral sclerosis.

Format

IgY Fraction

Concentration

2 mg/mL

Clonality

Polyclonal

Isotype

IgY

Applications

ICC, IHC, WB

Host Species

Chicken

Gene Name

PRPH NEF4 PRPH1

Molecular Weight

60 kDa

Antigen

Recombinant rat peripherin expressed in Escherichia coli

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2313558

Storage

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.

Physical State

Liquid

Production Notes

Chickens were immunized with full-length recombinant peripherin protein. After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. Antibodies were diluted in the buffer described above, and then filter-sterilized.

Buffer

Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline ("PBS" pH 7.2) with bovine serum albumin (BSA, 0.5%) added to prevent absorption to the plastic and sodium azide (0.02%, w/v) added as a preservative

Dilution Ranges

WB: 1:2000-1:5000
IHC: 1:2000-1:5000
ICC: 1:2000-1:5000

Quality Control

Quality assurance analysis was performed using immunohistochemistry (at a dilution of 1:10000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

12 months from receipt of product

Shipping

Ice packs

Synonyms

Peripherin (Neurofilament 4)

UniProt Details

UniProt (Immunogen Species): P41219

Product Specific References for Applications and Species

Immunocytochemistry: Human | Rat
Immunohistochemistry: Mouse | Rat
Additional Publications: Unspecified

Immunocytochemistry: Human
PMID	Dilution	Publication
34930944	not listed	Alsaloum, M., et al. 2021. Contributions of NaV1.8 and NaV1.9 to excitability in human induced pluripotent stem-cell derived somatosensory neurons. Scientific Reports, 24283.
34557669	1:200	Yuan, J.H., et al. 2021. KCNQ variants and pain modulation: a missense variant in Kv7.3 contributes to pain resilience. Brain Communications, fcab212.
23867767	1:100-1:250	Rose, K.E., et al. 2013. Immunohistological demonstration of CaV3. 2 T-type voltage-gated calcium channel expression in soma of dorsal root ganglion neurons and peripheral axons of rat …. Neuroscience, 263-274.

Immunocytochemistry: Rat
PMID	Dilution	Publication
31681845	1:500	Akin, E.J., et al. 2019. Building sensory axons: Delivery and distribution of NaV1.7 channels and effects of inflammatory mediators. Science Advances, eaax4755.

Immunohistochemistry: Mouse
PMID	Dilution	Publication
29022879	not listed	Olson, W., et al. 2017. Sparse genetic tracing reveals regionally specific functional organization of mammalian nociceptors. eLife, e29507.
28276837	1:1000	Chamessian, A.G., et al. 2017. 5-Hydroxymethylcytosine (5hmC) and Ten-eleven translocation 1–3 (TET1–3) proteins in the dorsal root ganglia of mouse: Expression and dynamic regulation in neuropathic pain. Somatosensory & Motor Research, 72-79.

Immunohistochemistry: Rat
PMID	Dilution	Publication
27385723	1:1000	Ramachandra, R., et al. 2016. Voltage-dependent sodium (NaV) channels in group IV sensory afferents. Molecular Pain, .
24966300	1:1000	Baxter, J.C., et al. 2014. Functional expression of α7-nicotinic acetylcholine receptors by muscle afferent neurons. Journal of Neurophysiology, 1549-1558.

Additional Publications: Unspecified
PMID	Publication
22173870	Simon, C., et al. 2012. Sox10‐iCreERT2: A mouse line to inducibly trace the neural crest and oligodendrocyte lineage. Genesis, 506-515.

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Anti-Peripherin Antibody

Full-length recombinant peripherin was injected into laying hens, and then the IgY fraction was purified from hyperimmunized animals. These antibodies have been validated with human, mouse and rat tissues.