Anti-Prion Protein Antibody

Name: Anti-Prion Protein Antibody
Brand: Aves Labs
SKU: PRN
Price: 275.0 USD
Availability: InStock

from Aves Labs Datasheet

Antibodies were prepared by injecting laying hens with purified recombinant human PRN protein. After a series of boosts, eggs were collected from hyperimmunized aniamsl and the IgY fraction prepared. Affinity purified antibodies were then isolated from the IgY fraction using a PRN protein column. Finally, affinity purified antibodies were mixed with the IgY fraction to make the final product. These antibodies have been validated with human, mouse and rat tissues.

Product Type

Primary Antibody

Species Reactivity

Human, Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Chicken

Format

Mixture of IgY fraction and affinity-purified antibodies

Gene Name

PRNP ALTPRP PRIP PRP

Molecular Weight

27 kDa

Go to Full Product Details

Mouse fibroblast cultures transfected with a recombinant fragment of the mouse prion protein (green staining). Non-infected cells show labeled nuclei (blue staining) due to treatment with Hoechst stain.

Volume: 200 µL

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$275

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Bulk Order Anti-Prion Protein Antibody

Product Details

Target

Prion Protein (PRN)

Target Description

Prion protein is a ubiquitously-expressed, 27661 dalton protein (253 amino acids, NP_898902.1) of unclear function(s) in eukaryotes. This protein can exist in one of two conformational forms. One is its normally-folded form (PrPc or "cellular PrP"), which contains a mixture of alpha helical regions and a few beta-pleated sheets. The other is a misfolded form (PrPsc or "scrapie PrP") whose structure contains mostly beta-pleated sheets. This misfolded scrapie PrP form is cytotoxic, especially to nervous tissue, and can serve as a "seed," inducing adjacent, normally-folded PrP proteins to misfold into the cytotoxic conformation. In this sense, the scrapie PrP can be thought of as an infectious agent both within the organism as well between organisms.

Format

Mixture of IgY fraction and affinity-purified antibodies

Concentration

10 mg/mL

Clonality

Polyclonal

Isotype

IgY

Applications

ICC, IHC, WB

Host Species

Chicken

Gene Name

PRNP ALTPRP PRIP PRP

Molecular Weight

27 kDa

Antigen

Reccombinant fragment of human prion

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2313559

Storage

Store at -20°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile.

Physical State

Liquid

Production Notes

Chickens were immunized with a recombinant fragment of the human prion protein emulsified with Freund’s complete and incomplete adjuvants.After multiple injections, eggs were collected from the hens, and IgY fractions were prepared from the yolks.Affinity-purified antibodies were then prepared using an agarose column to which the recombinant protein fragments were covalently attached. Finally, affinity purified antibodies (50 µg/mL final concentration) were mixed with the IgY fraction (10 mg/mL final concentration) in a mixture containing glycerol (50% v/v) (to prevent freezing at -20°C) and 0.02% sodium azide.

Buffer

Sodium phosphate (10 mM, pH 7.2) buffered isotonic saline (0.9%, w/v), glycerol (50%, v/v), with sodium azide (0.02%,w/v) as an anti-microbial agent.

Dilution Ranges

WB: 1:2500-1:5000
IHC: 1:1000-1:2000
ICC: 1:1000-1:2000

Quality Control

Antibodies were analyzed by immunocytochemistry (1:1000 dilution) using cells transfected with the prion protein cDNA.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

12 months from receipt of product

Shipping

Ice packs

Synonyms

Major prion protein (PrP) (ASCR) (PrP27-30) (PrP33-35C) (CD antigen CD230)

UniProt Details

UniProt (Immunogen Species): P04156

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Anti-Prion Protein Antibody