Immunostaining of retinal ganglion cells from the blind mole rat, Spalax Ehrenbergi, stained for RBPMS (red), melanopsin (green), and DAPI (blue). Credit: Jens Hannibal, Ass. Professor, MD, PhD., DmSc. Dept. Clinical Biochemistry, Bispebjerg Hospital, University of Copenhagen, Denmark.
Immunostaining of rat retinal ganglion cells showing specific immunolabeling of RBPMS(1:500) in green. Photo courtesy of Brian Choi, Univ. of Toronto.
Immunolabeling of macaque retinal ganglion cells specific immunolabelling of RBPMS (green, 1:500). The pink stain is Hoechst. The frozen sections were harvested and then fixed with 4% PFA for 30 minutes (A,B) or perfused and then fixed with 4% PFA (C,D). Retinas samples were blocked with 10% NHS for 1 hour and incubated with primary antibody overnight with 3% NHS. These images were kindly provided by Jacqueline Gayet from UC- Berkley School of Optometry.
RBPMS (RNA binding protein with multiple splicing), also known as HERMES, contains one RRM (RNA recognition motif) domain and belongs to the RRM family of RNA-binding proteins. RBPMS exists as multiple alternatively spliced isoforms and is thought to bind RNA, possibly playing a role in RNA-related events, such as transcription and translation. RNA-binding proteins that are specific to retinal ganglion cells (RGCs) have been previously identified as excellent markers for RGCs (Kwong et al., 2010). Recent findings show that antibodies against RBPMS are robust reagents that exclusively identify RGCs in multiple mammalian species (Rodriguez et al. 2014).
Volume: 100 µL
Concentration: Lot Specific
Form: Antigen Affinity Purified
Host Species: Rabbit
Applications: WB, IHC
Immunogen: Synthetic peptide corresponding to amino acid residues from the N-terminal region conjugated to KLH
Gene ID: RBPMS
Antibody Registry ID (RRID): AB_2492226
Physical State: Liquid
Buffer: PBS + 0.03% NaN3
Validation and Application Notes
Molecular Weight: 24
Aves Labs products are intended for use as research laboratory reagents. They are not intended for use as diagnostic or therapeutic reagents in humans.