Immunostaining of retinal ganglion cells from the blind mole rat, Spalax Ehrenbergi, stained for RBPMS (red), melanopsin (green), and DAPI (blue). Credit: Jens Hannibal, Ass. Professor, MD, PhD., DmSc. Dept. Clinical Biochemistry, Bispebjerg Hospital, University of Copenhagen, Denmark.

Immunostaining of retinal ganglion cells from the blind mole rat, Spalax Ehrenbergi, stained for RBPMS (red), melanopsin (green), and DAPI (blue). Credit: Jens Hannibal, Ass. Professor, MD, PhD., DmSc. Dept. Clinical Biochemistry, Bispebjerg Hospital, University of Copenhagen, Denmark.

Immunostaining of rat retinal ganglion cells showing specific immunolabeling of RBPMS(1:500) in green. Photo courtesy of Brian Choi, Univ. of Toronto.

Immunostaining of rat retinal ganglion cells showing specific immunolabeling of RBPMS(1:500) in green. Photo courtesy of Brian Choi, Univ. of Toronto.

Western blot of rat heart lysate showing specific labeling of the ~24 kDa RBPMS protein.

Western blot of rat heart lysate showing specific labeling of the ~24 kDa RBPMS protein.

Immunolabeling of macaque retinal ganglion cells specific immunolabelling of RBPMS (green, 1:500). The pink stain is Hoechst. The frozen sections were harvested and then fixed with 4% PFA for 30 minutes (A,B) or perfused and then fixed with 4% PFA (C,D). Retinas samples were blocked with 10% NHS for 1 hour and incubated with primary antibody overnight with 3% NHS. These images were kindly provided by Jacqueline Gayet from UC- Berkley School of Optometry.

Immunolabeling of macaque retinal ganglion cells specific immunolabelling of RBPMS (green, 1:500). The pink stain is Hoechst. The frozen sections were harvested and then fixed with 4% PFA for 30 minutes (A,B) or perfused and then fixed with 4% PFA (C,D). Retinas samples were blocked with 10% NHS for 1 hour and incubated with primary antibody overnight with 3% NHS. These images were kindly provided by Jacqueline Gayet from UC- Berkley School of Optometry.

Anti-RBPMS

Anti-RBPMS

Immunostaining of mouse flat mounted retina showing specific immunolabeling of Anti-RBPMS (catalog #1832-RBPMS, 1:250). Photo courtesy of the Feldhem Lab at University of California, Santa Cruz.

Immunostaining of mouse flat mounted retina showing specific immunolabeling of Anti-RBPMS (catalog #1832-RBPMS, 1:250). Photo courtesy of the Feldhem Lab at University of California, Santa Cruz.

from PhosphoSolutions

Anti-RBPMS (1832-RBPMS)

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Product Details


Details

Volume: 100 µL (Standard), 25 µL (Trial Size)

Concentration: Lot Specific

Clonality: Polyclonal

Form: Antigen Affinity Purified

Host Species: Rabbit

Species Reactivity: Guinea Pig, Human, Monkey, Mouse, Rabbit, Rat, Tree Shrew, Blind Mole Rat

Applications: WB, IHC

Immunogen: Synthetic peptide corresponding to amino acid residues from the N-terminal region conjugated to KLH

Target Description: RBPMS (RNA binding protein with multiple splicing), also known as HERMES, contains one RRM (RNA recognition motif) domain and belongs to the RRM family of RNA-binding proteins. RBPMS exists as multiple alternatively spliced isoforms and is thought to bind RNA, possibly playing a role in RNA-related events, such as transcription and translation. RNA-binding proteins that are specific to retinal ganglion cells (RGCs) have been previously identified as excellent markers for RGCs (Kwong et al., 2010). Recent findings show that antibodies against RBPMS are robust reagents that exclusively identify RGCs in multiple mammalian species (Rodriguez et al. 2014).

Gene ID: RBPMS

Antibody Registry ID (RRID): AB_2492226

Physical State: Liquid

Conjugate: Unconjugated

Buffer: PBS + 0.03% NaN3

Validation and Application Notes

Molecular Weight: 24

Storage

-20 C

NOTE

Aves Labs products are intended for use as research laboratory reagents. They are not intended for use as diagnostic or therapeutic reagents in humans.


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