Anti-RBPMS Antibody
Our Anti-RBPMS primary antibody from PhosphoSolutions is rabbit polyclonal. It detects guinea pig, human, mouse, non-human primate, rabbit, and rat RBPMS and is antigen affinity purified. It is great for use in WB, IHC, ICC.
Polyclonal
ICC, IHC, WB
Guinea Pig
RBPMS
24 kDa
Western blot of rat heart lysate showing specific labeling of the ~24 kDa RBPMS protein.
Anti-RBPMS Antibody
SKU: 1832-RBPMS
Product Details
RBPMS
RBPMS (RNA binding protein with multiple splicing), also known as HERMES, contains one RRM (RNA recognition motif) domain and belongs to the RRM family of RNA-binding proteins. RBPMS exists as multiple alternatively spliced isoforms and is thought to bind RNA, possibly playing a role in RNA-related events, such as transcription and translation. RNA-binding proteins that are specific to retinal ganglion cells (RGCs) have been previously identified as excellent markers for RGCs (Kwong et al., 2010). Recent findings show that antibodies against RBPMS are robust reagents that exclusively identify RGCs in multiple mammalian species (Rodriguez et al. 2014).
Antigen Affinity Purified
Polyclonal
IgG
ICC, IHC, WB
Guinea Pig
RBPMS
24 kDa
Synthetic peptide corresponding to amino acid residues from the N-terminal region of rat RBPMS, conjugated to keyhole limpet hemocyanin (KLH).
Blind Mole, Guinea Pig, Human, Monkey, Mouse, Rabbit, Rat, Tree Shrew
AB_2492226
Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.
Liquid
The antibody is prepared from guinea pig serum by affinity purification via chromatography on an affinity column prepared with the N-terminal peptide used as antigen.
PBS + 0.03% NaN3
WB: 1:1000
IHC: 1:50-1:20,000
ICC: 1:1000
IP: Not tested - want to try?
IHC: 1:50-1:20,000
ICC: 1:1000
IP: Not tested - want to try?
Unconjugated
Specific for endogenous levels of the ~24 kDa RBPMS protein.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Blue Ice
FLJ32971 antibody, Heart and RRM expressed sequence antibody, Hermes antibody, RBP MS antibody, RBP-MS antibody, Rbpms antibody, RBPMS_HUMAN antibody, RNA binding protein gene with multiple splicing antibody, RNA-binding protein with multiple splicing antibody
UniProt (Human): Q93062
UniProt (Immunogen Species): Q9WVB0
UniProt (Immunogen Species): Q9WVB0
19663
Citations (62)
Product Specific References for Applications and Species
- Immunocytochemistry: Human | Mouse
- Immunohistochemistry: Human | Mouse | Primate | Rabbit | Rat | Tree Shrew
- Western Blot: Human | Mouse
| Immunocytochemistry: Human | ||
| PMID | Dilution | Publication |
| 31155355 | not listed | Chang, K.C., et al. 2019. Opposing Effects of Growth and Differentiation Factors in Cell-Fate Specification. Current Biology, 29(12), pp. 1963-1975. |
| Immunocytochemistry: Mouse | ||
| PMID | Dilution | Publication |
| 32164319 | not listed | Yang, N., et al. 2020. The Susceptibility of Retinal Ganglion Cells to Optic Nerve Injury is Type Specific. Cells, 9(3), p.677. |
| 31155355 | not listed | Chang, K.C., et al. 2019. Opposing Effects of Growth and Differentiation Factors in Cell-Fate Specification. Current Biology, 29(12), pp. 1963-1975. |
| 30018341 | not listed | Rheaume, B.A., et al. 2018. Single cell transcriptome profiling of retinal ganglion cells identifies cellular subtypes. Nature Communications, 9(1), p.2759. |
| 29576390 | 1:1000 | Sarin, S., et al. 2018. Role for Wnt signaling in retinal neuropil development: analysis via RNA-seq and in vivo somatic CRISPR mutagenesis. Neuron, 98(1), pp.109-126. |
| Immunohistochemistry: Human | ||
| PMID | Dilution | Publication |
| 33009001 | 1:500 | Masri, R.A., et al. 2020. Analysis of Parvocellular and Magnocellular Visual Pathways in Human Retina. Journal of Neuroscience, 40(42), pp.8132-8148. |
| 31883839 | 1:500 | Werneburg, S., et al. 2020. Targeted complement inhibition at synapses prevents microglial synaptic engulfment and synapse loss in demyelinating disease. Immunity, 52(1), pp.167-182. |
| 29665009 | 1:500 | Christiansen, A.T., et al. 2018. Localization, distribution, and connectivity of neuropeptide Y in the human and porcine retinas—A comparative study. Journal of Comparative Neurology, 526(12), pp. 1877-1895. |
| 28399269 | not listed | Obara, E.A., et al. 2017. Loss of Melanopsin-Expressing Retinal Ganglion Cells in Patients With Diabetic Retinopathy. Investigative Ophthalmology and Visual Science, 58(4), pp.2187-2192. |
| 27583827 | 1:500 | Obara, E. A., et al. 2016. Loss of Melanopsin-Expressing Retinal Ganglion Cells in Severely Staged Glaucoma Patients. Investigative Ophthalmology and Visual Science, 57(11), pp. 4661-4667. |
| Immunohistochemistry: Mouse | ||
| PMID | Dilution | Publication |
| 33784498 | 1:1000 | Johnson, K.P., et al. 2021. Cell-type-specific binocular vision guides predation in mice. Neuron. |
| 33789891 | 1:500 | Khatib, T.Z., et al. 2021. Receptor-ligand supplementation via a self-cleaving 2A peptide–based gene therapy promotes CNS axonal transport with functional recovery. Science Advances, 7(14), p.eabd2590. |
| 33657370 | 1:500 | Lindborg, J.A., et al. 2021. Optic nerve regeneration screen identifies multiple genes restricting adult neural repair. Cell Reports, 34(9), p.108777. |
| 33434618 | 1:500 | Shabanzadeh, A.P., et al. 2021. Cholesterol synthesis inhibition promotes axonal regeneration in the injured central nervous system. Neurobiology of Disease, p.105259. |
| 33397711 | 1:1000 | Pottackal, J., et al. 2021. Photoreceptive Ganglion Cells Drive Circuits for Local Inhibition in the Mouse Retina. Journal of Neuroscience, Jan 4, JN-RM-0674-20. |
| 33293617 | 1:500 | Groleau, M., et al. 2020. Mesoscopic cortical network reorganization during recovery of optic nerve injury in GCaMP6s mice. Scientific Reports, 10(1), pp.1-13. |
| 33207201 | 1:500 | Kerstein, P.C., et al. 2020. Gbx2 identifies two amacrine cell subtypes with distinct molecular, morphological, and physiological properties. Cell Reports, 33(7), p.108382. |
| 33101779 | 1:200 | Yu, H., et al. 2020. Mitochondrial transfer of the mutant human ND6T14484C gene causes visual loss and optic neuropathy. Translational Vision Science & Technology, 9(11), pp.1-1. |
| 33045220 | 1:1000 | Yabana, T., et al. 2020. GCaMP3 expressing cells in the ganglion cell layer of Thy1-GCaMP3 transgenic mice before and after optic nerve injury. Experimental Eye Research, p.108297. |
| 33039455 | 1:300 | Tao, X., et al. 2020. Single transient intraocular pressure elevations cause prolonged retinal ganglion cell dysfunction and retinal capillary abnormalities in mice. Experimental Eye Research, p.108296. |
| 32910942 | 1:500 | Flood, M.D., et al. 2020. Early diabetes impairs ON sustained ganglion cell light responses and adaptation without cell death or dopamine insensitivity. Experimental Eye Research, 200, p.108223. |
| 32801314 | 1:500 | de Araújo, D.S.M., et al. 2020. TRPA1 mediates damage of the retina induced by ischemia and reperfusion in mice. Cell Death & Disease, 11(8), pp.1-14. |
| 32798466 | 1:500 | Romano, G.L., et al. 2020. P2X7 receptor antagonism preserves retinal ganglion cells in glaucomatous mice. Biochemical Pharmacology, 180, p.114199. |
| 32605122 | 1:500 | Chou, T.H., et al. 2020. Nicotinamide-Rich Diet in DBA/2J Mice Preserves Retinal Ganglion Cell Metabolic Function as Assessed by PERG Adaptation to Flicker. Nutrients, 12(7), p.1910. |
| 32494665 | not listed | Prosseda, P.P., et al. 2020. Optogenetic stimulation of phosphoinositides reveals a critical role of primary cilia in eye pressure regulation. Science Advances, 6(18), p.eaay8699. |
| 32457074 | 1:1000 | Yan, W., et al. 2020. Mouse Retinal Cell Atlas: Molecular Identification of Over Sixty Amacrine Cell Types. Journal of Neuroscience, JN-RM-0471-20. |
| 32029703 | 1:200 | Mdzomba, J.B., et al. 2020. Nogo-A-targeting antibody promotes visual recovery and inhibits neuroinflammation after retinal injury. Cell Death and Disease, 11(2), pp.1-16. |
| 31784286 | not listed | Tran, N.M., et al. 2019. Single-Cell Profiles of Retinal Ganglion Cells Differing in Resilience to Injury Reveal Neuroprotective Genes. Neuron, 104(6), pp.1039-1055. |
| 31775817 | 1:1000 | Welsbie, D.S., et al. 2019. Targeted disruption of dual leucine zipper kinase and leucine zipper kinase promotes neuronal survival in a model of diffuse traumatic brain injury. Molecular Neurodegeneration, 14(1), p.44. |
| 31722722 | 1:1000 | Alrashdi, B., et al. 2019. Nav1. 6 promotes inflammation and neuronal degeneration in a mouse model of multiple sclerosis. Journal of Neuroinflammation, 16(1), pp.1-13. |
| 31609468 | not listed | Jiang, D., et al. 2019. Spatiotemporal gene expression patterns reveal molecular relatedness between retinal laminae. Journal of Comparative Neurology, 528(5), pp. 729-755. |
| 30640971 | 1:1000 | Blandford, S.N., et al. 2019. Retinal Characterization of the Thy1-GCaMP3 Transgenic Mouse Line After Optic Nerve Transection. Investigative Ophthalmology and Visual Science, 60(1), pp.183-191. |
| 27391320 | 1:500 | Ghinia, M.G., et al. 2019. Brn3a and Brn3b knockout mice display unvaried retinal fine structure despite major morphological and numerical alterations of ganglion cells. Journal of Comparative Neurology, 527(1), pp. 187-211. |
| 30487225 | 1:300 | Milosavljevic, N., et al. 2018. Photoreceptive retinal ganglion cells control the information rate of the optic nerve. Proceedings of the National Academy of Sciences, 115(50), pp.E11817-E11826. |
| 30312782 | 1:2000 | Yee, C.W., et al. 2018. Atypical expression and activation of GluN2A and GluN2B-containing NMDA receptors at ganglion cells during retinal degeneration. Neuroscience, 393, pp.61-72. |
| 30197236 | 1:1000 | Duan, X., et al. 2018. Cadherin Combinations Recruit Dendrites of Distinct Retinal Neurons to a Shared Interneuronal Scaffold. Neuron, 99(6), pp.1145-1154. |
| 30076594 | 1:500 | Lilley, B.N., et al. 2018. Genetic access to neurons in the accessory optic system reveals a role for Sema6A in midbrain circuitry mediating motion perception. Journal of Comparative Neurology, 527(1), pp.282-296. |
| 30018341 | not listed | Rheaume, B.A., et al. 2018. Single cell transcriptome profiling of retinal ganglion cells identifies cellular subtypes. Nature communications, 9(1), p.2759. |
| 29937281 | 1:200 | Drinnenberg, A., et al. 2018. How Diverse Retinal Functions Arise from Feedback at the First Visual Synapse. Neuron, 99(1), pp. 117-134. |
| 29931057 | 1:1000 | Agostinone, J., et al. 2018. Insulin signalling promotes dendrite and synapse regeneration and restores circuit function after axonal injury. Brain, 141(7), pp. 1963-1980. |
| 29576390 | 1:50 | Sarin, S., et al. 2018. Role for Wnt signaling in retinal neuropil development: analysis via RNA-seq and in vivo somatic CRISPR mutagenesis. Neuron, 98(1), pp.109-126. |
| 29632360 | 1:500 | Liu, J., et al. 2018. Tbr1 instructs laminar patterning of retinal ganglion cell dendrites. Nature Neuroscience, 21(5), pp. 659-670. |
| 29439167 | 1:800 | Ing-Esteves, S., et al. 2018. Combinatorial Effects of Alpha-and Gamma-Protocadherins on Neuronal Survival and Dendritic Self-Avoidance. Journal of Neuroscience, 38(11), pp.2713-2729. |
| 28760865 | 1:500 | Clements, R., et al. 2017. Dystroglycan maintains inner limiting membrane integrity to coordinate retinal development. Journal of Neuroscience, 37(35), pp. 8559-8574. |
| 29241535 | 1:2000 | Seabrook, T.A., et al. 2017. Strict Independence of Parallel and Poly-synaptic Axon-Target Matching during Visual Reflex Circuit Assembly. Cell Reports, 21(11), pp.3049-3064. |
| 29106385 | not listed | Livne-Bar, I., et al. 2017. Astrocyte-derived lipoxins A 4 and B 4 promote neuroprotection from acute and chronic injury. The Journal of Clinical Investigation, 127(12), pp.4403-4414. |
| 28866734 | 1:4000 | Ruzafa, N., et al. 2017. The Retina of Osteopontin deficient Mice in Aging. Molecular Neurobiology, 55(1), pp.213-221. |
| 28781169 | 1:5000 | Peng, Y.R., et al. 2017. Satb1 Regulates Contactin 5 to Pattern Dendrites of a Mammalian Retinal Ganglion Cell. Neuron, 95(4), pp. 869-883. |
| 28607486 | not listed | Sabbah, S., et al. 2017. A retinal code for motion along the gravitational and body axes. Nature, 546(7659), pp.492-497. |
| 28472856 | 1:1000 | Pérez de Sevilla Müller, L., et al. 2017. Multiple cell types form the VIP amacrine cell population. Journal of Comparative Neurology, 527(1), pp.133-158. |
| 27699209 | not listed | Ueki, Y., et al. 2016. Loss of Ikbkap causes slow, progressive retinal degeneration in a mouse model of Familial Dysautonomia. Eneuro, 3(5), p. ENEURO-0143. |
| 27001178 | not listed | Boggio, E. M., et al. 2016. Visual impairment in FOXG1-mutated individuals and mice. Neuroscience, 324,pp. 496-508. |
| 25631988 | 1:20,000 | Pérez de Sevilla Müller, L., et al. 2015. Expression and cellular localization of the voltage-gated calcium channel α2δ3 in the rodent retina. Journal of Comparative Neurology, 523(10), 1443-1460. |
| Immunohistochemistry: Primate | ||
| PMID | Dilution | Publication |
| 33527361 | 1:500 | Nasir-Ahmad, S., et al. 2021. Identification of retinal ganglion cell types expressing the transcription factor Satb2 in three primate species. Journal of Comparative Neurology. |
| 30377226 | 1:1000 | Dhande, O.S., et al. 2019. Molecular Fingerprinting of On–Off Direction-Selective Retinal Ganglion Cells Across Species and Relevance to Primate Visual Circuits. Journal of Neuroscience, 39(1), pp. 78-95. |
| 27568514 | 1:500 | Long, Y., et al. 2016. Wide-field diffuse amacrine cells in the monkey retina contain immunoreactive Cocaine-and Amphetamine-Regulated Transcript (CART). Peptides, 84, pp. 22-35. |
| Immunohistochemistry: Rabbit | ||
| PMID | Dilution | Publication |
| 30377226 | 1:1000 | Dhande, O.S., et al. 2019. Molecular Fingerprinting of On–Off Direction-Selective Retinal Ganglion Cells Across Species and Relevance to Primate Visual Circuits. Journal of Neuroscience, 39(1), pp. 78-95. |
| Immunohistochemistry: Rat | ||
| PMID | Dilution | Publication |
| 33789891 | 1:500 | Khatib, T.Z., et al. 2021. Receptor-ligand supplementation via a self-cleaving 2A peptide–based gene therapy promotes CNS axonal transport with functional recovery. Science Advances, 7(14), p.eabd2590. |
| 31369591 | 1:500 | Choi, B.K., et al. 2019. Stabilization of primary cilia reduces abortive cell cycle re-entry to protect injured adult CNS neurons from apoptosis. PloS One, 14(8), p.e0220056. |
| 31337818 | 1:1000 | Kreymerman, A., et al. 2019. MTP18 is a Novel Regulator of Mitochondrial Fission in CNS Neuron Development, Axonal Growth, and Injury Responses. Scientific Reports, 9(1), p.10669. |
| 27375437 | 1:500 | Esquiva, G., et al. 2016. Non-image forming light detection by melanopsin, rhodopsin, and long-middlewave (L/W) cone opsin in the subterranean blind mole rat, Spalax ehrenbergi: immunohistochemical characterization, distribution, and connectivity. Frontiers in Neuroanatomy, 10, p.61. |
| Immunohistochemistry: Tree Shrew | ||
| PMID | Dilution | Publication |
| 29238991 | 1:500 | Johnson, E.N., et al. 2017. Distribution and diversity of intrinsically photosensitive retinal ganglion cells in tree shrew. Journal of Comparative Neurology, 527(1), pp.328-344. |