Anti-RBPMS Antibody

Name: Anti-RBPMS Antibody
Brand: PhosphoSolutions
SKU: 1832-RBPMS
Price: 399.0 USD
Availability: InStock

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Our Anti-RBPMS primary antibody from PhosphoSolutions is guinea pig polyclonal. It detects guinea pig, human, mouse, non-human primate, rabbit, and rat RBPMS and is antigen affinity purified. It is great for use in WB, IHC, ICC.

Product Type

Primary Antibody

Species Reactivity

Blind Mole, Guinea Pig, Human, Monkey, Mouse, Rabbit, Rat, Tree Shrew

Expected Reactivity

Canine, Hamster, Human

Applications

ICC, IHC, WB

Host Species

Guinea Pig

Format

Antigen Affinity Purified

Gene Name

RBPMS

Molecular Weight

24 kDa

Go to Full Product Details

Immunostaining of retinal ganglion cells from the blind mole rat, Spalax Ehrenbergi, stained for RBPMS (red), melanopsin (green), and DAPI (blue). Credit: Jens Hannibal, Ass. Professor, MD, PhD., DmSc. Dept. Clinical Biochemistry, Bispebjerg Hospital, University of Copenhagen, Denmark.

Immunostaining of mouse flat mounted retina showing specific immunolabeling of Anti-RBPMS (cat. 1832-RBPMS, 1:250). Photo courtesy of the Feldhem Lab at University of California, Santa Cruz.

Immunostaining of rat retinal ganglion cells showing specific immunolabeling of RBPMS(1:500) in green. Photo courtesy of Brian Choi, Univ. of Toronto.

Purified mouse RGCs in culture that have been probed by FISH for Runx1(red), immunostained with an RGC marker RBPMS(green, cat. 1832-RBPMS), and DAPI(blue). (A) An section where two Runx1+ RGCs outlined with dashed box are shown enlarged below (B,C; cell soma outlined with dashed line). Photo courtesy Bruce Rheaume, University of Connecticut. CC-BY-4.0

Western blot of rat heart lysate showing specific labeling of the ~24 kDa RBPMS protein.

Volume: 100 µL

Variant

Price:

$399

Quantity:

Bulk Order Anti-RBPMS Antibody

Product Details

Target

RBPMS

Target Description

RBPMS (RNA binding protein with multiple splicing), also known as HERMES, contains one RRM (RNA recognition motif) domain and belongs to the RRM family of RNA-binding proteins. RBPMS exists as multiple alternatively spliced isoforms and is thought to bind RNA, possibly playing a role in RNA-related events, such as transcription and translation. RNA-binding proteins that are specific to retinal ganglion cells (RGCs) have been previously identified as excellent markers for RGCs (Kwong et al., 2010). Recent findings show that antibodies against RBPMS are robust reagents that exclusively identify RGCs in multiple mammalian species (Rodriguez et al. 2014).

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

ICC, IHC, WB

Host Species

Guinea Pig

Gene Name

RBPMS

Molecular Weight

24 kDa

Antigen

Synthetic peptide corresponding to amino acid residues from the N-terminal region of rat RBPMS, conjugated to keyhole limpet hemocyanin (KLH).

Species Reactivity

Blind Mole, Guinea Pig, Human, Monkey, Mouse, Rabbit, Rat, Tree Shrew

Expected Reactivity

Canine, Hamster, Human

Antibody Registry ID

AB_2492226

Storage

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Physical State

Liquid

Production Notes

The antibody is prepared from guinea pig serum by affinity purification via chromatography on an affinity column prepared with the N-terminal peptide used as antigen.

Buffer

PBS + 0.03% NaN3

Dilution Ranges

WB: 1:1000
IHC: 1:50-1:20,000
ICC: 1:1000
IP: Not tested - want to try?

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~24 kDa RBPMS protein.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

FLJ32971 antibody, Heart and RRM expressed sequence antibody, Hermes antibody, RBP MS antibody, RBP-MS antibody, Rbpms antibody, RBPMS_HUMAN antibody, RNA binding protein gene with multiple splicing antibody, RNA-binding protein with multiple splicing antibody

UniProt Details

UniProt (Human): Q93062
UniProt (Immunogen Species): Q9WVB0

NCBI Gene ID

19663

Product Specific References for Applications and Species

Immunocytochemistry: Human | Mouse
Immunohistochemistry: Human | Mouse | Primate | IHCRabbit | Rat | Tree Shrew
Western Blot: Human | Mouse

Immunocytochemistry: Human
PMID	Dilution	Publication
31155355	not listed	Chang, K.C., et al. 2019. Opposing Effects of Growth and Differentiation Factors in Cell-Fate Specification. Current Biology, 29(12), pp. 1963-1975.

Immunocytochemistry: Mouse
PMID	Dilution	Publication
32164319	not listed	Yang, N., et al. 2020. The Susceptibility of Retinal Ganglion Cells to Optic Nerve Injury is Type Specific. Cells, 9(3), p.677.
31155355	not listed	Chang, K.C., et al. 2019. Opposing Effects of Growth and Differentiation Factors in Cell-Fate Specification. Current Biology, 29(12), pp. 1963-1975.
30018341	not listed	Rheaume, B.A., et al. 2018. Single cell transcriptome profiling of retinal ganglion cells identifies cellular subtypes. Nature Communications, 9(1), p.2759.
29576390	1:1000	Sarin, S., et al. 2018. Role for Wnt signaling in retinal neuropil development: analysis via RNA-seq and in vivo somatic CRISPR mutagenesis. Neuron, 98(1), pp.109-126.

Immunohistochemistry: Human
PMID	Dilution	Publication
35029299	1:500	Baldicano, A.K., et al. 2022. Retinal Ganglion Cells Expressing CaM Kinase II in Human and Nonhuman Primates. The Journal of Comparative Neurology.
34187514	0.25ug/ml	Belforte, N., et al. 2021. AMPK hyperactivation promotes dendrite retraction, synaptic loss, and neuronal dysfunction in glaucoma. Molecular neurodegeneration, 16(1), pp.1-19.
33009001	1:500	Masri, R.A., et al. 2020. Analysis of Parvocellular and Magnocellular Visual Pathways in Human Retina. Journal of Neuroscience, 40(42), pp.8132-8148.
31883839	1:500	Werneburg, S., et al. 2020. Targeted complement inhibition at synapses prevents microglial synaptic engulfment and synapse loss in demyelinating disease. Immunity, 52(1), pp.167-182.
29665009	1:500	Christiansen, A.T., et al. 2018. Localization, distribution, and connectivity of neuropeptide Y in the human and porcine retinas—A comparative study. Journal of Comparative Neurology, 526(12), pp. 1877-1895.
28399269	not listed	Obara, E.A., et al. 2017. Loss of Melanopsin-Expressing Retinal Ganglion Cells in Patients With Diabetic Retinopathy. Investigative Ophthalmology and Visual Science, 58(4), pp.2187-2192.
27583827	1:500	Obara, E. A., et al. 2016. Loss of Melanopsin-Expressing Retinal Ganglion Cells in Severely Staged Glaucoma Patients. Investigative Ophthalmology and Visual Science, 57(11), pp. 4661-4667.

Immunohistochemistry: Mouse
PMID	Dilution	Publication
35524141	1:1000	Tsai, N.Y., et al. 2022. Trans-Seq maps a selective mammalian retinotectal synapse instructed by Nephronectin. Nature Neuroscience, 25(5), pp659-674.
35434813	not listed	Kowal, T.J., et al. 2022. Distribution of prototypical primary cilia markers in subtypes of retinal ganglion cells. Journal of Comparative Neurology.
35429992	1:250	Marola, O.J., et al. 2022. Vascular derived endothelin receptor A controls endothelin-induced retinal ganglion cell death. Cell death discovery, 8(1), pp.1-10.
35414015	not listed	Guo, L.Y., et al. 2022. Multiplexed genome regulation in vivo with hyper-efficient Cas12a (pp. 1-11). Nature Publishing Group.
35401124	1:250	Abed, S., et al. 2022. Adult Expression of Tbr2 Is Required for the Maintenance but Not Survival of Intrinsically Photosensitive Retinal Ganglion Cells. Frontiers in Cellular Neuroscience, 16, p. 826590.
35135877	0.25ug/ml	Alarcon-Martinez, L., et al. 2022. Pericyte dysfunction and loss of interpericyte tunneling nanotubes promote neurovascular deficits in glaucoma. PNAS:USA, 119(7).
35040242	1:400	Zhang, N., et al. 2022. Using Methanol to Preserve Retinas for Immunostaining. Clinical and Experimental Ophthalmology.
34822767	1:500	Grimes, W.N., et al. 2021. Dendro-somatic synaptic inputs to ganglion cells contradict receptive field and connectivity conventions in the mammalian retina. Current Biology, 32(2), pp. 315-328.
34801667	1:1000	Di Pierdomenico, J., et al. 2021. Age and intraocular pressure in murine experimental glaucoma. Progress in retinal and eye research, p.101021.
34616039	1:500	Guttenplan, K.A., et al. 2021. Neurotoxic reactive astrocytes induce cell death via saturated lipids. Nature, 599(7883), pp.102-107.
34530842	1:500	Lazzara, F., et al. 2021. 1α, 25-dihydroxyvitamin D3 protects retinal ganglion cells in glaucomatous mice. Journal of neuroinflammation, 18(1), pp.1-19.
34187514	0.25ug/ml	Belforte, N., et al. 2021. AMPK hyperactivation promotes dendrite retraction, synaptic loss, and neuronal dysfunction in glaucoma. Molecular neurodegeneration, 16(1), pp.1-19.
33784498	1:1000	Johnson, K.P., et al. 2021. Cell-type-specific binocular vision guides predation in mice. Neuron.
33789891	1:500	Khatib, T.Z., et al. 2021. Receptor-ligand supplementation via a self-cleaving 2A peptide–based gene therapy promotes CNS axonal transport with functional recovery. Science Advances, 7(14), p.eabd2590.
33657370	1:500	Lindborg, J.A., et al. 2021. Optic nerve regeneration screen identifies multiple genes restricting adult neural repair. Cell Reports, 34(9), p.108777.
33434618	1:500	Shabanzadeh, A.P., et al. 2021. Cholesterol synthesis inhibition promotes axonal regeneration in the injured central nervous system. Neurobiology of Disease, p.105259.
33397711	1:1000	Pottackal, J., et al. 2021. Photoreceptive Ganglion Cells Drive Circuits for Local Inhibition in the Mouse Retina. Journal of Neuroscience, Jan 4, JN-RM-0674-20.
33293617	1:500	Groleau, M., et al. 2020. Mesoscopic cortical network reorganization during recovery of optic nerve injury in GCaMP6s mice. Scientific Reports, 10(1), pp.1-13.
33207201	1:500	Kerstein, P.C., et al. 2020. Gbx2 identifies two amacrine cell subtypes with distinct molecular, morphological, and physiological properties. Cell Reports, 33(7), p.108382.
33101779	1:200	Yu, H., et al. 2020. Mitochondrial transfer of the mutant human ND6T14484C gene causes visual loss and optic neuropathy. Translational Vision Science & Technology, 9(11), pp.1-1.
33045220	1:1000	Yabana, T., et al. 2020. GCaMP3 expressing cells in the ganglion cell layer of Thy1-GCaMP3 transgenic mice before and after optic nerve injury. Experimental Eye Research, p.108297.
33039455	1:300	Tao, X., et al. 2020. Single transient intraocular pressure elevations cause prolonged retinal ganglion cell dysfunction and retinal capillary abnormalities in mice. Experimental Eye Research, p.108296.
32910942	1:500	Flood, M.D., et al. 2020. Early diabetes impairs ON sustained ganglion cell light responses and adaptation without cell death or dopamine insensitivity. Experimental Eye Research, 200, p.108223.
32801314	1:500	de Araújo, D.S.M., et al. 2020. TRPA1 mediates damage of the retina induced by ischemia and reperfusion in mice. Cell Death & Disease, 11(8), pp.1-14.
32798466	1:500	Romano, G.L., et al. 2020. P2X7 receptor antagonism preserves retinal ganglion cells in glaucomatous mice. Biochemical Pharmacology, 180, p.114199.
32605122	1:500	Chou, T.H., et al. 2020. Nicotinamide-Rich Diet in DBA/2J Mice Preserves Retinal Ganglion Cell Metabolic Function as Assessed by PERG Adaptation to Flicker. Nutrients, 12(7), p.1910.
32494665	not listed	Prosseda, P.P., et al. 2020. Optogenetic stimulation of phosphoinositides reveals a critical role of primary cilia in eye pressure regulation. Science Advances, 6(18), p.eaay8699.
32457074	1:1000	Yan, W., et al. 2020. Mouse Retinal Cell Atlas: Molecular Identification of Over Sixty Amacrine Cell Types. Journal of Neuroscience, JN-RM-0471-20.
32029703	1:200	Mdzomba, J.B., et al. 2020. Nogo-A-targeting antibody promotes visual recovery and inhibits neuroinflammation after retinal injury. Cell Death and Disease, 11(2), pp.1-16.
31784286	not listed	Tran, N.M., et al. 2019. Single-Cell Profiles of Retinal Ganglion Cells Differing in Resilience to Injury Reveal Neuroprotective Genes. Neuron, 104(6), pp.1039-1055.
31775817	1:1000	Welsbie, D.S., et al. 2019. Targeted disruption of dual leucine zipper kinase and leucine zipper kinase promotes neuronal survival in a model of diffuse traumatic brain injury. Molecular Neurodegeneration, 14(1), p.44.
31722722	1:1000	Alrashdi, B., et al. 2019. Nav1. 6 promotes inflammation and neuronal degeneration in a mouse model of multiple sclerosis. Journal of Neuroinflammation, 16(1), pp.1-13.
31609468	not listed	Jiang, D., et al. 2019. Spatiotemporal gene expression patterns reveal molecular relatedness between retinal laminae. Journal of Comparative Neurology, 528(5), pp. 729-755.
30640971	1:1000	Blandford, S.N., et al. 2019. Retinal Characterization of the Thy1-GCaMP3 Transgenic Mouse Line After Optic Nerve Transection. Investigative Ophthalmology and Visual Science, 60(1), pp.183-191.
27391320	1:500	Ghinia, M.G., et al. 2019. Brn3a and Brn3b knockout mice display unvaried retinal fine structure despite major morphological and numerical alterations of ganglion cells. Journal of Comparative Neurology, 527(1), pp. 187-211.
30487225	1:300	Milosavljevic, N., et al. 2018. Photoreceptive retinal ganglion cells control the information rate of the optic nerve. Proceedings of the National Academy of Sciences, 115(50), pp.E11817-E11826.
30312782	1:2000	Yee, C.W., et al. 2018. Atypical expression and activation of GluN2A and GluN2B-containing NMDA receptors at ganglion cells during retinal degeneration. Neuroscience, 393, pp.61-72.
30197236	1:1000	Duan, X., et al. 2018. Cadherin Combinations Recruit Dendrites of Distinct Retinal Neurons to a Shared Interneuronal Scaffold. Neuron, 99(6), pp.1145-1154.
30076594	1:500	Lilley, B.N., et al. 2018. Genetic access to neurons in the accessory optic system reveals a role for Sema6A in midbrain circuitry mediating motion perception. Journal of Comparative Neurology, 527(1), pp.282-296.
30018341	not listed	Rheaume, B.A., et al. 2018. Single cell transcriptome profiling of retinal ganglion cells identifies cellular subtypes. Nature communications, 9(1), p.2759.
29937281	1:200	Drinnenberg, A., et al. 2018. How Diverse Retinal Functions Arise from Feedback at the First Visual Synapse. Neuron, 99(1), pp. 117-134.
29931057	1:1000	Agostinone, J., et al. 2018. Insulin signalling promotes dendrite and synapse regeneration and restores circuit function after axonal injury. Brain, 141(7), pp. 1963-1980.
29576390	1:50	Sarin, S., et al. 2018. Role for Wnt signaling in retinal neuropil development: analysis via RNA-seq and in vivo somatic CRISPR mutagenesis. Neuron, 98(1), pp.109-126.
29632360	1:500	Liu, J., et al. 2018. Tbr1 instructs laminar patterning of retinal ganglion cell dendrites. Nature Neuroscience, 21(5), pp. 659-670.
29439167	1:800	Ing-Esteves, S., et al. 2018. Combinatorial Effects of Alpha-and Gamma-Protocadherins on Neuronal Survival and Dendritic Self-Avoidance. Journal of Neuroscience, 38(11), pp.2713-2729.
28760865	1:500	Clements, R., et al. 2017. Dystroglycan maintains inner limiting membrane integrity to coordinate retinal development. Journal of Neuroscience, 37(35), pp. 8559-8574.
29241535	1:2000	Seabrook, T.A., et al. 2017. Strict Independence of Parallel and Poly-synaptic Axon-Target Matching during Visual Reflex Circuit Assembly. Cell Reports, 21(11), pp.3049-3064.
29106385	not listed	Livne-Bar, I., et al. 2017. Astrocyte-derived lipoxins A 4 and B 4 promote neuroprotection from acute and chronic injury. The Journal of Clinical Investigation, 127(12), pp.4403-4414.
28866734	1:4000	Ruzafa, N., et al. 2017. The Retina of Osteopontin deficient Mice in Aging. Molecular Neurobiology, 55(1), pp.213-221.
28781169	1:5000	Peng, Y.R., et al. 2017. Satb1 Regulates Contactin 5 to Pattern Dendrites of a Mammalian Retinal Ganglion Cell. Neuron, 95(4), pp. 869-883.
28607486	not listed	Sabbah, S., et al. 2017. A retinal code for motion along the gravitational and body axes. Nature, 546(7659), pp.492-497.
28472856	1:1000	Pérez de Sevilla Müller, L., et al. 2017. Multiple cell types form the VIP amacrine cell population. Journal of Comparative Neurology, 527(1), pp.133-158.
27699209	not listed	Ueki, Y., et al. 2016. Loss of Ikbkap causes slow, progressive retinal degeneration in a mouse model of Familial Dysautonomia. Eneuro, 3(5), p. ENEURO-0143.
27001178	not listed	Boggio, E. M., et al. 2016. Visual impairment in FOXG1-mutated individuals and mice. Neuroscience, 324,pp. 496-508.
25631988	1:20,000	Pérez de Sevilla Müller, L., et al. 2015. Expression and cellular localization of the voltage-gated calcium channel α2δ3 in the rodent retina. Journal of Comparative Neurology, 523(10), 1443-1460.

Immunohistochemistry: Primate
PMID	Dilution	Publication
34622958	1:500	Nasir‐Ahmad, S., et al. 2021. Satb1 expression in retinal ganglion cells of marmosets, macaques, and humans. Journal of Comparative Neurology, 530(6), pp 923-940.
34547460	1:500	McGregor, J.E., et al. 2021. Optogenetic therapy restores retinal activity in primate for at least a year following photoreceptor ablation. Molecular Therapy.
33527361	1:500	Nasir-Ahmad, S., et al. 2021. Identification of retinal ganglion cell types expressing the transcription factor Satb2 in three primate species. Journal of Comparative Neurology.
30377226	1:1000	Dhande, O.S., et al. 2019. Molecular Fingerprinting of On–Off Direction-Selective Retinal Ganglion Cells Across Species and Relevance to Primate Visual Circuits. Journal of Neuroscience, 39(1), pp. 78-95.
27568514	1:500	Long, Y., et al. 2016. Wide-field diffuse amacrine cells in the monkey retina contain immunoreactive Cocaine-and Amphetamine-Regulated Transcript (CART). Peptides, 84, pp. 22-35.

Immunohistochemistry: Rabbit
PMID	Dilution	Publication
30377226	1:1000	Dhande, O.S., et al. 2019. Molecular Fingerprinting of On–Off Direction-Selective Retinal Ganglion Cells Across Species and Relevance to Primate Visual Circuits. Journal of Neuroscience, 39(1), pp. 78-95.

Immunohistochemistry: Rat
PMID	Dilution	Publication
34819548	1:1000	Mathew, D.J., et al. 2021. An inducible rodent glaucoma model that exhibits gradual sustained increase in intraocular pressure with distinct inner retina and optic nerve inflammation. Scientific reports, 11(1), pp.1-16.
33789891	1:500	Khatib, T.Z., et al. 2021. Receptor-ligand supplementation via a self-cleaving 2A peptide–based gene therapy promotes CNS axonal transport with functional recovery. Science Advances, 7(14), p.eabd2590.
31369591	1:500	Choi, B.K., et al. 2019. Stabilization of primary cilia reduces abortive cell cycle re-entry to protect injured adult CNS neurons from apoptosis. PloS One, 14(8), p.e0220056.
31337818	1:1000	Kreymerman, A., et al. 2019. MTP18 is a Novel Regulator of Mitochondrial Fission in CNS Neuron Development, Axonal Growth, and Injury Responses. Scientific Reports, 9(1), p.10669.
27375437	1:500	Esquiva, G., et al. 2016. Non-image forming light detection by melanopsin, rhodopsin, and long-middlewave (L/W) cone opsin in the subterranean blind mole rat, Spalax ehrenbergi: immunohistochemical characterization, distribution, and connectivity. Frontiers in Neuroanatomy, 10, p.61.

Immunohistochemistry: Tree Shrew
PMID	Dilution	Publication
29238991	1:500	Johnson, E.N., et al. 2017. Distribution and diversity of intrinsically photosensitive retinal ganglion cells in tree shrew. Journal of Comparative Neurology, 527(1), pp.328-344.

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Anti-RBPMS Antibody

Our Anti-RBPMS primary antibody from PhosphoSolutions is guinea pig polyclonal. It detects guinea pig, human, mouse, non-human primate, rabbit, and rat RBPMS and is antigen affinity purified. It is great for use in WB, IHC, ICC.