Anti-Synaptotagmin-1 Antibody

Name: Anti-Synaptotagmin-1 Antibody
Brand: Aves Labs
SKU: STG
Price: 455.0 USD
Availability: InStock

4 Citations

Four different antipeptide antibodies were generated in chickens against sequences shared between the mouse (NP_033332) and human (NP_005630) gene products. Antibodies were affinity-purified and mixed in equal concentrations. These antibodies have been validated with human, mouse and rat tissues.

Immunohistochemical staining of Synaptotagmin-1 (green, 1:1000 dilution) and Vimentin (red, Rockland, 1:500 dilution) in a cryostat section through the basal plate of an e16 mouse brain.

Immunohistochemical staining of Synaptotagmin (green, 1:1000 dilution) and NFH (red, Rockland, 1:500 dilution) and Vimentin (blue) in a cryostat section through the Cochlear nerve and Organ of Corti in an adult mouse.

Immunohistochemical staining of Synaptotagmin (green, 1:1000 dilution) and NFH (red, Rockland, 1:500 dilution) in a cryostat section through the Cochlear nerve in an adult mouse.

Western blot of neonatal mouse brain homogenate. 25 ug of total protein added to each lane. Note the single band at the correct molecular weight. Hoda Ilias, Aves Labs.

Immunohistochemical staining of Synaptotagmin-1 (green, 1:1000 dilution) and Vimentin (red, Rockland, 1:500 dilution) in a cryostat section through the basal plate of an e16 mouse brain.

Species Reactivity

Human, Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Chicken

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from Aves Labs Datasheet

SKU: STG

Volume: 1000 µL

Variant

Price:

$455

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Product Details

Target

Synaptotagmin-1

Target Description

Human Synaptotagmin-1 is a 47,442 dalton protein (422 amino acids) associated with pre-synaptic terminals of the PNS and CNS. Synaptotagmin-1 binds calcium and initiates the fusion of vesicles with the pre-synaptic membrane. This protein also is believed to be the target of proteolytic activity associated with Botulinum neurotoxin A.

Format

Affinity-Purified IgY

Concentration

400 µg/mL

Clonality

Polyclonal

Isotype

IgY

Applications

ICC, IHC, WB

Host Species

Chicken

Gene Name

SYT1 SVP65 SYT

Molecular Weight

48 kDa

Antigen

Synthetic peptides

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2313562

Storage

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.

Physical State

Liquid

Production Notes

Chickens were immunized with four synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. These synthetic peptides corresponded to different regions of the Synaptotagmin-1 gene product, but are shared between the human (NP_005630, NCBI) and mouse (NP_033332, NCBI) sequences. After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentra-tions of the eluates adjusted to 400 µg/mL. Finally, equal volumes of each of the three affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.

Buffer

Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS," pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.

Dilution Ranges

WB: 1:1000-1:2000
IHC: 1:1000-1:2000
ICC: 1:1000-1:2000

Quality Control

Each of the three antibodies were analyzed by immunohistochemistry (at a dilution of 1:2000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

12 months from receipt of product

Shipping

Ice packs

Synonyms

Synaptotagmin-1 (Synaptotagmin I) (SytI) (p65)

UniProt Details

UniProt (Immunogen Species): P21579

Product Specific References for Applications and Species

Immunohistochemistry: Mouse
Western Blot: Bovine | Rat

Immunohistochemistry: Mouse
PMID	Dilution	Publication
29536590	1:1,000	Harley, R.J., et al. 2018. Neuronal Cell Adhesion Molecule (NrCAM) is Expressed by Sensory Cells in the Cochlea and is Necessary for Proper Cochlear Innervation and Sensory Domain Patterning During Development. Developmental Dynamics, 934-950.
29059194	1:1,000	Salehi, P., et al. 2017. Role of Neuropilin-1/Semaphorin-3A signaling in the functional and morphological integrity of the cochlea. Plos Genetics, e1007048.

Western Blot: Bovine
PMID	Dilution	Publication
30383978	1:3000	Weston, M.R., et al. 2018. Characterizing chloride-dependent acidification in brain Clathrin-coated vesicles. Biochemistry and Cell Biology, 315-324.

Western Blot: Rat
PMID	Dilution	Publication
21916746	not listed	Sriram, K., et al. 2011. Neurotoxicity following acute inhalation exposure to the oil dispersant COREXIT EC9500A. Journal of Toxicology and Environmental Health. Part A, 1405-1418.

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Anti-Synaptotagmin-1 Antibody

Four different antipeptide antibodies were generated in chickens against sequences shared between the mouse (NP_033332) and human (NP_005630) gene products. Antibodies were affinity-purified and mixed in equal concentrations. These antibodies have been validated with human, mouse and rat tissues.

SKU: STG

Product Details

Product Specific References for Applications and Species

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