Immunofluorescence of human iPSC-derived dopaminergic neurons labeled with anti-tyrosine hydroxylase (cat # 2025-THRAB, green, 1:500). The blue stain is DAPI. Before labeling, cells were fixed with 4% PFA and permeabilized with 0.1 to 0.3 % Triton X. This image is kindly provided by Aurelie de Rus Jacquet, Howard Hughes Medical Institute, Janelia Research Campus.
Immunostaining of E17 rat midbrain mixed neuronal cultures showing TH positive neurons (cat # 2025-THRAB, green, 1:500) and MAP2 (cat #1100-MAP2, red, 1:2000). Before labeling, cells were fixed with 4% PFA in PBS and permeabilized for 1 hour 0.3 % Triton X. Image courtesy of Aurélie de Rus Jacquet, laboratory of Dr. Jean-Christophe Rochet, Purdue University.
Tyrosine hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines dopamine and norepinephrine. TH antibodies can therefore be used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson’s disease and drug abuse (Kish et al., 2001; Zhu et al., 2000; Zhu et al., 1999). TH antibodies can also be used to explore basic mechanisms of dopamine and norepinephrine signaling (Witkovsky et al., 2000; Salvatore et al., 2001; Dunkley et al., 2004).
Volume: 100 µL
Concentration: Lot Specific
Form: Antigen Affinity Purified from Pooled Serum
Host Species: Rabbit
Applications: WB, IHC
Immunogen: SDS-denatured, native rat tyrosine hydroxylase purified from pheochromocytoma
Gene ID: TH
Physical State: Liquid
Buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
Validation and Application Notes
Molecular Weight: 60
Aves Labs products are intended for use as research laboratory reagents. They are not intended for use as diagnostic or therapeutic reagents in humans.