Name: Anti-Tyrosine Hydroxylase Antibody
Brand: PhosphoSolutions
SKU: 2027-THSHP
Price: 415.0 USD
Availability: InStock

Our Anti-Tyrosine Hydroxylase sheep polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects all mammalian Tyrosine Hydroxylase and is antigen affinity purified from pooled serum. It is great for use in WB, IHC, ICC.

Product Type

Primary Antibody

Species Reactivity

Human, Mouse, Rabbit, Rat

Expected Reactivity

Chicken

Applications

ICC, IHC, WB

Host Species

Sheep

Format

Antigen Affinity Purified from Pooled Serum

Gene Name

TH

Molecular Weight

60 kDa

Go to Full Product Details

Western blot of 10ug of rat striatal lysate showing specific immunolabeling of the ~60 kDa tyrosine hydroxylase protein.

Immunostaining of rabbit retina showing specific labeling of tyrosine hydroxylase in green.

Volume: 100 µL

Variant

Price:

$415

Quantity:

Bulk Order Anti-Tyrosine Hydroxylase Antibody

Product Details

Target

Tyrosine Hydroxylase

Target Description

Tyrosine hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines dopamine and norepinephrine. TH antibodies can therefore be used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson’s disease and drug abuse (Kish et al., 2001; Zhu et al., 2000; Zhu et al., 1999). TH antibodies can also be used to explore basic mechanisms of dopamine and norepinephrine signaling (Witkovsky et al., 2000; Salvatore et al., 2001).

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

ICC, IHC, WB

Host Species

Sheep

Gene Name

TH

Molecular Weight

60 kDa

Antigen

SDS-denatured, native rat tyrosine hydroxylase purified from pheochromocytoma.

Species Reactivity

Human, Mouse, Rabbit, Rat

Expected Reactivity

Chicken

Antibody Registry ID

AB_2492277

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled sheep serum by affinity purification using a column to which the fusion protein immunogen was coupled.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000
WB Brain: 1:1000
IHC: 1:1000
ICC: 1:1000

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~60 kDa tyrosine hydroxylase protein.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

Dystonia 14 antibody, DYT14 antibody, DYT5b antibody, EC 1.14.16.2 antibody, OTTHUMP00000011225 antibody, OTTHUMP00000011226 antibody, ple antibody, Protein Pale antibody, TH antibody, The antibody, TY3H_HUMAN antibody, TYH antibody, Tyrosine 3 hydroxylase antibody, Tyrosine 3 monooxygenase antibody, Tyrosine 3-hydroxylase antibody, Tyrosine 3-monooxygenase antibody, Tyrosine hydroxylase antibody

UniProt Details

UniProt (Human): P07101
UniProt (Immunogen Species): P04177

NCBI Gene ID

25085

Product Specific References for Applications and Species

Immunohcytochemistry: Mouse
Immunohistochemistry: Mouse
Western Blotting: Human

Immunocytochemistry: Mouse
PMID	Dilution	Publication
34725102	1:200	Sekar, A., et al. 2021. RapID Cell Counter: Semi-automated and mid-throughput estimation of cell density within diverse cortical layers. Eneuro, 8(6).

Immunohistochemistry: Mouse
PMID	Dilution	Publication
34151467	1:1000	Cardenas, A., et al. 2021. The role of medial prefrontal cortex projections to locus ceruleus in mediating the sex differences in behavior in mice with inflammatory pain. The FASEB Journal, 35(7), p.e21747.
29584618	not listed	Ryu, J.C., et al. 2018. Role of proNGF/p75 signaling in bladder dysfunction after spinal cord injury. The Journal of Clinical Investigation, May 1;128(5):1772-1786.

Western Blotting: Human
PMID	Dilution	Publication
12065667	not listed	Waymire, J.C. and Haycock, J.W. 2002. . Lack of regulation of aromatic l‐amino acid decarboxylase in intact bovine chromaffin cells. Journal of Neurochemistry, 81(3), pp.589-593.

Product Specific Protocols

Western Blot Protocol: Download

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Anti-Tyrosine Hydroxylase Antibody

Our Anti-Tyrosine Hydroxylase sheep polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects all mammalian Tyrosine Hydroxylase and is antigen affinity purified from pooled serum. It is great for use in WB, IHC, ICC.