Name: Anti-Tyrosine Hydroxylase (Ser40) Antibody
Brand: PhosphoSolutions
SKU: p1580-40
Price: 415.0 USD
Availability: InStock

Our Anti-Tyrosine Hydroxylase (Ser40) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects many mammalian, and many non-mammalian Tyrosine Hydroxylase (Ser40) and is antigen affinity purified from pooled serum. It is great for use in WB, IHC, ICC.

Product Type

Primary Antibody

Applications

ICC, IHC, WB

Host Species

Rabbit

Format

Antigen Affinity Purified from Pooled Serum

Gene Name

TH

Molecular Weight

60 kDa

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Immunostaining of light-stimulated rabbit retina showing labeling of TH when phosphorylated at Ser40.

Western blot of recombinant phospho-TH and non-phospho-TH showing selective immunolabeling by the phosphospecific antibody of the ~60 kDa TH phosphorylated at Ser40. The pan-specific antibody (anti-pan-TH) recognized both the phospho- and non-phospho-TH; while most importantly, the phospho-specific antibody (anti-Ser40 TH) recognized only phospho-TH.

Volume: 100 µL

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Price:

$415

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Product Details

Target

Tyrosine Hydroxylase (Ser40)

Target Description

Tyrosine hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines Dopamine and Norepinephrine. TH antibodies can therefore be used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson’s disease and drug abuse (Kish et al., 2001; Zhu et al., 2000; Zhu et al., 1999). TH antibodies can also be used to explore basic mechanisms of dopamine and norepinephrine signaling (Witkovsky et al., 2000; Salvatore et al., 2001; Dunkley et al., 2004). The activity of TH is also regulated by phosphorylation (Haycock et al., 1982; Haycock et al., 1992; Jedynak et al., 2002). Phospho-specific antibodies for the phosphorylation sites on TH can be used to great effect in studying this regulation and in identifying the cells in which TH phosphorylation occurs.

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

ICC, IHC, WB

Host Species

Rabbit

Gene Name

TH

Molecular Weight

60 kDa

Antigen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser40 of rat tyrosine hydroxylase, conjugated to keyhole limpet hemocyanin (KLH).

Antibody Registry ID

AB_2492279

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000
WB Brain: 1:1000
IHC: 1:1000
ICC: 1:50-1:1000

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~60 kDa tyrosine hydroxylase protein phosphorylated at Ser40. Some higher molecular weight bands may be detected by the antibody depending upon the brain region being studied, protein loads and the detection methods used. The antibody has three orders of magnitude selectivity over non-phospho TH.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Ser40

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

Dystonia 14 antibody, DYT14 antibody, DYT5b antibody, EC 1.14.16.2 antibody, OTTHUMP00000011225 antibody, OTTHUMP00000011226 antibody, ple antibody, Protein Pale antibody, TH antibody, The antibody, TY3H_HUMAN antibody, TYH antibody, Tyrosine 3 hydroxylase antibody, Tyrosine 3 monooxygenase antibody, Tyrosine 3-hydroxylase antibody, Tyrosine 3-monooxygenase antibody, Tyrosine hydroxylase antibody

UniProt Details

UniProt (Immunogen Species): P04177

NCBI Gene ID

25085

Product Specific References for Applications and Species

Immunocytochemistry: Human | Rat
Western Blot: Human | Mouse | Rat

Immunocytochemistry: Human
PMID	Dilution	Publication
28637871	1:50	Jorge-Finnigan, A., et al. 2017. Phosphorylation at serine 31 targets tyrosine hydroxylase to vesicles for transport along microtubules. Journal of Biological Chemistry, 292(34), pp.14092-14107.

Immunocytochemistry: Rat
PMID	Dilution	Publication
32778969	1:3000	Nakashima, A., et al. 2020. NT5DC2 affects the phosphorylation of tyrosine hydroxylase regulating its catalytic activity. Journal of Neural Transmission, pp.1-10.
28637871	1:50	Jorge-Finnigan, A., et al. 2017. Phosphorylation at serine 31 targets tyrosine hydroxylase to vesicles for transport along microtubules. Journal of Biological Chemistry, 292(34), pp.14092-14107.
10936746	1:300	Witkovsky, P, et al. 2000. Influence of light and neural circuitry on tyrosine hydroxylase phosphorylation in the rat retina. Journal of chemical neuroanatomy, 19(2), pp.105-116.

Western Blot: Human
PMID	Dilution	Publication
28637871	1:1000	Jorge-Finnigan, A., et al. 2017. Phosphorylation at serine 31 targets tyrosine hydroxylase to vesicles for transport along microtubules. Journal of Biological Chemistry, 292(34), pp.14092-14107.
11677263	not listed	Salvatore, M.F, et al. 2001. Depolarization‐stimulated catecholamine biosynthesis: involvement of protein kinases and tyrosine hydroxylase phosphorylation sites in situ. Journal of neurochemistry, 79(2), pp.349-360.

Western Blot: Mouse
PMID	Dilution	Publication
30803445	1:1000	Areal, L.B., et al. 2019. Neuronal scaffolding protein spinophilin is integral for cocaine-induced behavioral sensitization and ERK1/2 activation. Molecular brain, 12(1), p.15.
29782882	1:1000	Salvatore, M.F., et al. 2018. Prolonged increase in ser31 tyrosine hydroxylase phosphorylation in substantia nigra following cessation of chronic methamphetamine. Neurotoxicology, 67, pp.121-128.
29299922	not listed	Bezem, M.T., et al. 2018. Stabilization of human tyrosine hydroxylase in maltodextrin nanoparticles for delivery to neuronal cells and tissue. Bioconjugate Chemistry Feb 21;29(2):493-502.
27124386	not listed	Salvatore, M.F., et al. 2016. Regulation of tyrosine hydroxylase expression and phosphorylation in dopamine transporter-deficient mice. ACS chemical neuroscience, 7(7), pp.941-951.
19017804	not listed	Meyer, D.A., et al. 2008. Striatal dysregulation of Cdk5 alters locomotor responses to cocaine, motor learning, and dendritic morphology. PNAS, 105: 18561 - 18566.

Western Blot: Rat
PMID	Dilution	Publication
32778969	not listed	Nakashima, A., et al. 2020. NT5DC2 affects the phosphorylation of tyrosine hydroxylase regulating its catalytic activity. Journal of Neural Transmission, pp.1-10.
28637871	1:1000	Jorge-Finnigan, A., et al. 2017. Phosphorylation at serine 31 targets tyrosine hydroxylase to vesicles for transport along microtubules. Journal of Biological Chemistry, 292(34), pp.14092-14107.
28637176	1:1000	Salvatore, M.F., et al. 2017. Dissociation of striatal dopamine and tyrosine hydroxylase expression from aging-related motor decline: evidence from calorie restriction intervention. Journals of Gerontology Series A: Biomedical Sciences and Medical Sciences, Dec 12;73(1):11-20.
26777664	1:1000	Baroso-Chinea, P., et al. 2016. Long-term controlled GDNF over-expression reduces dopamine transporter activity without affecting tyrosine hydroxylase expression in the mesostriatal system rat. Neurobiology of Disease, 88:44-54.
24743698	not listed	Izumi, Y, et al. 2014. Endogenous dopamine is involved in the herbicide paraquat-induced dopaminergic cell death. Toxicological Sciences, 139(2), pp.466-478.
24410633	1:1000	Salvatore, M.F., et al. 2014. ser31 tyrosine hydroxylase phosphorylation parallels differences in dopamine recovery in nigrostriatal pathway following 6‐OHDA lesion. Journal of neurochemistry, 129(3), 548-558.
22723669	1:100,000	McCutcheon, J.E., et al. 2012. Dopamine neurons in the ventral tegmental area fire faster in adolescent rats than in adults. J Neurophysiology 108(6): 1620-1630.
22458761	1:2000	Calipari, E.S., et al. 2014. Methylphenidate and cocaine self‐administration produce distinct dopamine terminal alterations. Addiction biology, 19(2), pp.145-155.
12124439	1:1500	Jedynak, J.P., et al. 2002. Acute administration of cocaine regulates the phosphorylation of serine‐19,‐31 and‐40 in tyrosine hydroxylase. Journal of neurochemistry, 82(2), pp.382-388.
11677263	not listed	Salvatore, M.F, et al. 2001. Depolarization‐stimulated catecholamine biosynthesis: involvement of protein kinases and tyrosine hydroxylase phosphorylation sites in situ. Journal of neurochemistry, 79(2), pp.349-360.

Product Specific Protocols

Western Blot Protocol: Download

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Anti-Tyrosine Hydroxylase (Ser40) Antibody

Our Anti-Tyrosine Hydroxylase (Ser40) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects many mammalian, and many non-mammalian Tyrosine Hydroxylase (Ser40) and is antigen affinity purified from pooled serum. It is great for use in WB, IHC, ICC.