Our Anti-V5 chicken polyclonal primary antibody from Aves Labs is produced in-house. It detects V5, and is affinity purified. It is great for use in ICC, WB.
Immunofluoresence of COS7 cells expressing a V5-tagged expression plasmid and then stained with chicken anti-V5 antibodies (green) and the leading mouse anti-V5 antibody (red). Blue is DAPI nuclear stain showing nuclei of both transfected and untransfected cells. Staining shows 100% correspondence between the two antibodies for recognition of transfected cells.
The V5 epitope tag is derived from a protein epitope found in the P and V proteins of paramyxovirus from the simian virus 5 family. The V5 tag is generally added as an epitope tag to proteins using all 14 amino acids (GKPIPNPLLGLDST), but it may also be used with a shorter 9-amino acid (IPNPLLGLD) sequence. Proteins tagged with the V5 epitope tag are detected with high sensitivity and specificity using our chicken anti-V5 antibody using various applications including western blot, immunocytochemistry, and immunohistochemistry.
ICC, IHC, WB
Synthetic peptide encoding 14 amino acid V5 epitope fused to KLH carrier protein
Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.
Eggs from hens hyperimmunized with target were used to prepare an IgY fraction which was then subjected to antigen-specific affinity purification
Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS," pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.
Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used in Western blot on a V5 overexpression cell lysate.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.