BlokHen® Blocking Reagent

Our proprietary blocking reagent, BlokHen® is a fish serum-based immunoreagent designed for use with chicken antibodies. It already contains concentrated buffer and non-ionic detergent (tween-20), allowing simple dilution with water. BlokHen® can be used in Western Blots, Immunohistochemistry or Immunocytochemistry, Immunoprecipitation, or ELISA applications.

Blocking Reagent

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BlokHen® Blocking Reagent
SKU: BH-1001

Volume: 100 mL
Sale price$225

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Product Specific References for Applications and Species

ELISA: Chicken
PMID Dilution Publication
251319911.0%Anji, A., et al. 2015. Expression of α‐subunit of α‐glucosidase II in adult mouse brain regions and selected organs. Journal of Neuroscience Research, 82-93.
Immunocytochemistry: Canine
PMID Dilution Publication
2127720710%Berg, Z., et al. 2011. Muscle satellite cells from GRMD dystrophic dogs are not phenotypically distinguishable from wild type satellite cells in ex vivo culture. Neuromuscular Disorders, 282-290.
Immunocytochemistry: Human
PMID Dilution Publication
344524091:2000Funk, C., et al. 2021. The HSV1 Tail-Anchored Membrane Protein pUL34 Contains a Basic Motif That Supports Active Transport to the Inner Nuclear Membrane Prior to Formation of the Nuclear Egress Complex. Viruses, 1544.
3030179910%Katsumura, K.R., et al. 2018. Human leukemia mutations corrupt but do not abrogate GATA-2 function. PNAS: USA, E10109-10118.
283704121:10Kamalakar, A., et al. 2017. PTHrP (12‐48) Modulates the Bone Marrow Microenvironment and Suppresses Human Osteoclast Differentiation and Lifespan. Journal of Bone and Mineral Research, 1421-1431.
2835137320%Guan, F.H., et al. 2017. The antiproliferative ELF2 isoform, ELF2B, induces apoptosis in vitro and perturbs early lymphocytic development in vivo. Journal of Hematology and Oncology, 75.
2831906220%Marshall, A.D., et al. 2017. CTCF genetic alterations in endometrial carcinoma are pro-tumorigenic. Oncogene, 4100-4110.
281422055%Bajpai, V.K., et al. 2017. Reprogramming postnatal human epidermal keratinocytes toward functional neural crest fates. Stem Cells , 1402-1415.
2010691710%Roller, R.J., et al. 2010. Analysis of a charge cluster mutation of herpes simplex virus type 1 UL34 and its extragenic suppressor suggests a novel interaction between pUL34 and pUL31 that …. Journal of Virology, 3921-3924.
19934382not listedYuan, L., et al. 2010. A stretch of 17 amino acids in the prosaposin C terminus is critical for its binding to sortilin and targeting to lysosomes. The Journal of Histochemistry and Cytochemistry, 287-300.
Immunocytochemistry: Mouse
PMID Dilution Publication
362142541:1000LaCombe, R., et al. 2022. EphA1 Receptor Tyrosine Kinase is Localized to the Nucleus in Rhabdomyosarcoma from Multiple Species. Biology Open, .
26305592not listedGarcia-Gonzalo, F.R., et al. 2015. Phosphoinositides regulate ciliary protein trafficking to modulate hedgehog signaling. Developmental Cell, 400-409.
1837142110%Brack, A.S., et al. 2008. A temporal switch from notch to Wnt signaling in muscle stem cells is necessary for normal adult myogenesis. Cell Stem Cell, 50-59.
1830830210%Capkovic, K.L., et al. 2008. Neural cell adhesion molecule (NCAM) marks adult myogenic cells committed to differentiation. Experimental Cell Research, 1553-1565.
1765238810%Leach, N., et al. 2007. Emerin is hyperphosphorylated and redistributed in herpes simplex virus type 1-infected cells in a manner dependent on both UL34 and US3. Journal of Virology, 10792-10803.
Immunocytochemistry: Rat
PMID Dilution Publication
18759491not listedMalarkey, E.B., et al. 2008. Water soluble single-walled carbon nanotubes inhibit stimulated endocytosis in neurons. Nano Letters, 3538-3542.
Immunohistochemistry: Canine
PMID Dilution Publication
3052065710%Landry, G.M., et al. 2019. Cloning, function, and localization of human, canine, and Drosophila ZIP10 (SLC39A10), a Zn2+ transporter. American Journal of Physiology and Renal Physiology, F263-F273.
Immunohistochemistry: Chicken
PMID Dilution Publication
1962798710%Beazley, K.E., et al. 2009. Developmental regulation of the nuclear ferritoid–ferritin complex of avian corneal epithelial cells: Roles of systemic factors and thyroxine. Experimental Eye Research, 854-862.
Immunohistochemistry: Drosophila
PMID Dilution Publication
3052065710%Landry, G.M., et al. 2019. Cloning, function, and localization of human, canine, and Drosophila ZIP10 (SLC39A10), a Zn2+ transporter. American Journal of Physiology and Renal Physiology, F263-F273.
2701762410%Sanghavi, P., et al. 2016. Multiple Roles for Egalitarian in Polarization of the Drosophila Egg Chamber. Genetics, 415-432.
Immunohistochemistry: Human
PMID Dilution Publication
3052065710%Landry, G.M., et al. 2019. Cloning, function, and localization of human, canine, and Drosophila ZIP10 (SLC39A10), a Zn2+ transporter. American Journal of Physiology and Renal Physiology, F263-F273.
2005433810%Liu, Y., et al. 2010. Regulation of the psoriatic chemokine CCL20 by E3 ligases Trim32 and Piasy in keratinocytes. Journal of Investigative Dermatology, 1384-1390.
198623395%Iragavarapu, S., et al. 2009. ETX1 is over-expressed in the glaucomatous trabecular meshwork. Molecular Vision, 2061-2067.
1842476820%Kowalczuk, S., et al. 2008. A protein complex in the brush‐border membrane explains a Hartnup disorder allele. FASEB, 2880-2887.
Immunohistochemistry: Mouse
PMID Dilution Publication
36932080not listedBerry, M.H., et al. 2023. A Melanopsin Ganglion Cell Subtype Forms a Dorsal Retinal Mosaic Projecting to the Supraoptic Nucleus. Nature Communications, 1492.
3693607410%Xing, Y.L., et al. 2023. High-Efficiency Pharmacogenetic Ablation of Oligodendrocyte Progenitor Cells in the Adult Mouse CNS. Cell Reports Methods, 100414.
367089497.5%Nayak, S., et al. 2023. Chromatin landscape governing murine epidermal differentiation. The Journal of Investigative Dermatology, .
362870381:500Zhang, W., et al. 2022. Bone Metastasis Initiation is Coupled with Bone Remodeling Through Osteogenic Differentiation of NG2+ Cells. Cancer Discovery, 474-495.
352760961%Gadomski, S., et al. 2022. A cholinergic neuroskeletal interface promotes bone formation during postnatal growth and exercise. Cell Stem Cell, 528-544.
351925461%Valet, C., et al. 2022. Sepsis promotes splenic production of a protective platelet pool with high CD40 ligand expression. The Journal of Clinical Investigation, e153920.
350870601%Fielding, C., et al. 2022. Cholinergic signals preserve haematopoietic stem cell quiescence during regenerative haematopoiesis. Nature Communication, 543.
33338666not listedKhalid, A.B., et al. 2021. GATA4 regulates mesenchymal stem cells via direct transcriptional regulation of the WNT signalosome. Bone, 115819.
3052065710%Landry, G.M., et al. 2019. Cloning, function, and localization of human, canine, and Drosophila ZIP10 (SLC39A10), a Zn2+ transporter. American Journal of Physiology and Renal Physiology, F263-F273.
2953659010%Harley, R.J., et al. 2018. Neuronal cell adhesion molecule (NrCAM) is expressed by sensory cells in the cochlea and is necessary for proper cochlear innervation and sensory domain …. Developmental Dynamics, 934-950.
2733939310%Hegde, S., et al. 2016. Phosphodiesterase 11A (PDE11A), enriched in ventral hippocampus neurons, is required for consolidation of social but not nonsocial memories in mice. Neuropsychopharmacology, 2920-2931.
26962307not listedWang, P.R., et al. 2016. Cellular origins of regenerating nodules and malignancy in the FAH model of liver injury after bone marrow cell transplantation. Stem Cells International, 5791317.
2630220610%Coate, T.M., et al. 2015. Neuropilin-2/Semaphorin-3F-mediated repulsion promotes inner hair cell innervation by spiral ganglion neurons. Elife, e07830.
2531970810%Xing, Y.L., et al. 2014. Adult neural precursor cells from the subventricular zone contribute significantly to oligodendrocyte regeneration and remyelination. Journal of Neuroscience, 14128-14146.
252677917.5%Dema, B., et al. 2014. Immunoglobulin E plays an immunoregulatory role in lupus. Journal of Experimental Medicine, 2159-2168.
2163657620%Bröer, A., et al. 2011. Impaired nutrient signaling and body weight control in a Na+ neutral amino acid cotransporter (Slc6a19)-deficient mouse. Journal of Biological Chemistry, 26638-26651.
201513651%White, R.E., et al. 2010. Regional heterogeneity in astrocyte responses following contusive spinal cord injury in mice. Journal of Comparative Neurology, 1370-1390.
19711380not listedYee, C.L., et al. 2009. Arcuate nucleus expression of NKX2. 1 and DLX and lineages expressing these transcription factors in neuropeptide Y+, proopiomelanocortin+, and tyrosine …. Journal of Comparative Neurology, 37-50.
196570211:10Liu, M.T., et al. 2009. 5-HT4 receptor-mediated neuroprotection and neurogenesis in the enteric nervous system of adult mice. Journal of Neuroscience, 9683-9699.
Immunohistochemistry: Rat
PMID Dilution Publication
29454014not listedGluck, M.J., et al. 2018. Detecting structural and inflammatory response after in vivo stretch injury in the rat median nerve via second harmonic generation. Journal of Neuroscience Methods, 68-80.
2303182510%Coutts, D.J., et al. 2013. Embryonic-derived olfactory ensheathing cells remyelinate focal areas of spinal cord demyelination more efficiently than neonatal or adult-derived cells. Cell Transplantation, 1249-1261.
2213817910%Dmitrieff, E.F., et al. 2012. Carotid body growth during chronic postnatal hyperoxia. Respiratory Physiology and Neurobiology, 193-203.
2109428210%Dmitrieff, E.F., et al. 2011. Chronic hyperoxia alters the expression of neurotrophic factors in the carotid body of neonatal rats. Respiratory Physiology and Neurobiology, 220-227.
Western Blot: Human
PMID Dilution Publication
283704121:10Kamalakar, A., et al. 2017. PTHrP (12‐48) Modulates the Bone Marrow Microenvironment and Suppresses Human Osteoclast Differentiation and Lifespan. Journal of Bone and Mineral Research, 1421-1431.
Western Blot: Mouse
PMID Dilution Publication
1747042610%Hwang, J., et al. 2007. Role of Scarf and its binding target proteins in epidermal calcium homeostasis. Journal of Biological Chemistry, 18645-18653.
1007597810%Schneider, T.J., et al. 1999. A novel gene coding for a Fas apoptosis inhibitory molecule (FAIM) isolated from inducibly Fas-resistant B lymphocytes. Journal of Experimental Medicine, 949-956.
Additional Publications: Unspecified
PMID Publication
28884467Sundberg, S.C., et al. 2018. Cre‐expressing neurons in visual cortex of Ntsr1‐Cre GN220 mice are corticothalamic and are depolarized by acetylcholine. Journal of Comparative Neurology, 120-132.
21071039Lee, J.H., et al. 2011. Schwann cell-like remyelination following transplantation of human umbilical cord blood (hUCB)-derived mesenchymal stem cells in dogs with acute spinal cord injury. Journal of the Neurological Sciences, 86-96.
17346701Loitto, V.M., et al. 2007. Filopodia are induced by aquaporin-9 expression. Experimental Cell Research, 1295-1306.
17295233Hudson, L.G., et al. 2007. Ultraviolet radiation stimulates expression of Snail family transcription factors in keratinocytes. Molecular Carcinogenesis, 257-268.
17200142Sigal, Y.J., et al. 2007. Cdc42 and ARP2/3-independent regulation of filopodia by an integral membrane lipid-phosphatase-related protein. Journal of Cell Science, 340-352.
16969078Hnasko, R., et al. 2006. PV-1 labels trans-cellular openings in mouse endothelial cells and is negatively regulated by VEGF. Cell Cycle, 2021-2028.
16513825Peluso, J.J., et al. 2006. Progesterone membrane receptor component 1 expression in the immature rat ovary and its role in mediating progesterone's antiapoptotic action. Endocrinology, 3133-3140.
16427676Bjerke, S.L., et al. 2006. Roles for herpes simplex virus type 1 UL34 and US3 proteins in disrupting the nuclear lamina during herpes simplex virus type 1 egress. Virology, 261-276.
16216560Held, P.K., et al. 2006. In vivo genetic selection of renal proximal tubules. Molecular Therapies, 49-58.
16154690Reiman, R., et al. 2005. Expression of C5a in the brain does not exacerbate experimental autoimmune encephalomyelitis. Neuroscience Newsletters, 134-138.
16153295Franklin, R.B., et al. 2005. hZIP1 zinc uptake transporter down regulation and zinc depletion in prostate cancer. Molecular Cancer, 32.
16037544Liu, M., et al. 2005. Expression and function of 5-HT4 receptors in the mouse enteric nervous system. American Journal of Physiology. Gastrointestinal and Liver Physiology, G1148-G1163.
15951442Poliakov, E., et al. 2005. Key role of conserved histidines in recombinant mouse β-carotene 15, 15′-monooxygenase-1 activity. Journal of Biological Chemistry, 29217-29223.
15914599Lu, X., et al. 2005. Identification of novel murine-and human-specific RPGRIP1 splice variants with distinct expression profiles and subcellular localization. Investigative Opthalmology and Visual Science, 1882-1890.
15862180Bhattacharya, S.K., et al. 2005. Cochlin deposits in the trabecular meshwork of the glaucomatous DBA/2J mouse. Experimental Eye Research, 741-744.
15814896Peluso, J.J., et al. 2005. Expression and function of PAIRBP1 within gonadotropin-primed immature rat ovaries: PAIRBP1 regulation of granulosa and luteal cell viability. Biology Reproduction, 261-270.
15640522Hnasko, R., et al. 2005. Developmental regulation of PV-1 in rat lung: association with the nuclear envelope and limited colocalization with Cav-1. American Journal of Physiology, L275-284.
15579465Bhattacharya, S.K., et al. 2005. Proteomics reveal Cochlin deposits associated with glaucomatous trabecular meshwork. Journal of Biological Chemistry, 6080-6084.
15501225Olguin, H.C., et al. 2004. Pax-7 up-regulation inhibits myogenesis and cell cycle progression in satellite cells: a potential mechanism for self-renewal. Developmental Biology, 375-388.
15371336Cornelison, D.D.W., et al. 2004. Essential and separable roles for Syndecan-3 and Syndecan-4 in skeletal muscle development and regeneration. Genes and Development, 2231-2236.
15265785Gwynn, B., et al. 2004. Reduced pigmentation (rp), a mouse model of Hermansky-Pudlak syndrome, encodes a novel component of the BLOC-1 complex. Blood, 3181-3189.
14988380Peluso, J.J., et al. 2004. Involvement of an unnamed protein, RDA288, in the mechanism through which progesterone mediates its antiapoptotic action in spontaneously immortalized …. Endocrinology, 3014-3022.
14724813Hase, K., et al. 2003. Expression of LL-37 by human gastric epithelial cells as a potential host defense mechanism against Helicobacter pylori. Gastroenterology, 1613-1625.
12958441Matson, C.T., et al. 2003. Immunoblot detection of brain vascular proteins. Methods in Molecular Medicine, 479-487.
12805460Bjerke, S.L., et al. 2003. Effects of Charged Cluster Mutations on the Function of Herpes Simplex Virus Type 1 UL34 Protein. Journal of Virology, 7601-7610.
11356697Jeffs, B., et al. 2001. Sertoli Cell-Specific Rescue of Fertility, But Not Testicular Pathology, in Dax1 (Ahch)-Deficient Male Mice. Endocrinology, 2481-2488.
10677043Li, H.S., et al. 1999. Activation of a TRPC3-dependent cation current through the neurotrophin BDNF. Neuron, 261-273.
10362594Raman, N., et al. 1999. Rho controls actin cytoskeletal assembly in renal epithelial cells during ATP depletion and recovery. American Journal of Physiology, C1312-C1324.

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